Abstract We recently identified CD8+CD122+ regulatory T cells that directly control CD8+ and CD4+ without intervention of APCs. In this study, we investigated the effector mechanism by using an in vitro regulation system. The profile cytokine expression revealed IL-10 was predominantly produced cells, whereas other cytokines were similarly expressed CD8+CD122− cells. Suppression both proliferation IFN-γ production blocked adding anti-IL-10 Ab to culture but not anti-TGF-β Ab. When removed...

Background: Caspase-1 (reflects NOD-like receptor protein 3 inflammasomes activity), transforming growth factor-β (TGF-β), and Galectin-3 play significant role in post-AMI fibrosis inflammation. Recently, colchicine was shown to dampen inflammation after AMI, however, its direct benefit remains controversial. Objectives. This study aimed analyze the of reducing NT-proBNP, Caspase-1, TGF-β expression improving echocardiography parameter among AMI patients. Methods....

Colorectal cancer is the second most common which caused large number of deaths worldwide. It has been widely known that its undergoing therapies were related to cell metastasis and drug resistance. In line with endeavor reduce side effects those therapies, bio-fabricated silver nanoparticles (AgNPs), are synthesized using plant extracts, massively explored due their potential as anticancer agents. Specific analytical techniques used characterize MOLP-AgNPs, such UV–visible spectrophotometer...

Abstract The importance of CD8 + CD122 Treg in the maintenance immune homeostasis has been previously demonstrated mice. Because expression pattern and humans is different from that mice, human correspond to murine have not identified. In this study, we performed DNA microarray analyses compare gene profiles cells − mice found CXCR3 was preferentially expressed cells. When analyzed cells, observed a definite population showed similar regulatory activities by vivo vitro assays. While are...

Abstract We identified CD8+CD122+ regulatory T cells (Tregs) and demonstrated their importance in the maintenance of immune homeostasis recovery from experimental autoimmune encephalomyelitis. In this paper, we show that Tregs effectively prevent cure colitis a mouse model. our experiments, was induced lymphocyte-deficient RAG-2−/− mice by transferring CD4+CD45RBhigh were excluded with CD4+ Tregs. Cotransfer clearly suppressed development colitis, suppressive effect similar to CD4+CD45RBlow...

While the beneficial effects of colchicine on inflammation and infarcted myocardium have been documented, its impact cardiac fibroblast activation in context MI remains unknown. This study aimed to investigate effect regulation NLRP3 inflammasome IL-1β expression fibroblasts. The 3T3 fibroblasts were exposed 600 μM CoCl2 for 24 hours simulate hypoxia, with normoxic cells as controls. Colchicine (1 μM) was administered hours. ASC-NLRP3 colocalization evaluated using immunofluorescence flow...

Background: Caspase-1 (reflects NOD-like receptor protein 3 inflammasome activity), transforming growth factor-β (TGF-β), and Galectin-3 play significant roles in post-AMI fibrosis inflammation. Recently, colchicine was shown to dampen inflammation after AMI; however, its direct benefit remains controversial. Objectives: This study aimed analyze the of reducing NT-proBNP, Caspase-1, TGF-β,and expression improving systolic-diastolic echocardiography parameters among AMI patients. Methods: A...

While the beneficial effects of colchicine on inflammation and infarcted myocardium have been documented, its impact cardiac fibroblast activation in context myocardial infarction (MI) remains unknown. This study aimed to investigate effect regulation NOD-like receptor family, pyrin domain containing 3 (NLRP3) inflammasome Interleukin-1β (IL-1β) expression fibroblasts. 3T3 fibroblasts were exposed 600 μM CoCl2 for 24 h simulate hypoxia, with normoxic cells as controls. Colchicine (1 μM) was...

Context: Matrix metalloproteinase-2 (MMP-2) is a protein found in tumor cells and important breast cancer spread development. Natural substances like quercetin, cardenolide, epicatechin, coumarin, anthraquinone have been studied for their ability to block MMP-2 activity, which may lead lower invasiveness reduced spread. These bioactive compounds present potential treatment options targeting slowing down the progress of cancer. Aims: To determine mechanism inhibition by active Sterculia...

