A5011330479- Pluripotent Stem Cells Research
- Lysosomal Storage Disorders Research
- CRISPR and Genetic Engineering
- Carbohydrate Chemistry and Synthesis
- Acute Myeloid Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Mesenchymal stem cell research
- T-cell and B-cell Immunology
- Acute Lymphoblastic Leukemia research
- Autoimmune and Inflammatory Disorders Research
- Glycosylation and Glycoproteins Research
- Renal and related cancers
- 3D Printing in Biomedical Research
- Epigenetics and DNA Methylation
- Genetics and Neurodevelopmental Disorders
- Immune Cell Function and Interaction
- MicroRNA in disease regulation
- Tissue Engineering and Regenerative Medicine
- RNA Interference and Gene Delivery
- Autophagy in Disease and Therapy
- Zebrafish Biomedical Research Applications
- Erythrocyte Function and Pathophysiology
- Sphingolipid Metabolism and Signaling
- Nuclear Receptors and Signaling
- Neurogenetic and Muscular Disorders Research
Kumamoto University
2015-2024
Fukushima Medical University
2020
RIKEN Center for Computational Science
2004-2009
Organogenesis (United States)
2009
RIKEN
2008
Teradata (United Kingdom)
2008
Howard Hughes Medical Institute
2000-2003
Osaka University
1996-2000
University of California, Los Angeles
2000
Hôpital Saint-Louis
2000
Bipotent mesendoderm that can give rise to both endoderm and mesoderm is an established entity from C. elegans zebrafish. Although previous studies in mouse embryo indicated the presence of bi-potent cells organizer region, characterization its differentiation processes are still unclear. As implicated as major precursor definitive endoderm, identification also essential for exploring endoderm. In this study, we have embryonic stem (ES) cell lines carry GFP gene goosecoid (Gsc) locus...
The embryonic subventricular zone (SVZ) is a critical site for generating cortical projection neurons; however, molecular mechanisms regulating neurogenesis specifically in the SVZ are largely unknown. transcription factor Eomes/Tbr2 transiently expressed progenitor cells. Here we demonstrate that conditional inactivation of Tbr2 during early brain development causes microcephaly and severe behavioral deficits. In mutants number cells reduced differentiation upper layer neurons disturbed....
Analysis of ALS patient iPSC-derived motor neurons indicates that Src/c-Abl inhibitors may have potential for treating ALS.
The establishment of human induced pluripotent stem cells (hiPSCs) has enabled the production in vitro, patient-specific cell models disease. In vitro recreation disease pathology from patient-derived hiPSCs depends on efficient differentiation protocols producing relevant adult types. However, myogenic faced obstacles, namely, low efficiency and/or poor reproducibility. Here, we report rapid, efficient, and reproducible into mature myocytes. We demonstrated that inducible expression...
Mutations in the NPHS1 gene, which encodes NEPHRIN, cause congenital nephrotic syndrome, resulting from impaired slit diaphragm (SD) formation glomerular podocytes. However, methods for SD reconstitution have been unavailable, thereby limiting studies field. In present study, we established human induced pluripotent stem cells (iPSCs) a patient with an missense mutation, and reproduced process using iPSC-derived kidney organoids. The mutant NEPHRIN failed to become localized on cell surface...
P210 Bcr-Abl is an activated tyrosine kinase oncogene encoded by the Philadelphia chromosome associated with human chronic myelogenous leukemia (CML). The disease represents a clonal disorder arising in pluripotent hematopoietic stem cell. During phase, patients present dramatic expansion of myeloid cells and mild anemia. Retroviral gene transfer transgenic expression rodents have demonstrated ability to induce various types leukemia. However, study CML or rodent models has not determined...
Abstract Endothelial cells (ECs) are thought to be derived mainly from the vascular endothelial growth factor receptor 2 (VEGFR-2)+ lateral mesoderm during early embryogenesis. In this study, we specified several pathways for EC differentiation using a murine embryonic stem (ES) cell culture system that is model cellular processes Based on results of in vitro fate analysis, show that, main pathway, committed ECs differentiated through VEGFR-2+ platelet-derived α (PDGFR-α)− single-positive...
Niemann-Pick disease type C (NPC) is a lysosomal storage characterized by abnormal accumulation of free cholesterol and glycolipids. Here, we established induced pluripotent stem cell (iPSC) lines from NPC patients. Hepatocyte-like cells (HLCs) neural progenitors derived the iPSC accumulated displayed impaired autophagy ATP production. A molecular signature related to lipid metabolism was also in NPC-iPSC-derived HLCs. These findings indicate that iPSC-derived can phenocopy human NPC. We...
Fibrodysplasia ossificans progressiva (FOP) is a rare congenital disorder characterized by progressive ossification of soft tissues. FOP caused mutations in activin receptor-like kinase 2 (ALK2) that cause its constitutive activation and result dysregulation BMP signaling. Here, we show generation induced pluripotent stem cells (iPSCs) from FOP-derived skin fibroblasts repressed because incomplete reprogramming inhibition iPSC maintenance. This repression was mostly overcome specific...
Induced pluripotent stem cells (iPSCs) are potentially valuable cell sources for disease models and future therapeutic applications; however, inefficient generation the presence of integrated transgenes remain as problems limiting their current use. Here, we developed a new Sendai virus vector, TS12KOS, which has improved efficiency, does not integrate into cellular DNA, can be easily eliminated. TS12KOS carries KLF4, OCT3/4, SOX2 in single vector generate iPSCs from human blood cells. Using...
ABSTRACT Mesenchymal stem cells (MSCs) are somatic that can be derived from adult bone marrow (BM) and white adipose tissue (WAT), display multipotency self-renewal capacity. Although MSCs essential for formation have already been used in clinical therapy, the origins markers of these remain unknown. In this study, we first investigated developmental process mouse embryos using gene encoding platelet-derived growth factor receptor α (Pdgfra) as a marker. We then traced expressing Pdgfra...
Mitochondria participate in various metabolic pathways, and their dysregulation results multiple disorders, including aging-related diseases. However, the changes mechanisms of mitochondrial disorders are not fully understood. Here, we found that induced pluripotent stem cells (iPSCs) from a patient with myopathy, encephalopathy, lactic acidosis, stroke-like episodes (MELAS) showed attenuated proliferation survival when glycolysis was inhibited. These deficits were rescued by taurine...
The effects of Notch signals on the erythroid/megakaryocytic differentiation hematopoietic cells were examined. Activation by intracellular Notch1 or an estradiol-inducible form Notch1/ER suppressed expression erythroid marker glycophorin A in cell line K562. Although Mock-transfected K562 underwent megakaryocytic response to 12-O-tetradecanoylphorbol-13-acetate (TPA), estradiol-activated induced apoptosis during TPA treatment transfectant, which was accompanied reduced antiapoptotic...
Although there is a criticism that embryonic stem (ES) cell differentiation does not always reflect the process involved in mouse development, it suitable model system to dissect specific pathway. We established culture conditions selectively differentiated ES cells into three germ layers containing mesendoderm, definitive endoderm (DE), visceral (VE), mesoderm, and neuroectoderm. However, molecular mechanisms of under each condition still remain unclear. Here, combination with RNA...
Rest (RE1-silencing transcription factor, also called Nrsf) is involved in the maintenance of undifferentiated state neuronal stem/progenitor cells vitro by preventing precocious expression genes. However, function during neurogenesis vivo remains to be elucidated because early embryonic lethal phenotype conventional knockout mice. In present study, we have generated conditional mice, which allow effect genetic ablation examined vivo. We show that plays a role suppressing genes cultured...
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