A5001349470- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Carbohydrate Chemistry and Synthesis
- Glycosylation and Glycoproteins Research
- Crystallography and molecular interactions
- Organic Chemistry Cycloaddition Reactions
- DNA and Nucleic Acid Chemistry
- Genetics and Neurodevelopmental Disorders
- Chemical Synthesis and Analysis
- Cell Adhesion Molecules Research
- Protein Kinase Regulation and GTPase Signaling
- Organophosphorus compounds synthesis
- Synthesis of Organic Compounds
- Metal complexes synthesis and properties
- Synthesis and Reactivity of Sulfur-Containing Compounds
- Chemical Reaction Mechanisms
- Porphyrin and Phthalocyanine Chemistry
- T-cell and B-cell Immunology
- Synthesis and Reactivity of Heterocycles
- Synthesis and Properties of Aromatic Compounds
- Gout, Hyperuricemia, Uric Acid
- Genomic variations and chromosomal abnormalities
- Platelet Disorders and Treatments
- Alcohol Consumption and Health Effects
- Epigenetics and DNA Methylation
Ritsumeikan University
2012-2024
Saigata National Hospital
2005-2016
Noguchi Institute
1989-2006
University of Fukui
1991-2005
National Institute of Allergy and Infectious Diseases
2002
National Institutes of Health
2002
Kyushu University
1988-1999
Kyoto University
1998
Kobe University
1997
Tokyo University of Science
1997
Signaling through the B cell antigen receptor (BCR) results in rapid increases tyrosine phosphorylation on a number of proteins. The BCR associates with two classes kinase: Src-family kinase (Src-protein-tyrosine [PTK]; Lyn, Fyn, Blk, or Lck) and Syk kinase. We have investigated interaction between Src-PTK signaling. In contrast to wild-type cells, BCR-mediated activation its vitro activity were profoundly reduced lyn-negative cells. requirement induce was also demonstrated by cotransfection...
The 75-kD HS1 protein is highly tyrosine-phosphorylated during B cell antigen receptor (BCR)-mediated signaling. Owing to low expression of HS1, WEHI-231-derived M1 cells, unlike the parental are insensitive BCR-mediated apoptosis. Here, we show that BCR-associated tyrosine kinases Lyn and Syk synergistically phosphorylate Tyr378 Tyr-397 critical residues for its BCR-induced phosphorylation. In addition, wild-type a mutant carrying mutations Phe-378 Phe-397 was unable render cells sensitive...
N α-Fmoc(9-fluorenylmethyloxycarbonyl)- β-glycosylated-L-asparagine ester, which is a useful precursor for the synthesis of N-glycopeptide, was obtained in good yield by reaction glycosyl azide and α-Fmoc-L-aspartic acid α-monoester presence triethylphosphine dichloromethane.
We isolated two cDNA clones of rat Hex, a homeobox protein, studied its expression in liver and various cells, characterized the protein. The levels Hex mRNA were only slightly increased rats refed with high-carbohydrate diet or after partial hepatectomy. Whereas was detected hepatocytes from adult also highly differentiated hepatoma no poorly cells. embryo aged 15 days. Expression F9 cells during differentiation into visceral endoderm by treatment retinoic acid. This stimulation occurred...
Recent studies have shown that O-mannosyl glycans are present in several mammalian glycoproteins. Although knowledge on the functional roles of these is accumulating, their biosynthetic pathways poorly understood. Here we report identification and initial characterization a novel enzyme capable forming GlcNAc beta 1-2Man linkage, namely UDP-N-acetylglucosamine: O-linked mannose beta-1,2-N-acetylglucosaminyltransferase microsome fraction newborn rat brains. The transfers to beta-linked...
We have investigated the involvement of protein‐tyrosine kinases in thrombin‐induced aggregation human platelets, using ST638 and genistein which are known inhibitors kinase. Preincubation platelets with 50 μM or 25 μg/ml completely blocked platelet induced 0.05 unit/ml thrombin. The increase phosphorylation bands (135‐, 124‐, 76‐, 64‐, 60‐kDa) thrombin was also inhibited by these a dose‐dependent manner. These aspirin‐treated platelets. Increase intracellular Ca 2+ concentration higher...
