A5077124170- Multiple Myeloma Research and Treatments
- RNA modifications and cancer
- RNA Research and Splicing
- RNA and protein synthesis mechanisms
- Chemokine receptors and signaling
- Cytokine Signaling Pathways and Interactions
- Semiconductor materials and devices
- Glycosylation and Glycoproteins Research
- Dialysis and Renal Disease Management
- Advancements in Semiconductor Devices and Circuit Design
- NF-κB Signaling Pathways
- Cell Adhesion Molecules Research
- T-cell and B-cell Immunology
- Renal Diseases and Glomerulopathies
- Cancer-related molecular mechanisms research
- Peptidase Inhibition and Analysis
- Monoclonal and Polyclonal Antibodies Research
- Immune Cell Function and Interaction
- interferon and immune responses
- PI3K/AKT/mTOR signaling in cancer
- Neurogenetic and Muscular Disorders Research
- Silicon Carbide Semiconductor Technologies
- Systemic Sclerosis and Related Diseases
- Chronic Kidney Disease and Diabetes
- Gastrointestinal Tumor Research and Treatment
Nagoya Ekisaikai Hospital
2021-2025
Konan Kosei Hospital
2017-2022
Tokyo University of Agriculture and Technology
2009-2019
Toyokawa City Hospital
2019
Kyoto first Red Cross hospital
2019
Japanese Red Cross Nagoya Daiichi Hospital
2018
Hyogo Medical University
1986-2017
Japan Science and Technology Agency
2011-2016
Tokai Central Hospital
2013-2015
Edge Technologies (United States)
2011
In this study, we demonstrated that IL-6 was a possible autocrine growth factor for rat mesangial cells (MC). rIL-6 induced in vitro of MC at concentration 2 to 200 ng/ml and activity found the supernatant cultured MC. Northern blot analysis as well situ hybridization revealed mRNA expressed Of urine samples from patients with proliferative glomerulonephritis (PGN) 50% were contain significant (ranging 30 126 pg/ml). Urine other type primary glomerular diseases such minimal change nephrotic...
During ribosome biogenesis, ribosomal RNAs acquire various chemical modifications that ensure the fidelity of translation, and dysregulation modification processes can cause proteome changes as observed in cancer inherited human disorders. Here, we report complete all 80S determined with quantitative mass spectrometry. We assigned 228 sites 14 different post-transcriptional modifications, most which are located functional regions ribosome. All detected typical eukaryotic RNAs, no...
We present the complete chemical structures of rRNAs from eukaryotic model organism, Saccharomyces cerevisiae. The final structures, as determined with mass spectrometry-based methodology that includes a stable isotope-labelled, non-modified reference RNA, contain 112 sites 12 different post-transcriptional modifications, including previously unidentified pseudouridine at position 2345 in 25S rRNA. Quantitative stoichiometric analysis modifications each site indicated 94 were almost fully...
Extracellular vesicle (EV) microRNAs are of major interest as potential diagnostic biomarkers in all cancer types. This study aims to identify miRNA profiles shed microvesicles (sMVs) and exosomes (Exos) secreted from the isogenic colorectal (CRC) cell lines SW480 SW620 evaluate their ability predict CRC. Deep sequencing miRNAs parental lysates (CLs) highly-purified sMVs Exos was performed. We focused on enriched EVs dysregulated metastatic cells (SW620) relative primary (SW480)....
The nfkb2 gene encodes the p100 precursor which produces p52 protein after proteolytic cleavage of its COOH-terminal domain. Although product can act as an alternative subunit NF-kappaB, is believed to function inhibitor Rel/NF-kappaB activity by cytoplasmic retention complexes, like other members IkappaB family. However, physiological relevance molecule has not been understood. To assess role in vivo, we generated, targeting, mice lacking but still containing a functional protein. Mice with...
The polypeptide (p)50 molecule, a subunit of nuclear factor (NF)-kappaB, is produced after proteolytic processing the p105 precursor (NF-kappaB1). Although has been postulated to play role in regulation Rel/NF-kappaB activity, its physiological relevance remains unclear. To investigate that, we generated mutant mice lacking COOH terminal half precursor, but expressing p50 product (p105-/-). These displayed an inflammatory phenotype composed lymphocytic infiltration lungs and liver, increased...
Abstract Previously we reported that LIM1863 colorectal cancer (CRC) cells secrete three distinct extracellular vesicle subtypes – two subpopulations of exosomes (apical EpCAM-Exos and basolateral A33-Exos) shed microvesicles (sMVs) with protein miRNA signatures. Here, extend our omics approach to understand the fundamental role LIM1863-derived EVs by performing a comprehensive analysis their mRNAs long non-coding RNAs (lncRNAs) using RNA-Seq. We show 2,389 mRNAs, 317 pseudogene transcripts,...
In cytoplasm, the survival of motor neuron (SMN) complex delivers pre-small nuclear RNAs (pre-snRNAs) to heptameric Sm ring for assembly on pre-snRNAs at conserved site [A(U) 4–6 G]. Gemin5, a WD40 protein component SMN complex, is responsible recognizing pre-snRNAs. addition, Gemin5 has been reported specifically bind m 7 G cap. this study, we show that domain both necessary and sufficient binding by isothermal titration calorimetry (ITC) mutagenesis assays. We further determined crystal...
Rel family proteins regulate the expression of genes linked to kappa B-binding motifs. Little is known, however, mechanism by which they enhance transcription. We have investigated ability v-Rel and c-Rel oncoproteins interact with components basal transcription machinery. Here we report that both acidic activation domain mapping unique C terminus chicken F9 cell-specific region common TATA-binding protein (TBP) factor IIB (TFIIB) in vitro vivo. also demonstrate TPB interaction regions leads...
We present here a method to correlate tandem mass spectra of sample RNA nucleolytic fragments with an nucleotide sequence in DNA/RNA database, thereby allowing spectrometry (MS/MS)-based identification biological samples. Ariadne, unique web-based database search engine, identifies by two probability-based evaluation steps MS/MS data. In the first step, software evaluates matches between masses product ions generated RNase digest and those calculated from candidate which then predicts...
The 43-kDa trans-activating response region DNA-binding protein 43 (TDP-43) is a product of causative gene for amyotrophic lateral sclerosis (ALS). Despite accumulating evidence that mitochondrial dysfunction underlies the pathogenesis TDP-43-related ALS, roles wild-type TDP-43 in mitochondria are unknown. Here, we show small population present binds directly to subset tRNAs and precursor RNA encoded L-strand mtDNA. Upregulated expression stabilised processing intermediates polycistronic...
We describe here a mass spectrometry (MS)-based analytical platform of RNA, which combines direct nano-flow reversed-phase liquid chromatography (RPLC) on spray tip column and high-resolution LTQ-Orbitrap spectrometer. Operating RPLC under very low flow rate with volatile solvents MS in the negative mode, we could estimate highly accurate values sufficient to predict nucleotide composition approximately 21-nucleotide small interfering detect post-transcriptional modifications yeast tRNA,...
This paper presents the performance of a buried channel MOSFET (BC-MOSFET) that uses bulk region as conducting in contrast with surface conventional device. Normally-off characteristic has been realized p-type silicon gate and ion-implanted n-channel layer. Fabricated short BC-MOSFETs lengths 1-3 µ have shown small shift threshold voltage changing length or drain bias. These devices also high carrier mobility 750 cm <sup xmlns:mml="http://www.w3.org/1998/Math/MathML"...