A5071254876- Virus-based gene therapy research
- Spine and Intervertebral Disc Pathology
- Virology and Viral Diseases
- Cancer Research and Treatments
- Respiratory viral infections research
- CAR-T cell therapy research
- Viral gastroenteritis research and epidemiology
- Animal Virus Infections Studies
- Spinal Fractures and Fixation Techniques
- Viral Infectious Diseases and Gene Expression in Insects
- Cervical and Thoracic Myelopathy
- Immunotherapy and Immune Responses
- Monoclonal and Polyclonal Antibodies Research
- RNA Interference and Gene Delivery
- Spinal Hematomas and Complications
- Bone Tumor Diagnosis and Treatments
- Reproductive System and Pregnancy
- interferon and immune responses
- Infectious Diseases and Tuberculosis
- Liver physiology and pathology
- Medical Imaging and Analysis
- Cardiovascular Function and Risk Factors
- Cardiac Imaging and Diagnostics
- Animal Genetics and Reproduction
- Pancreatic function and diabetes
National Cerebral and Cardiovascular Center
1984-2024
Tottori University
2002-2023
Kawasaki Medical School
2011-2021
Toyama Industrial Technology Center
2002-2019
Tottori University Hospital
2014-2019
Gynecologic Oncology Group
2017
RIKEN Center for Brain Science
2017
Keio University
2017
Fukuoka Tokushukai Hospital
2015
Kumamoto University
1997-2014
Oncolytic vaccinia virus encoding IL-7 and IL-12 facilitates immune checkpoint blockade in treatment-resistant solid tumors.
Abstract Live attenuated Edmonston B strain of measles virus (MV-Edm) is a potent and specific oncolytic agent, but the mechanism underlying its tumor selectivity unknown. The causes cytopathic effects (CPEs) extensive syncytial formation in cells minimal damage or cell killing normal cells. CPE dependent on expression viral proteins presence CD46, major cellular receptor MV-Edm. Using virally encoded soluble marker peptide to provide quantitative readout level gene expression, we determined...
CD4+CD25high regulatory T cells (Tregs) play a critical role in self-tolerance, as seen murine autoimmunity. Studies on Tregs human autoimmunity have focused primarily peripheral blood samples. A study targeting diseased tissue should identify direct relationships between and Peripheral samples were collected from 91 patients with primary biliary cirrhosis (PBC), 28 immediate relatives, 41 healthy controls, Treg frequencies determined percentage of CD4+TCR-αβ+ cells. tissue-targeted...
Abstract Although oncolytic virotherapy is a promising anticancer therapy, antitumor efficacy hampered by low tumor selectivity. To identify potent and selective virotherapy, we carried out large-scale two-step screening of 28 enteroviral strains found that coxsackievirus B3 (CVB3) possessed specific activity against nine human non–small cell lung cancer (NSCLC) lines. CVB3-mediated cytotoxicity was positively correlated with the expression viral receptors, adenovirus receptor,...
Foxp3 + CD4 CD25 regulatory T (Treg) cells and immunoregulatory enzyme indoleamine 2,3‐dioxygenase (IDO) play an important role in immunoregulation. Accumulating evidence shows that IDO Treg have potent properties for immune escape cancer. To evaluate the expression of localization development progression uterine cervical cancer, primary metastatic lesions were studied using immunohistochemistry. tumor appeared intraepithelial neoplasia (CIN)‐3 cervix marked microinvasive cancer was...
To clarify the effect of mechanical stress on hypertrophy ligamentum flavum (LF), we compared thicknesses, histological findings, and total collagen content 161 lumbar LF in two groups defined by radiographic signs. with one or more signs spondylolisthesis, disk degeneration, vacuum phenomenon, traction spur, facet joint osteoarthrosis were considered degenerative; all others nondegenerative. Thickness was measured computed tomographic scan, Woessner's method. We found that ligament thicker...
