A5055649452- X-ray Diffraction in Crystallography
- Crystallization and Solubility Studies
- Immune Cell Function and Interaction
- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Pluripotent Stem Cells Research
- Monoclonal and Polyclonal Antibodies Research
- Sarcoma Diagnosis and Treatment
- Tryptophan and brain disorders
- Neuroinflammation and Neurodegeneration Mechanisms
- Neuroscience and Neuropharmacology Research
- Stress Responses and Cortisol
- Endoplasmic Reticulum Stress and Disease
- Immune cells in cancer
- Telomeres, Telomerase, and Senescence
- Genomics and Chromatin Dynamics
- Nitric Oxide and Endothelin Effects
- Epigenetics and DNA Methylation
- Cell Adhesion Molecules Research
- Glycosylation and Glycoproteins Research
- Bone Tumor Diagnosis and Treatments
- CRISPR and Genetic Engineering
- Cancer-related Molecular Pathways
- Genetics, Aging, and Longevity in Model Organisms
Nagoya Women's University
2016-2023
Shubun University
2020-2023
Saitama University
2020
National Hospital Organization
2014-2019
Matsumoto City Hospital
2014-2019
Toho University Medical Center Sakura Hospital
2018
Toho University
2018
Toho University Omori Medical Center
2018
Shinshu University
2005-2017
Matsumoto University
2017
The establishment of human induced pluripotent stem cells (hiPSCs) has enabled the production in vitro, patient-specific cell models disease. In vitro recreation disease pathology from patient-derived hiPSCs depends on efficient differentiation protocols producing relevant adult types. However, myogenic faced obstacles, namely, low efficiency and/or poor reproducibility. Here, we report rapid, efficient, and reproducible into mature myocytes. We demonstrated that inducible expression...
Forkhead transcription factor, DAF-16, regulates genes that contribute both to longevity and resistance various stresses in C. elegans. We others have reported members of the FOXO, mammalian homologs also regulate related stress resistance, such as GADD45. The NAD-dependent protein deacetylase, SIR2, is required for life span extension yeast induced by caloric restriction, which increases a wide variety other organisms, including mammals. Sir2.1, homolog extends acting DAF-16 signaling...
Abstract We recently identified CD8+CD122+ regulatory T cells that directly control CD8+ and CD4+ without intervention of APCs. In this study, we investigated the effector mechanism by using an in vitro regulation system. The profile cytokine expression revealed IL-10 was predominantly produced cells, whereas other cytokines were similarly expressed CD8+CD122− cells. Suppression both proliferation IFN-γ production blocked adding anti-IL-10 Ab to culture but not anti-TGF-β Ab. When removed...
GADD34 is a protein that induced by stresses such as DNA damage. The function of mammalian has been proposed in vitro transfection, but its vivo not yet elucidated. Here we generated and analyzed knockout mice. Despite their embryonic stage- tissue-specific expressions, mice showed no abnormalities at fetal development early adult life. However, GADD34-/- mouse fibroblasts (MEFs), recovery from shutoff synthesis was delayed when MEFs were exposed to endoplasmic reticulum (ER) stress....
We surveyed the clinical symptoms and radiologic features of ancient schwannoma, a rare variant schwannoma characterized by degenerative changes.We present clinical, radiologic, pathologic in seven patients with (mean age, 62 years; range, 45-80 years) treated at our department between 1998 2003.The most characteristic were sign like Tinel's long interval onset surgery interval, 8.3 years). Ancient schwannomas can grow large; biggest tumor seen study was 14 cm long. The highly accurate...
Abstract Experimental autoimmune encephalomyelitis (EAE) is one of the best-documented animal models disease. We examined role CD8+CD122+ regulatory T cells, which we previously identified as naturally occurring cells that effectively regulate CD8+ in EAE. Depletion by vivo administration anti-CD122 mAb resulted persistent EAE symptoms. Transfer into mice at peak score clearly improved symptoms, indicating an important recovery phase This was further confirmed increase and a decrease number...
We have reported that histone acetylation induced by trichostatin A (TSA) promotes p21 <sup>waf1/cip1</sup> (p21) expression, the GC-box located just upstream of TATA box was responsible for TSA-induced promoter activation, and both Sp1 Sp3 were working activator this GC-box. To understand molecular pathway from to family factors-mediated we investigated function p300, one acetyltransferase, in present work. The evidence supporting linkage between p300 activation realized following findings:...
