A5005512458- Congenital Heart Disease Studies
- Cardiomyopathy and Myosin Studies
- Cardiac electrophysiology and arrhythmias
- thermodynamics and calorimetric analyses
- Photoreceptor and optogenetics research
- Cardiovascular Function and Risk Factors
- Ion channel regulation and function
- Cardiac Structural Anomalies and Repair
- Cardiovascular Effects of Exercise
- Adipose Tissue and Metabolism
- Neonatal Respiratory Health Research
- Neuroscience and Neural Engineering
- Cardiac Arrhythmias and Treatments
- Coronary Artery Anomalies
- 3D Printing in Biomedical Research
- Cellular Mechanics and Interactions
- Cardiac Valve Diseases and Treatments
- Pulmonary Hypertension Research and Treatments
- Mechanical Circulatory Support Devices
- Congenital Diaphragmatic Hernia Studies
- Vascular anomalies and interventions
- Cardiovascular Conditions and Treatments
- Cardiovascular Issues in Pregnancy
- Microtubule and mitosis dynamics
- Kawasaki Disease and Coronary Complications
National Institutes for Quantum Science and Technology
2019-2025
Iwate Medical University
2015-2024
Jikei University School of Medicine
2012-2024
Waseda University
2011-2022
Takasaki Advanced Radiation Research Institute
2022
Miwa Internal Medicine Clinic
2022
Japan Science and Technology Agency
2019-2022
Tohoku Medical Megabank Organization
2020-2021
Takasaki City University of Economics
2020
Gunma Children's Medical Center
2016
The homeostasis of body temperature and energy balance is one the major principles in biology. Nanoscale thermometry aqueous solutions a challenging but crucial technique to understand molecular basis this essential process. Here, we developed ratiometric nanothermometer (RNT) for intracellular measurement real time. Both thermosensitive fluorophore, β-diketonate chelate europium(III) thenoyltrifluoroacetonate, thermoinsensitive rhodamine 101, which was used as self-reference, are embedded...
Background: We established a community-based cohort study to assess the long-term impact of Great East Japan Earthquake on disaster victims and gene-environment interactions incidence major diseases, such as cancer cardiovascular diseases.
We fabricated fluorescent nanoparticles which monitor temperature changes without sensitivity to pH (4–10) and ionic strength (0–500 mM). The nanothermometers spontaneously enter living HeLa cells via endocytosis, enclosed in acidic organelles, i.e., endosome/lysosome, then transported along microtubules a temperature-dependent manner, working as "walking nanothermometers".
Background: The genetic background of catecholaminergic polymorphic ventricular tachycardia (CPVT) has been extensively investigated for the last decade in Western countries, but it remains unstudied Asian population. Methods and Results: In 50 Japanese probands from unrelated families who satisfied clinical criteria CPVT, testing was conducted all exons on 3 CPVT-related genes: cardiac ryanodine receptor 2 (RYR2), calsequestrin (CASQ2) inward rectifier potassium channel (KCNJ2), features...
Sarcomeric contraction in cardiomyocytes serves as the basis for heart’s pump functions mammals. Although it plays a critical role circulatory system, myocardial sarcomere length (SL) change has not been directly measured vivo under physiological conditions because of technical difficulties. In this study, we developed high speed (100–frames per second), resolution (20-nm) imaging system sarcomeres living mice. Using conducted three-dimensional analysis dynamics left ventricular myocytes...
Intracellular Ca(2+) distribution and its dynamics are essential for various cellular functions. We show with single HeLa cells that a microscopic heat pulse induces uptake into intracellular stores during heating release from them at the onset of recooling, overshoot occurs above critical value temperature change, which decreases 1.5 to 0.2 degrees C on increasing experimental 22 37 C. This highly thermosensitive is probably attributable altered balance between by endoplasmic reticulum...
Nanometry is widely used in biological sciences to analyze the movement of molecules or molecular assemblies cells and vivo. In cardiac muscle, a change sarcomere length (SL) by mere ∼100 nm causes substantial contractility, indicating need for simultaneous measurement SL intracellular Ca(2+) concentration ([Ca(2+)]i) cardiomyocytes at high spatial temporal resolution. To accurately motion individual sarcomeres with nanometer precision during excitation-contraction coupling, we applied...
