Saori Suzuki
A5031472173- SARS-CoV-2 and COVID-19 Research
- Immune Cell Function and Interaction
- Influenza Virus Research Studies
- COVID-19 Clinical Research Studies
- Hepatitis B Virus Studies
- Viral gastroenteritis research and epidemiology
- Immune Response and Inflammation
- T-cell and Retrovirus Studies
- Hepatitis C virus research
- Animal Virus Infections Studies
- SARS-CoV-2 detection and testing
- Immunotherapy and Immune Responses
- interferon and immune responses
- T-cell and B-cell Immunology
- Respiratory viral infections research
- Erythrocyte Function and Pathophysiology
- Wastewater Treatment and Nitrogen Removal
- Chemical Reactions and Isotopes
- Bacillus and Francisella bacterial research
- Blood groups and transfusion
- CRISPR and Genetic Engineering
- Membrane Separation Technologies
- RNA Research and Splicing
- Animal Disease Management and Epidemiology
- Circadian rhythm and melatonin
Hokkaido University
2011-2025
Princeton University
2020-2024
Shiga University of Medical Science
2019-2022
The University of Tokyo
2006-2019
Graduate School USA
2018
Suzuka University of Medical Science
2017
Kyoto University
2013-2016
Nippon Steel (Japan)
2010-2012
Toyohashi University of Technology
2007-2011
Yamagata University
2010-2011
In late 2022, SARS-CoV-2 Omicron subvariants have become highly diversified, and XBB is spreading rapidly around the world. Our phylogenetic analyses suggested that emerged through recombination of two cocirculating BA.2 lineages, BJ.1 BM.1.1.1 (a progeny BA.2.75), during summer 2022. XBB.1 variant most profoundly resistant to BA.2/5 breakthrough infection sera date more fusogenic than BA.2.75. The breakpoint located in receptor-binding domain spike, each region recombinant spike confers...
Many enveloped viruses induce multinucleated cells (syncytia), reflective of membrane fusion events caused by the same machinery that underlies viral entry. These syncytia are thought to facilitate replication and evasion host immune response. Here, we report co-culture human expressing receptor ACE2 with SARS-CoV-2 spike, results in synapse-like intercellular contacts initiate cell-cell fusion, producing resembling those identify lungs COVID-19 patients. To assess mechanism...
In late 2022, various Omicron subvariants emerged and cocirculated worldwide. These variants convergently acquired amino acid substitutions at critical residues in the spike protein, including R346, K444, L452, N460, F486. Here, we characterize convergent evolution of properties one recent lineage concern, BQ.1.1. Our phylogenetic analysis suggests that these five are recurrently acquired, particularly younger lineages. Epidemic dynamics modelling increase viral fitness, a large proportion...
Abstract Circulation of SARS-CoV-2 Omicron XBB has resulted in the emergence XBB.1.5, a new Variant Interest. Our phylogenetic analysis suggests that XBB.1.5 evolved from XBB.1 by acquiring S486P spike (S) mutation, subsequent to acquisition nonsense mutation ORF8. Neutralization assays showed similar abilities immune escape between and XBB.1. We determine structural basis for interaction human ACE2 S protein showing overall structures proteins XBB.1.5. provide intrinsic pathogenicity...
Abstract The unremitting emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) variants necessitates ongoing control measures. Given its rapid spread, the new Omicron subvariant BA.5 requires urgent characterization. Here, we comprehensively analyzed with other BA.1, BA.2, and ancestral B.1.1. Although in vitro growth kinetics was comparable among subvariants, much more fusogenic than BA.1 BA.2. Airway-on-a-chip analysis showed that, had enhanced ability to disrupt...
Abstract In late 2022, the SARS-CoV-2 Omicron subvariants have highly diversified, and XBB is spreading rapidly around world. Our phylogenetic analyses suggested that emerged by recombination of two co-circulating BA.2 lineages, BJ.1 BM.1.1.1 (a progeny BA.2.75), during summer 2022 India. vitro experiments revealed most profoundly resistant variant to BA.2/5 breakthrough infection sera ever more fusogenic than BA.2.75. Notably, breakpoint located in receptor-binding domain spike, each region...
Summary Circulation of SARS-CoV-2 Omicron XBB has resulted in the emergence XBB.1.5, a new Variant Interest. Our phylogenetic analysis suggests that XBB.1.5 evolved from XBB.1 by acquiring F486P spike (S) mutation, subsequent to acquisition nonsense mutation ORF8. Neutralization assays showed similar abilities immune escape between and XBB.1. We determined structural basis for interaction human ACE2 S protein showing overall structures proteins XBB.1.5. The intrinsic pathogenicity hamsters...