Context: Breast cancer is characterized by the abnormal growth of cells in ducts and lobules breast. Numerous therapeutic targets for breast are currently under investigation, one which matrix metalloproteinase-2 (MMP-2), a heterodimeric protein highly expressed circulating tumor from patients with pancreatic carcinoma, positioning it as potential target therapy. Anredera cordifolia plant known its medicinal properties anti-cancer effects, particularly cancer. Aims: To determine compounds...

The balance between T helper 17 (Th17) and regulatory cells (Treg) is a new paradigm in the pathogenesis of systemic lupus erythematosus (SLE). Currently, there are no drugs that able to modulate Th17/Treg specifically SLE. Curcumin bioactive agent has specific action against hyperproliferative cells. However, its role modulating SLE still unknown. This research aimed investigate curcumin on CD4+ cell cultures patients.CD4+ from 6 untreated patients healthy subjects were collected,...

Indonesian mistletoe grows on various trees. Mango Mistletoes (Dendrophthoe pentandra) is one type of that grown mango tree (.benalu mangga in bahasa Indonesia). Our study used as a parasitic plant has been for traditional medicine. It known Dendrophtoe pentandra extract (DPE) anti-inflammatory and anticancer. Furthermore, it necessary to follow-up vivo evaluate the response treatment new cancer therapeutic agents. This research aimed determine levels IL-22, myeloperoxide (MPO),...

During Plasmodium berghei (P. berghei) infection, infected erythrocytes are sequestered in gut tissues through microvascular circulation, leading to dysbiosis. This study aimed investigate the effect of Lactobacillus casei (L. casei) and Bifidobacterium longum (B. longum) administration on parasitemia level, microbiota composition, expression cluster differentiation 103 (CD103) intestinal dendritic T regulatory cells (T reg), plasma interferon gamma (IFN-γ) tumor necrosis factor (TNF-α)...

Abstract Naive T cells do not proliferate but remain alive in vivo . In contrast, naive rapidly die an vitro culture, suggesting that some factors are present at the sites of cell circulation missing bovine serum‐containing culture medium, necessary for their survival. The study was designed to search such factors. By functional screening cDNA library from murine lymph node‐derived stromal (LNS) effectively support survival cells, we found nascent polypeptide‐associated complex (α−NAC)...

Introduction. Immunotherapy has been widely used in the treatment of various malignancies with satisfactory results. One agents for immunotherapy is an inhibitor programmed cell death-1 and its ligands (PD-1 PD-L1). However, attempts at utilizing PD-1/PD-L1 osteosarcoma have not yielded favorable This may be due to differences PD-L1 regulation immune landscape osteosarcoma, as mechanism still poorly understood. Therefore, elucidating paramount order improve results using immunotherapy....

Mango mistletoes Dendrophthoe pentandra (MMDP) extract has attracted interest due to its pharmacological properties, including gastro protective effects. The aim of this study was investigate whether MMDP could increase Foxp3 regulatory T cells and inhibits development Th17 cells.Colitis induced in Balb/c mice by rectal administration 2,4,6-trinitrobenzene sulfonic acid (TNBS). were randomly divided into five groups comprising group1 receiving vehicle (the negative control), group 2-5 TNBS,...

Meta-analysis of GWAS in East Asian populations had established 10 loci that were associated with type 2 diabetes. Eight them genome-wide significance and two a border line association. Since these data have not been studied an independent Han Chinese population, we aimed to investigate the association susceptibility diabetes population. We executed case-control study 000 by SNPscan method. Firstly, repetitive sequences assessed. Next, investigated 8 SNPs out constructed GRS those SNPs....

Triple-negative breast cancer (TNBC) tends to be aggressive and metastatic, characteristics attributable its cellular migration capabilities. Afzelin is a chemical compound with anti-metastatic potentials. This study aimed predict proteins involved in TNBC cell which could inhibited by afzelin.The protein database was constructed from the Kyoto Encyclopedia of Genes Genomes pathways collection related motility, then screened for druggability using SuperTarget Therapeutic Target Database. The...

Context: Colorectal cancer (CRC) is a major global health concern as innovative therapeutic strategies to enhance treatment outcomes. This study investigates the potential of ethanol extract derived from Volvariella volvacea in combination with cetuximab for CRC. Aims: To evaluate inhibition topoisomerase II alpha (TOP2A) and include peroxisome proliferator-activated receptor gamma (PPARγ), key regulator cell growth differentiation. Methods: The method used computational modeling molecular...