Polydimethylsiloxane (PDMS) has many desirable features for microfluidics applications, particularly in diagnostics and pharmaceuticals, but its hydrophobicity the lack of a practical method bonding PDMS layers limit use. Moreover, flexibility causes unwanted deformation during use some applications. Here, we report simple solving these problems simultaneously using an electron beam (EB) or γ-rays, which are commonly used sterilizing medical products. Simply by applying EB γ-ray irradiation...
The elasticity, topography, and chemical composition of cell culture substrates influence behavior. However, the cellular responses toin vivoextracellular matrix (ECM), a hydrogel proteins (mainly collagen) polysaccharides, remain unknown as there is no substrate that preserves key features native ECM. This study introduces novel collagen hydrogels can combine reproduce correlation between concentration (C) elastic modulus (E) in A simple reagent-free method based on radiation-cross-linking...
Optical microheating technologies have revealed how biological systems sense heating and cooling at the microscopic scale. Sensing is based on thermosensitive biochemical reactions that frequently engage membrane proteins, Ca2+ channels, pumps to convert sensing information as signaling in cells. These findings highlight feasibility of thermally manipulating intracellular signaling. However, would behave, particularly multicellular systems, remains elusive. In this study, extend ability...
ABSTRACT Silicone rubbers, such as polydimethylsiloxane (PDMS), have been widely used in biotechnology and medical science because of their excellent properties. However, microfabrication silicone rubbers remains to be problematic the high viscosity adhesiveness (tackiness) rubber, making it difficult fill molds reproduce shapes during soft lithography. Also, patterns made rubber are prone pattern collapse. To address these, we designed molecules that can modified by radiation whose hardness...
Type I collagen (Col-I) is the most abundant fibrous protein in extracellular matrix (ECM); its densification involved conditions including cancer, fibrosis, vascular diseases, and aging. Increased Col-I concentrations lead to increased ECM fibril density stiffening; therefore, effects of such changes microenvironment cells require examination. Three types gels were developed by introducing reagent-free radiation-induced crosslinking, while adjusting distance between monomers or fibrils. The...
Abstract Skeletal muscles are classified into slow-twitch composed primarily of type I and IIa fibers with high oxidative metabolism, fast-twitch IIx IIb glycolytic metabolism. Fiber-type shifts occur during development aging; however, the stimuli that shift these types remain unclear. We analyzed role mechanical in myotube formation to characteristics each fiber using crosslinked gelatin gels tunable elasticity (10–230 kPa) microgrooves (3–50 µm). C2C12 myotubes on 10 kPa gel increased...
Background Arrhythmia is a major late complication in adults with repaired tetralogy of Fallot, although large-scale study has not been carried out Japan. Methods and Results A nationwide multicenter 512 operative survivors was performed. Actuarial survival rate at 30 years (maximum follow-up) 98.4%. Fifty-four patients (10.5%) had clinically important arrhythmias, including 23 bradycardia caused by sick sinus syndrome or atrioventricular block (AVB). patient complete AVB (CAVB) without...
This study investigates the effects of prenatal corticosteroid administration on newborn sympathoadrenal mechanisms involved in postnatal adaptation. Randomly assigned preterm (122-125 days) fetal sheep were treated with hydrocortisone or saline for 60 h and delivered by cesarean section. We examined physiological adaptation, responses, cardiac beta-receptor density, receptor-cyclase system. observed increased ventilatory, cardiovascular, metabolic responses function corticosteroid-treated...
Abstract Optical microheating is a powerful non-invasive method for manipulating biological functions such as gene expression, muscle contraction and cell excitation. Here, we demonstrate its potential usage regulating neurite outgrowth. We found that optical with water-absorbable 1,455-nm laser beam triggers directional explosive outgrowth branching in rat hippocampal neurons. The focused under microscope rapidly increases the local temperature from 36 °C to 41 (stabilized within 2 s),...
The persistence of high serum osmolality in the early postnatal period is a risk for developing patent ductus arteriosus (PDA). Early aggressive nutrition (EAN), involving total parenteral (TPN), by which enough concentrations glucose and amino acids are administered intravenously, recommended postnatally to improve neurological prognosis preterm infants. However, effects EAN TPN on development PDA have not been adequately studied.