Three strains of strictly aerobic, Gram-negative, naphthalene-degrading bacteria isolated from polychlorinated-dioxin-contaminated soil and sediment were characterized. These isolates grew well with naphthalene as the sole carbon energy source, degrading it completely within 24 h incubation. The also degraded dibenzofuran co-metabolically in presence concomitant production yellow intermediate metabolite(s). A 16S rRNA gene sequence analysis revealed that affiliated to genus Novosphingobium...
Induced pluripotent stem cells (iPSCs) are potentially valuable cell sources for disease models and future therapeutic applications; however, inefficient generation the presence of integrated transgenes remain as problems limiting their current use. Here, we developed a new Sendai virus vector, TS12KOS, which has improved efficiency, does not integrate into cellular DNA, can be easily eliminated. TS12KOS carries KLF4, OCT3/4, SOX2 in single vector generate iPSCs from human blood cells. Using...
Melatonin regulates diurnal changes in locomotor activity vertebrates, but the molecular mechanism for this neurohormonal regulation of behavior is poorly understood. Here we show that 7α-hydroxypregnenolone, a previously undescribed avian neurosteroid, mediates melatonin action on rhythms quail. In study, first identified 7α-hydroxypregnenolone and its stereoisomer 7β-hydroxypregnenolone quail brain. These neurosteroids have not been described We then demonstrated acutely increased...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), the causative agent of disease 2019 (COVID-19), is still circulating among humans, leading to continuous evolution. SARS-CoV-2 Omicron JN.1 evolved from a distinct lineage, BA.2.86, spread rapidly worldwide. It unclear why BA.2.86 did not become dominant and was quickly replaced by JN.1, which possesses one amino acid substitution in spike protein (S:L455S) two non-spike proteins NSP6 ORF7b (NSP6:R252K ORF7b:F19L) compared...
Chronic infection with hepatitis B virus (HBV) (chronic HBV infection) places patients at increased risk for liver cirrhosis and hepatocellular carcinoma. Although nucleos(t)ide analogues are mainly used the treatment of HBV, they require long-term administration may lead to emergence drug-resistant mutants. Therefore, identify targets development novel anti-HBV drugs, we screened HBV-suppressive host factors using a plasmid expression library RNA-binding proteins (RBPs). We tested effect...
Objective Although HCV is a major cause of chronic liver disease worldwide, there currently no prophylactic vaccine for this virus. Thus, the development an that can induce both humoural and cellular immunity urgently needed. To create effective vaccine, we evaluated neutralising antibody induction immune responses following immunisation non-human primate model with cell culture-generated (HCVcc). Design accomplish this, 10 common marmosets were immunised purified, inactivated HCVcc in...
Abstract The emergence of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) Omicron variants has led to concerns that ancestral SARS-CoV-2-based vaccines may not be effective against newly emerging subvariants. concept “imprinted immunity” suggests individuals vaccinated with virus-based develop immunity subvariants, such as BQ.1.1 and XBB.1. In this study, we investigated possibility using hamsters. Although natural infection induced antiviral immunity, breakthrough infections in...
Monitoring
BackgroundAlthough several SARS-CoV-2-related coronaviruses (SC2r-CoVs) were discovered in bats and pangolins, the differences virological characteristics between SARS-CoV-2 SC2r-CoVs remain poorly understood. Recently, BANAL-20-236 (B236) was isolated from a rectal swab of Malayan horseshoe bat found to lack furin cleavage site (FCS) spike (S) protein. The comparison its with FCS-deleted (SC2ΔFCS) has not been conducted yet.MethodsWe prepared human induced pluripotent stem cell...
The taxonomic position of two actinomycete strains isolated from soil was studied. isolates contained glutamic acid, alanine and meso-diaminopimelic acid as cell-wall amino acids menaquinone MK-9(H4) madurose in the whole-cell hydrolysate. Phylogenetic analysis revealed that belonged to family Streptosporangiaceae, but not any known genus, formed a monophyletic cluster with Streptosporangium corrugatum. On basis morphological characteristics, phylogenetic DNA-DNA hybridization, name...
Summary Many enveloped viruses induce multinucleated cells (syncytia), reflective of membrane fusion events caused by the same machinery that underlies viral entry. These syncytia are thought to facilitate replication and evasion host immune response. Here, we report co-culture human expressing receptor ACE2 with SARS-CoV-2 spike, results in synapse-like intercellular contacts initiate cell-cell fusion, producing resembling those identify lungs COVID-19 patients. To assess mechanism...