A5102018353- Enzyme Production and Characterization
- Multiple Myeloma Research and Treatments
- Biofuel production and bioconversion
- Microbial Metabolism and Applications
- Hemoglobinopathies and Related Disorders
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
- Microbial Metabolites in Food Biotechnology
- Nuclear Receptors and Signaling
- Genetic Associations and Epidemiology
- Epigenetics and DNA Methylation
- Microbial Metabolic Engineering and Bioproduction
- Chemokine receptors and signaling
- Health, Environment, Cognitive Aging
- Amino Acid Enzymes and Metabolism
- Genomics and Rare Diseases
- Birth, Development, and Health
- Cytokine Signaling Pathways and Interactions
- RNA modifications and cancer
- Enzyme Catalysis and Immobilization
- Pregnancy and preeclampsia studies
- Microbial Natural Products and Biosynthesis
- Enzyme Structure and Function
- Genomics and Chromatin Dynamics
- Protein Tyrosine Phosphatases
Radiation Effects Research Foundation
2019-2023
Tohoku Medical Megabank Organization
2011-2021
Tohoku University
2013-2021
University of Michigan–Ann Arbor
2005-2017
Michigan United
2014
Tokushima University
2012
Hiroshima University
1989-2008
Northwestern University
2002
Jikei University School of Medicine
1993-2002
Robert H. Lurie Comprehensive Cancer Center of Northwestern University
2002
Abstract The Tohoku Medical Megabank Organization reports the whole-genome sequences of 1,070 healthy Japanese individuals and construction a population reference panel (1KJPN). Here we identify through this high-coverage sequencing (32.4 × on average), 21.2 million, including 12 million novel, single-nucleotide variants (SNVs) at an estimated false discovery rate <1.0%. This detailed analysis detected signatures for purifying selection regulatory elements as well coding regions. We also...
The Great East Japan Earthquake (GEJE) and resulting tsunami of March 11, 2011 gave rise to devastating damage on the Pacific coast Tohoku region. Medical Megabank Project (TMM), which is being conducted by University Organization (ToMMo) Iwate (IMM), has been launched realize creative reconstruction solve medical problems in aftermath this disaster. We started two prospective cohort studies Miyagi Prefectures: a population-based adult study, TMM Community-Based Cohort Study (TMM CommCohort...
The genomic clone of mouse IL-6 was isolated and compared with the human gene. comparison revealed that consists five exons four introns overall organization is similar to gene although third intron about 2 kb longer. sequence similarity in coding region 60%, whereas 3'-untranslated first 300-bp 5'-flanking are highly conserved (greater than 80%). Several blocks high homology also found introns. Furthermore, sequences transcriptional enhancer elements such as c-fos serum responsive element...
The interleukin-6 (IL-6) promoter is rapidly and transiently activated with other cytokines, including IL-1, tumor necrosis factor, platelet-derived growth as well phorbol esters agents that increase intracellular cyclic AMP. In this study, we have investigated cis-acting regulatory elements trans-acting factors responsible for IL-1-induced IL-6 gene expression. Studies on the 5' deletion mutants of human suggested IL-1-responsive element was mapped within region (-180 to -123) which...
Background: We established a community-based cohort study to assess the long-term impact of Great East Japan Earthquake on disaster victims and gene-environment interactions incidence major diseases, such as cancer cardiovascular diseases.
Hereditary motor and sensory neuropathy with proximal dominant involvement (HMSN-P) is an autosomal-dominant neurodegenerative disorder characterized by widespread fasciculations, proximal-predominant muscle weakness, atrophy followed distal involvement. To date, large families affected HMSN-P have been reported from two different regions in Japan. Linkage haplotype analyses of previously new the use high-density SNP arrays further defined minimum candidate region 3.3 Mb chromosomal 3q12....
Significance Sickle cell disease (SCD) is one of the most common inherited disorders. A mutation in β-globin gene causes deformation red blood cells into a sickle shape, which turn intravascular hemolysis and vaso-occlusion resulting damage to multiple organs. Most studies that propose develop new SCD therapies include induction fetal γ-globin expression inhibit formation as their ultimate goal. In contrast, we demonstrate here activation nuclear factor erythroid 2-related 2 (Nrf2)...
Nuclear receptors TR2 and TR4 (TR2/TR4) were previously shown to bind in vitro direct repeat elements the mouse human embryonic fetal β-type globin gene promoters play critical roles silencing of these genes. By chromatin immunoprecipitation (ChIP) we show that, adult erythroid cells, TR2/TR4 but not β-globin promoter. We purified protein complexes containing biotin-tagged from cells identified DNMT1, NuRD, LSD1/CoREST repressor complexes, as well HDAC3 TIF1β, all known confer epigenetic...
Metabolomics is a promising avenue for biomarker discovery. Although the quality of metabolomic analyses, especially global metabolomics (G-Met) using mass spectrometry (MS), largely depends on instrumentation, potential bottlenecks still exist at several basic levels in workflow. Therefore, we established precise protocol initially G-Met analyses human blood plasma to overcome some these difficulties. In our protocol, samples are deproteinized 96-well plate an automated liquid-handling...
In addition to serving as a prosthetic group for enzymes and hemoglobin structural component, heme is crucial homeostatic regulator of erythroid cell development function. While lncRNAs modulate diverse physiological pathological cellular processes, their involvement in heme-dependent mechanisms largely unexplored. this study, we elucidated lncRNA (UCA1)-mediated mechanism that regulates metabolism human cells. We discovered UCA1 expression dynamically regulated during maturation, with...
Testicular receptors 2 and 4 (TR2/4) constitute a subgroup of orphan nuclear that play important roles in spermatogenesis, lipid lipoprotein regulation, the development central nervous system. Currently, little is known about structural features ligand regulation these receptors. Here we report crystal structure ligand-free TR4 binding domain, which reveals an autorepressed conformation. The pocket filled by C-terminal half helix 10, cofactor site occupied AF-2 helix, thus preventing...
Human erythrocyte protein phosphatase 2A, which comprises a 34-kDa catalytic C subunit, 63-kDa regulatory A subunit and 74-kDa B'' (delta) was phosphorylated at serine residues of in vitro by cAMP-dependent kinase (A-kinase). In the presence absence 0.5 microM okadaic acid (OA), A-kinase gave maximal incorporation 1.7 1.0 mol phosphate per B'', respectively. The Km value for CAB'' 0.17 +/- 0.01 OA. major phosphorylation sites were identified as Ser-60, -75 -573 OA, Ser-75 Phosphorylation did...
To globally survey the changes in transcriptional landscape during terminal erythroid differentiation, we performed RNA sequencing (RNA-seq) on primary human CD34+ cells after ex vivo differentiation from earliest into most mature cell stages. This analysis identified thousands of novel intergenic and intronic transcripts as well alternative transcript isoforms. After rigorous data filtering, 51 (presumptive) protein-coding transcripts, 5326 long 679 small non-coding candidates remained. The...
Personalized healthcare (PHC) based on an individual's genetic make-up is one of the most advanced, yet feasible, forms medical care. The Tohoku Medical Megabank (TMM) Project aims to combine population genomics, genetics and prospective cohort studies develop a critical infrastructure for establishment PHC. To date, TMM CommCohort (adult general population) BirThree Cohort (birth+three-generation families) have conducted recruitments baseline surveys. Genome analyses as part will aid in...