A5079222647- Neurogenesis and neuroplasticity mechanisms
- Developmental Biology and Gene Regulation
- Epigenetics and DNA Methylation
- Axon Guidance and Neuronal Signaling
- Pluripotent Stem Cells Research
- Animal Genetics and Reproduction
- Congenital heart defects research
- Hedgehog Signaling Pathway Studies
- MicroRNA in disease regulation
- Genetics and Neurodevelopmental Disorders
- Birth, Development, and Health
- Neuroendocrine regulation and behavior
- Fatty Acid Research and Health
- Olfactory and Sensory Function Studies
- Cleft Lip and Palate Research
- RNA Research and Splicing
- Autism Spectrum Disorder Research
- Genomics and Chromatin Dynamics
- CRISPR and Genetic Engineering
- Genetic and Clinical Aspects of Sex Determination and Chromosomal Abnormalities
- Neuroinflammation and Neurodegeneration Mechanisms
- Diet and metabolism studies
- Infant Health and Development
- Health, Environment, Cognitive Aging
- Mitochondrial Function and Pathology
Tohoku University
2016-2025
Chibune General Hospital
2025
Japan Agency for Medical Research and Development
2019-2021
Tohoku Medical Megabank Organization
2015-2021
The Centers
2004-2019
Fukushima Medical University
2018
Science Council of Japan
2006-2016
Japan Science and Technology Agency
2001-2012
Institute of Developmental Physiology
2011-2012
Science and Technology Corporation (United States)
1999-2010
Abstract The Tohoku Medical Megabank Organization reports the whole-genome sequences of 1,070 healthy Japanese individuals and construction a population reference panel (1KJPN). Here we identify through this high-coverage sequencing (32.4 × on average), 21.2 million, including 12 million novel, single-nucleotide variants (SNVs) at an estimated false discovery rate <1.0%. This detailed analysis detected signatures for purifying selection regulatory elements as well coding regions. We also...
The Great East Japan Earthquake (GEJE) and resulting tsunami of March 11, 2011 gave rise to devastating damage on the Pacific coast Tohoku region. Medical Megabank Project (TMM), which is being conducted by University Organization (ToMMo) Iwate (IMM), has been launched realize creative reconstruction solve medical problems in aftermath this disaster. We started two prospective cohort studies Miyagi Prefectures: a population-based adult study, TMM Community-Based Cohort Study (TMM CommCohort...
The subventricular zone (SVZ) produces different subclasses of olfactory bulb (OB) interneurons throughout life. Little is known about the molecular mechanisms controlling production types interneurons. Here we show that most proliferating adult SVZ progenitors express transcription factor Pax6, but only a small subpopulation migrating neuroblasts and new OB derived from these retains Pax6 expression. To elucidate cell-autonomous role in neurogenesis, transplanted green fluorescent...
Arodent cardiac side population cell fraction formed clonal spheroids in serum-free medium, which expressed nestin, Musashi-1, and multi-drug resistance transporter gene 1, markers of undifferentiated neural precursor cells. These were lost following differentiation, replaced by the expression neuron-, glial-, smooth muscle cell–, or cardiomyocyte-specific proteins. Cardiosphere-derived cells transplanted into chick embryos migrated to truncus arteriosus outflow tract contributed dorsal root...
Hu proteins are mammalian embryonic lethal abnormal visual system (ELAV)-like neuronal RNA-binding that contain three RNA recognition motifs. Although Drosophila ELAV is required for the correct differentiation and survival of neurons, roles played by genes in nervous remain largely unknown. To explore vivo functions mouse proteins, we overexpressed them rat pheochromocytoma PC12 cells, where they induced phenotype absence nerve growth factor. We have characterized various forms mHuB mHuC...
The use of stem cells has enabled the successful generation simple organs. However, anatomically complicated organs such as kidney have proven more refractory to stem-cell-based regenerative techniques. Given limits allogenic organ transplantation, an ultimate therapeutic solution is establish self-organs from autologous and transplant them syngrafts back into donor patients. To this end, we striven in vitro factory build up complex structures adult by using a target organ. Cultivation human...
ABSTRACT Pax-6 is a member of the vertebrate Pax gene family, which structurally related to Drosophila pair-rule gene, paired. In mammals, expressed in several discrete domains developing CNS and has been implicated neural development, although its precise role remains elusive. We found novel Small eye rat strain (rSey2) with phenotypes similar mouse eye. Analyses revealed one base (C) insertion an exon encoding region downstream paired box resulting generation truncated protein due frame...