A chicken B cell line DT40 and its syk ‐negative or lyn mutants were used to investigate the roles of protein‐tyrosine kinases in oxidant stress signaling. The data presented here for wild‐type cells demonstrate that hydrogen peroxide stimulates p53/56 ‐dependent tyrosine phosphorylation activation p72 , induces a rapid prolonged elevation intracellular calcium, which consists calcium release from stores influx extracellular space. Hydrogen‐peroxide‐triggered mobilization was impaired both...
Genetic studies have revealed that Cbl-b plays a negative role in the antigen receptor–mediated proliferation of lymphocytes. However, we show Cbl-b–deficient DT40 B cells display reduced phospholipase C (PLC)-γ2 activation and Ca2+ mobilization upon cell receptor (BCR) stimulation. In addition, overexpression WEHI-231 mouse resulted augmentation BCR-induced mobilization. interacted with PLC-γ2 helped association Bruton's tyrosine kinase (Btk), as well linker protein (BLNK). was...
To determine the regulatory role of p72syk in lymphocyte activation, peripheral blood lymphocytes were treated with 10 mM hydrogen peroxide. Hydrogen peroxide induced a rapid elevation activity (4-6-fold) and dramatic increase tyrosine phosphorylation number cellular proteins, including phospholipase C gamma 1 (PLC 1) p72syk. Monoclonal antibodies to PLC co-precipitated from peroxide-stimulated cell lysates, but not unstimulated lysates. Furthermore, we observed rise intracellular Ca2+,...
in porcine polymorphonuclear neutrophils
Rett syndrome (RTT), a neurodevelopmental disorder, is mainly caused by mutations in methyl CpG-binding protein 2 (MECP2), which has multiple functions such as binding to methylated DNA or interacting with transcriptional co-repressor complex. It been established that alterations cyclin-dependent kinase-like 5 (CDKL5) forkhead box G1 (FOXG1) correspond distinct disorders, given series of studies have indicated RTT also either one these genes. We investigated the evolution and molecular...
The ability of the lectin concanavalin A (ConA) and N ‐formyl‐methionyl‐leucyl‐phenylalanine (fMLF) to induce protein‐tyrosine phosphorylation in human neutrophils was examined by immunoblot analysis. ConA caused an increase tyrosine protein bands with apparent molecular masses 120, 80, 76, 66 40 kDa; on other hand, fMLF those only 80‐kDa 40‐kDa proteins. These phosphorylations were time‐ dose‐dependent. induced suppressed but that not pertussis toxin pretreatment. At same time, pretreatment...
Induced pluripotent stem cells (iPSCs) offer an invaluable tool for biological research and regenerative medicine. We report establishment of rat iPSCs (riPSCs) using a plasmid vector encoding four transcription factors, Oct3/4, Sox2, c-Myc Klf4. Although all riPSC clones were generated cultured under the same conditions, expressed hallmark pluripotency markers differentiated successfully in vitro, expression keratan sulfate glycan epitope with unique properties defined by R-10G antibody...
Protein-tyrosine kinases and phosphatases play an important role in cytokine-mediated cell growth. The proliferation of a human myeloid leukemia line, AML193, is dependent on interleukin-3 (IL-3) or granulocyte colony-stimulating factor. In the current study, we demonstrated that non-receptor-type protein-tyrosine kinase, Syk, was immediately activated by stimulation with IL-3 factor AML193 cells. We further investigated relation Syk IL-3-mediated signaling found receptor beta subunit...
Journal Article p72syk Is Activated by Vanadate plus H2O2 in Porcine Platelets and Phosphorylates GTPase Activating Protein on Tyrosine Residue(s) Get access Katsuya Nagai, Nagai *Department of Biochemistry, Fukui Medical SchoolMatsuoka, 910–11 Search for other works this author on: Oxford Academic PubMed Google Scholar Tetsuya Inazu, Inazu Hirohei Yamamura 2 910–11†Biosignal Research Center, Kobe UniversityNada-ku, 657 2To whom correspondence should be addressed at: Department School,...
Ovarian granulosa cells undergo cell growth and cytodifferentiation during follicular maturation. In a number of tissues, the gene expression that is responsible for largely dependent on E box(es) located upstream genes. this study, we report cloning cDNA(s) encoding box (5'-CACGTG-3')-binding protein from rat cDNA library using yeast one-hybrid system. When multiple sequences were used as target, obtained positive clone encodes homologue stimulatory factor 2 (USF2). An analysis nucleotide...