A5101748939- RNA modifications and cancer
- RNA and protein synthesis mechanisms
- RNA Research and Splicing
- Advanced Proteomics Techniques and Applications
- Glycosylation and Glycoproteins Research
- Peptidase Inhibition and Analysis
- Signaling Pathways in Disease
- Monoclonal and Polyclonal Antibodies Research
- Mass Spectrometry Techniques and Applications
- Neurogenetic and Muscular Disorders Research
- Cancer-related gene regulation
- Porphyrin and Phthalocyanine Chemistry
- Cancer-related molecular mechanisms research
- Magnetism in coordination complexes
- Extracellular vesicles in disease
- Heat shock proteins research
- Genetic Neurodegenerative Diseases
- Amyotrophic Lateral Sclerosis Research
- DNA Repair Mechanisms
- Endoplasmic Reticulum Stress and Disease
- RNA Interference and Gene Delivery
- Organic and Molecular Conductors Research
- Protein Degradation and Inhibitors
- Enzyme Structure and Function
- Ubiquitin and proteasome pathways
Tokyo University of Agriculture and Technology
2011-2022
Kyoto University of Education
2021
Kyoto University
2021
Tokyo University of Agriculture
2021
Tokushima University
2021
Keio University
2019
Japan Science and Technology Agency
2007-2017
Okayama University
1991-2015
Iwate University
2012
Tokyo Metropolitan University
1979-2010
During ribosome biogenesis, ribosomal RNAs acquire various chemical modifications that ensure the fidelity of translation, and dysregulation modification processes can cause proteome changes as observed in cancer inherited human disorders. Here, we report complete all 80S determined with quantitative mass spectrometry. We assigned 228 sites 14 different post-transcriptional modifications, most which are located functional regions ribosome. All detected typical eukaryotic RNAs, no...
Protein constituents of the postsynaptic density (PSD) fraction were analysed using an integrated liquid chromatography (LC)-based protein identification system, which was constructed by coupling microscale two-dimensional (2DLC) with electrospray ionization (ESI) tandem mass spectrometry (MS/MS) and automated data analysis system. The PSD prepared from rat forebrain solubilized in 6 m guanidium hydrochloride, proteins digested trypsin after S-carbamoylmethylation under reducing conditions....
One of the strategies functional proteomics, research aiming to discover gene function at protein level, is comprehensive analysis protein−protein interactions related linkage among proteins and cellular machinery better understand basis cell functions. Here, we describe direct nanoflow LC (DNLC) system, which equipped with a fritless high-resolution electrospray interface column packed 1-μm reversed-phase (RP) beads novel splitless gradient elution system operate column. Using RP-DNLC an...
The ubiquitin E3 protein ligase Nedd4-2 is a physiological regulator of the epithelial sodium channel ENaC, which essential for transepithelial Na+ transport and linked to Liddle's syndrome, an autosomal dominant disorder human salt-sensitive hypertension. function negatively regulated by phosphorylation via serum- glucocorticoid-inducible kinase (Sgk1), serves as mechanism inhibit ubiquitination-dependent degradation ENaC. We report here that 14-3-3 proteins participate in this regulatory...
Cyclophilins are a class of ubiquitous proteins with yet unknown function. They were originally discovered as the major binding for immunosuppressant cyclosporin A. The only known catalytic function these in vitro is cis/trans isomerization Xaa-Pro bonds oligopeptides. This became clear after discovery that bovine cyclophilin identical porcine prolyl isomerase. enzyme accelerates slow, proline-limited steps refolding several proteins. Here we demonstrate cyclophilins from man, pig,...
We present the complete chemical structures of rRNAs from eukaryotic model organism, Saccharomyces cerevisiae. The final structures, as determined with mass spectrometry-based methodology that includes a stable isotope-labelled, non-modified reference RNA, contain 112 sites 12 different post-transcriptional modifications, including previously unidentified pseudouridine at position 2345 in 25S rRNA. Quantitative stoichiometric analysis modifications each site indicated 94 were almost fully...
Extracellular vesicle (EV) microRNAs are of major interest as potential diagnostic biomarkers in all cancer types. This study aims to identify miRNA profiles shed microvesicles (sMVs) and exosomes (Exos) secreted from the isogenic colorectal (CRC) cell lines SW480 SW620 evaluate their ability predict CRC. Deep sequencing miRNAs parental lysates (CLs) highly-purified sMVs Exos was performed. We focused on enriched EVs dysregulated metastatic cells (SW620) relative primary (SW480)....
