A5103171592- Acute Myeloid Leukemia Research
- Chronic Myeloid Leukemia Treatments
- Hematopoietic Stem Cell Transplantation
- Chronic Lymphocytic Leukemia Research
- Mesenchymal stem cell research
- Neuroblastoma Research and Treatments
- Bone and Joint Diseases
- Acute Lymphoblastic Leukemia research
- Bone health and treatments
- Neutropenia and Cancer Infections
- Eosinophilic Disorders and Syndromes
- Lymphoma Diagnosis and Treatment
- Peptidase Inhibition and Analysis
- PI3K/AKT/mTOR signaling in cancer
- Hematological disorders and diagnostics
- Wnt/β-catenin signaling in development and cancer
- Blood disorders and treatments
- Multiple Myeloma Research and Treatments
- Bone Metabolism and Diseases
- NF-κB Signaling Pathways
- Fibroblast Growth Factor Research
- RNA Interference and Gene Delivery
- Immunotherapy and Immune Responses
- Immune Response and Inflammation
- Telomeres, Telomerase, and Senescence
Kyushu University
2019-2023
Duke University
2016-2021
Kyoto University Hospital
2008-2018
Kyoto University
2009-2015
Abstract IL-7 is a cytokine crucial for development and maintenance of lymphocytes other hematopoietic cells. However, how IL-7–expressing cells are distributed in lymphoid organs not well known. To address this question, we established analyzed IL-7–GFP knock-in mice. Thymic epithelial (TECs) expressed high GFP levels the cortex medulla, as detected with an anti-GFP Ab. mesenchymal also GFP. Flow cytometry analysis suggested that cortical TECs higher than did medullary TECs. In bone marrow,...
Granulopoiesis is tightly regulated to meet host demands during both "steady-state" and "emergency" situations, such as infections. The transcription factor CCAAT/enhancer binding protein β (C/EBPβ) plays critical roles in emergency granulopoiesis, but the precise developmental stages which C/EBPβ required are unknown. In this study, a novel flow cytometric method was developed that successfully dissected mouse bone marrow cells undergoing granulopoiesis into five distinct subpopulations...
Galectins comprise a family of animal lectins that differ in their affinity for β-galactosides. Galectin-9 (Gal-9) is tandem-repeat-type galectin was recently shown to function as ligand T-cell immunoglobin domain and mucin domain-3 (Tim-3) expressed on terminally differentiated CD4(+) Th1 cells. Gal-9 modulates immune reactions, including the induction apoptosis In this study, we investigated effects murine models acute GVH disease (aGVHD). First, demonstrated recombinant human inhibit MLR...
Multiple myeloma (MM) is a malignant neoplasm of plasma cells. Although new molecular targeting agents against MM have been developed based on the better understanding underlying pathogenesis, still remains an incurable disease. We previously demonstrated that β-catenin, downstream effector in Wnt pathway, potential target using RNA interference vivo experimental mouse model. In this study, we screened library more than 100 000 small-molecule chemical compounds for novel Wnt/β-catenin...
Parathyroid hormone (PTH) stimulates hematopoiesis in mouse models. The involvement of osteoblasts this process has been well investigated; however, the effects PTH on human and bone marrow mesenchymal stromal cells (BM-MSCs) are unclear. Here, we show that BM-MSCs contribute to hematopoiesis-stimulating via upregulation cadherin-11 (CDH11). When culture-expanded were stimulated with PTH, their ability expand cocultured CD34(+) hematopoietic progenitor (HPCs) was enhanced. Furthermore, when...
Treatment with Abl tyrosine kinase inhibitors (TKI) drastically improves the prognosis of chronic myelogenous leukemia (CML) patients. However, quiescent CML cells are insensitive to TKI and can lead relapse disease. Thus, research is needed elucidate properties these cells, including their microenvironment, in order effectively target them. Hypoxia known be a common feature solid tumors that contributes therapeutic resistance. Leukemic also able survive proliferate severely hypoxic...
Abstract Growth differentiation factor 15 ( GDF 15) is a pleiotropic cytokine that belongs to the transforming growth factor‐ β superfamily. Elevated serum concentrations of this have been reported in patients with various malignancies. To assess potential roles hematologic malignancies, we measured its levels these diseases. We found were elevated almost all patients, particularly primary myelofibrosis PMF ). Immunohistochemical staining bone marrow BM ) specimens revealed was strongly...
Abstract The transcription factor CCAAT/enhancer-binding protein β (C/EBPβ) regulates the differentiation of a variety cell types. Here, role C/EBPβ expressed by bone marrow mesenchymal stromal cells (BMMSCs) in B-cell lymphopoiesis was examined. size precursor population reduced C/EBPβ-knockout (KO) mice. When from C/EBPβ-KO mice were transplanted into lethally irradiated wild-type (WT) mice, which provide normal microenvironment, restored to level equivalent that generated WT cells. In...
p32/C1qbp regulates mitochondrial protein synthesis and is essential for oxidative phosphorylation in mitochondria. Although dysfunction of impairs fetal development immune responses, its role hematopoietic differentiation remains unclear. Here, we found that affected terminal newly identified erythroid/B-lymphoid progenitors among CD45- Ter119- CD31- triple-negative cells (TNCs) bone marrow. Hematopoietic cell-specific genetic deletion (p32cKO) mice caused anemia B-lymphopenia without...