Plasminogen activator inhibitor-1 (PAI-1) is one of the primary inhibitors fibrinolytic system and has been implicated in a variety thrombotic disorders. In this report, stress-induced changes murine PAI-1 gene expression were investigated to study role inhibitor development hypercoagulability. Restraint stress led dramatic induction plasma antigen tissue mRNA with maximum adipose tissues. situ hybridization analysis stressed mice revealed that strong signals for localized hepatocytes, renal...
Abstract In Parkinson's disease, characteristic pathological features are the cell death of nigrostriatal dopamine neurons and formation Lewy bodies composed oxidized proteins. Mitochondrial dysfunction aggregation abnormal proteins have been proposed to cause changes. However, relation between these two factors remains be clarified. this study, effects mitochondrial on oxidative modification accumulation were analyzed using an inhibitor complex I, rotenone, antibodies against acrolein‐...
Histone deacetylase inhibitor-induced expression of p21WAF1 is p53 independent. In the present study, we provide evidence that trichostatin A (TSA), a specific inhibitor histone deacetylase, can elevate H3 and H4 acetylation in NIH3T3 cells at first. To identify transcription factor which responsible for understand potential events occurred during this process, analyze response mouse promoter to TSA detail. The region responsive treatment p21 located -100 bp upstream from initiation site...
Apigenin, a naturally occurring, non-mutagenic flavonoid, has been shown to inhibit UV-induced skin tumorigenesis in mice when topically applied. In this report we have used the mouse keratinocyte 308 cell line, which contains wild-type p53 gene, study effect of apigenin treatment on protein levels and expression its downstream partner, p21/waf1. Cells were treated with 70 μM for various times p21/waf1 assessed by western blot analysis. The level was induced 27-fold after 4 h remained...
CD8+CD122+ regulatory T cells (CD8+CD122+ Treg) are naturally occurring Treg that effectively suppress the proliferation and IFN-γ production of both CD8+ CD4+ target cells. This study investigated molecular mechanisms recognition by using an in vitro culture system reconstitutes action these Naive co-cultured with pre-activated became active produced IL-10 suppressed from syngeneic mouse strains but not allogeneic incompatible MHC. By MHC-congeneic strains, MHC-restricted suppression was...
Abstract The importance of CD8 + CD122 Treg in the maintenance immune homeostasis has been previously demonstrated mice. Because expression pattern and humans is different from that mice, human correspond to murine have not identified. In this study, we performed DNA microarray analyses compare gene profiles cells − mice found CXCR3 was preferentially expressed cells. When analyzed cells, observed a definite population showed similar regulatory activities by vivo vitro assays. While are...
Abstract We identified CD8+CD122+ regulatory T cells (Tregs) and demonstrated their importance in the maintenance of immune homeostasis recovery from experimental autoimmune encephalomyelitis. In this paper, we show that Tregs effectively prevent cure colitis a mouse model. our experiments, was induced lymphocyte-deficient RAG-2−/− mice by transferring CD4+CD45RBhigh were excluded with CD4+ Tregs. Cotransfer clearly suppressed development colitis, suppressive effect similar to CD4+CD45RBlow...
Angiogenic cell therapy represents a novel strategy for ischemic diseases, but some patients show poor responses. We investigated the therapeutic potential of an induced pluripotent stem (iPS) sheet created by magnetite tissue engineering technology (Mag-TE) reparative angiogenesis. Mouse iPS cell-derived Flk-1+ cells were incubated with magnetic nanoparticle-containing liposomes (MCLs). MCL-labeled mixed diluted extracellular matrix (ECM) precursor and magnet was placed on reverse side....
Objectives: To confirm the accuracy and precision of transcutaneous bilirubin (TcB) values measured by a new device with two optical paths (JM-103) value total serum (TSB) level in clinical units measurement. Methods: For comparison levels JM-103 old (JM-102), samples were collected from 77 Japanese infants three different hospitals including 24 preterm 53 term infants. Measurement TcB JM- 102 performed on forehead each infant within 30 min before or after blood sampling. Results: The range...