ADVERTISEMENT RETURN TO ISSUEPREVArticleNEXTTwo distinct forms of peptidylprolyl-cis-trans-isomerase are expressed separately in periplasmic and cytoplasmic compartments Escherichia coli cellsToshiya Hayano, Nobuhiro Takahashi, Setsuko Kato, Noboru Maki, Masanori SuzukiCite this: Biochemistry 1991, 30, 12, 3041–3048Publication Date (Print):March 26, 1991Publication History Published online1 May 2002Published inissue 26 March...
Nop56p is a component of the box C/D small nucleolar ribonucleoprotein complexes that direct 2'-O-methylation pre-rRNA during its maturation. Genetic analyses in yeast have shown plays important roles early steps processing. However, precise function remains elusive, especially higher eukaryotes. Here we describe proteomic characterization human (hNop56p)-associated pre-ribosomal complexes. Mass spectrometric analysis purified identified 61 ribosomal proteins, 16 trans-acting factors...
Although interactions between cell surface proteins and extracellular ligands are key to initiating embryonic stem differentiation specific lineages, the plasma membrane protein components of these cells largely unknown. We describe here a group expressed on undifferentiated mouse line D3. These were identified using combination labeling with biotin, subcellular fractionation membranes, mass spectrometry-based identification technology. From 965 unique peptides carrying biotin labels, we...
The recently discovered macrolide FK506 has been demonstrated to have potent immunosuppressive activity at concentrations 100-fold lower than cyclosporin A, a cyclic undecapeptide that is used prevent rejection after transplantation of bone marrow and organs, such as kidney, heart, liver. After the recent discovery A-binding protein cyclophilin identical peptidylprolyl cis-trans isomerase, cellular binding for was found be distinct from but same enzymatic activity. In this study, we isolated...
Abstract Previously we reported that LIM1863 colorectal cancer (CRC) cells secrete three distinct extracellular vesicle subtypes – two subpopulations of exosomes (apical EpCAM-Exos and basolateral A33-Exos) shed microvesicles (sMVs) with protein miRNA signatures. Here, extend our omics approach to understand the fundamental role LIM1863-derived EVs by performing a comprehensive analysis their mRNAs long non-coding RNAs (lncRNAs) using RNA-Seq. We show 2,389 mRNAs, 317 pseudogene transcripts,...
We present a liquid chromatography–mass spectrometry (LC-MS)-based method for comprehensive quantitative identification of post-transcriptional modifications (PTMs) RNA. incorporated an in vitro-transcribed, heavy isotope-labeled reference RNA into sample solution, digested the mixture with number RNases and detected post-transcriptionally modified oligonucleotides quantitatively based on shifts retention time MS signal subsequent LC-MS. This allowed determination quantitation all PTMs...
Protein glycosylation is a common post-translational modification that plays important roles in terms of protein function. However, analyzing the relationship between and function remains technically challenging. This problem arises from fact attached glycans possess diverse heterogeneous structures. We believe first step to elucidate glycan systematically determine status under physiological conditions. Such studies involve differences structure on cell type (tissue), sex, age, as well...
In cytoplasm, the survival of motor neuron (SMN) complex delivers pre-small nuclear RNAs (pre-snRNAs) to heptameric Sm ring for assembly on pre-snRNAs at conserved site [A(U) 4–6 G]. Gemin5, a WD40 protein component SMN complex, is responsible recognizing pre-snRNAs. addition, Gemin5 has been reported specifically bind m 7 G cap. this study, we show that domain both necessary and sufficient binding by isothermal titration calorimetry (ITC) mutagenesis assays. We further determined crystal...
A proteome of a model organism, Caenorhabditis elegans, was analyzed by an integrated liquid chromatography (LC)-based protein identification system, which constructed microscale two-dimensional (2DLC) coupled with electrospray ionization (ESI) tandem mass spectrometry (MS/MS) on high-resolution hybrid spectrometer automated data analysis system. Soluble and insoluble fractions were prepared from mixed growth phase culture the worm C. digested trypsin, fractionated separately 2DLC The...
The 14-3-3 proteins are a family of acidic found mainly in the brain and suggested to have role monoamine synthesis based on their ability activate tyrosine tryptophan hydroxylases presence type II Ca2+/calmodulin-dependent protein kinase. Recently, however, it has been demonstrated that member family, termed Exo1, stimulates Ca(2+)-dependent exocytosis permeabilized adrenal chromaffin cells, suggesting this may influence kinase C-mediated control exocytosis. Here we show C at about 2-fold...