ABSTRACT Like other tissues and organs in vertebrates, multipotential stem cells serve as the origin of diverse cell types during genesis mammalian central nervous system (CNS). During early development, self-renew increase their total numbers without overt differentiation. At later stages, withdraw from this self-renewal mode, are fated to differentiate into neurons glia a spatially temporally regulated manner. However, molecular mechanisms underlying important step differentiation remain...
Deficits in prepulse inhibition (PPI) are a biological marker for schizophrenia. To unravel the mechanisms that control PPI, we performed quantitative trait loci (QTL) analysis on 1,010 F2 mice derived by crossing C57BL/6 (B6) animals show high PPI with C3H/He (C3) low PPI. We detected six major acoustic startle response, and four latency to response peak, some of which were sex-dependent. A promising candidate Chromosome 10-QTL was Fabp7 (fatty acid binding protein 7, brain), gene...
In neural crest formation, transcription factors, such as group E Sox and Snail1/Snail2 (Slug) regulate subsequent epithelial-mesenchymal transition (EMT) migration. particular, Sox9 has a strong effect on EMT differentiation of crest-derived cartilages in the cranium. It remains unclear, however, how functions these events, activity is regulated. this study, our gain-of-function loss-of-function experiments reveal that essential for BMP signal-mediated induction Snail2 avian crest. We also...
Therapeutic focused-ultrasound to the hippocampus has been reported exert neuroprotective effects on dementia. In present study, we examined whether whole-brain LIPUS (low-intensity pulsed ultrasound) therapy is effective and safe in 2 mouse models of dementia (vascular dementia, VaD Alzheimer's disease, AD), if so, elucidate common underlying mechanism(s) involved.We used bilateral carotid artery stenosis (BCAS) model with micro-coils male C57BL/6 mice as a 5XFAD transgenic an AD model. We...
Background: We established a community-based cohort study to assess the long-term impact of Great East Japan Earthquake on disaster victims and gene-environment interactions incidence major diseases, such as cancer cardiovascular diseases.
New neurons are continually produced after birth from neural stem/progenitor cells (NSCs/NPCs) in the hippocampal dentate gyrus (DG). Recent studies have reported that fatty acid binding protein 7 (Fabp7/brain lipid (BLBP)) is required for maintenance of embryonic NSCs/NPCs and identified an association between Fabp7 gene behavioral paradigms correlate with functions. However, specific roles Fabps postnatal neurogenesis remain unknown. Herein, we demonstrate effects Fabp7, another Fabp,...
Article5 January 2021Open Access Transparent process Paternal age affects offspring via an epigenetic mechanism involving REST/NRSF Kaichi Yoshizaki orcid.org/0000-0002-2540-332X Department of Developmental Neuroscience, Tohoku University Graduate School Medicine, Sendai, Japan Disease Model, Aichi Disability Center, Aichi, Search for more papers by this author Ryuichi Kimura orcid.org/0000-0002-1090-7162 Drug Discovery Kyoto Kyoto, Hisato Kobayashi orcid.org/0000-0003-3800-4691 NODAI Genome...
Neural crest is induced at the junction of epidermal ectoderm and neural plate by mutual interaction these tissues. In previous studies, BMP4 has been shown to pattern ectodermal tissues, can induce cells from plate. this study, we show that epidermally expressed Delta1, which encodes a Notch ligand, required for activation and/or maintenance Bmp4 expression in tissue, thus indirectly induction epidermis-neural boundary. epidermis additionally inhibits formation so generation restricted junction.
Pax6 is a transcription factor with key functional roles in the developing brain. promotes neuronal differentiation via transcriptional regulation of Neurogenin2 ( Ngn2 ) gene, although expression appears proliferating neuroepithelial cells before onset neurogenesis. Here, we identified Fabp7 BLBP/B-FABP ), member fatty acid-binding protein (FABP) family, as downregulated gene embryonic brain mutant rat rSey 2 /rSey by microarray analysis. Marked reduction was confirmed quantitative PCR....
In ventricular cells of the mouse telencephalon, differential expression cadherin cell adhesion molecules defines neighbouring regions; R-cadherin delineates future cerebral cortex, while cadherin-6 lateral ganglionic eminence. By using labelling analyses in whole embryo culture system, we demonstrated that interface between and is a boundary for lineage restriction at embryonic day 10.5. Interestingly, when group with exogenous were generated to straddle cortico-straital by electroporation...