We have isolated a cDNA (cNPK1) that encodes predicted protein kinase of 690 amino acids from suspension cultures tobacco cells. The deduced sequence is closely related to those the kinases encoded by STE11 and BCK1 genes Saccharomyces cerevisiae byr2 gene Schizosaccharomyces pombe. Byr2 function in yeast mating pheromone response pathways, acts downstream C homolog PKC1 gene, which essential for normal growth division Overexpression cells truncated form cNPK1, only putative catalytic...
We have shown by site-directed mutagenesis that the sequence between positions -69 and -40 of mouse alpha A-crystallin gene is crucial for tissue-specific expression in a transfected lens epithelial cell line transformed with early region simian virus 40. Gel retardation experiments synthetic oligodeoxynucleotides revealed nuclear protein which bound specifically to palindromic 5'-GGGAAATCCC-3' at -66 -57 promoter. By screening bacteriophage lambda gt11 library cells, we isolated...
The initial recognition and binding of adenovirus vector to the host cell surface is mediated by interaction between fiber knob protein its receptor, coxsackievirus receptor (CAR). This natural tropism needs be ablated in order achieve targeted gene transfer. To this end, we noted that serotype 40 (Ad40) contains two distinct long short fibers; unable recognize CAR, while binds CAR. We generated 5-based mutants with chimeric Ad40-derived fibers, which were composed either or shafts together...
To ascertain the pathomechanism of spontaneous regression lumbar disc hemiation, histological and immunohistochemical studies on 100 hemiated discs were performed. Inflammatory findings such as cell infiltration, neovascularization, granulation observed in 16.9% protruded discs, 81.8% subligamentously extruded 100% transligamentously disks, 80% sequestrated discs. The infiltrated cells composed mainly macrophages a small number T lymphocytes. Furthermore, infiltration was more prominent...
Abstract Among the best-characterized genetic alterations in gliomas is amplification of epidermal growth factor receptor (EGFR) gene, present ∼40% glioblastoma multiforme, and frequently associated with EGFRvIII gene rearrangement. We have previously shown that attenuated vaccine strains measles virus potent antitumor activity against gliomas, identified H protein mutations, which ablate recognition natural receptors CD46 SLAM. Retargeted recombinant viruses were generated from...
A new hernia model that simulates human disc herniations was developed in rabbits. The herniated discs were examined by gross appearance and histology production of tumor necrosis factor alpha, interleukin-1beta, monocyte chemoattractant protein-1 investigated.To clarify the early mechanism spontaneous resorption.Macrophage infiltration is essential for resorption. However, surgically removed tissues existing animal models are not suitable analyzing macrophage infiltration. Recently, we have...
To determine the potential role of PTEN in process endometrial carcinogenesis, we examined a series carcinoma and hyperplasia uterine corpus for presence mutation. The entire coding region gene was screened mutations by single-strand conformation polymorphism analysis, were confirmed sequencing. We detected 14 57 carcinomas (13 50 endometrioid adenocarcinomas 1 7 nonendometrioid adenocarcinomas) 73 hyperplasias (1 24 simple without atypia, none 16 complex 6 33 with atypia). Most (88%)...
A retargeted measles virus strain MV-GFP-HAA-scEGFR was generated by engineering the MV-NSe Edmonston vaccine to incorporate both CD46 (Y481A) and signaling lymphocyte activation molecule (SLAM) (R533A) ablating mutations in hemagglutinin protein combination with display of a single-chain antibody against epidermal growth factor receptor (EGFR) at C terminus hemagglutinin. The unmodified MV-GFP used as positive control. Specificity EGFR demonstrated non-permissive Chinese hamster ovary (CHO)...
PROBLEM: The systemic T helper 1/T 2 (Th1/Th2) cytokine balance during normal human pregnancy is controversial, and observations about the in postpartum period have only been reported for up to 3 months. METHOD: Whole‐blood, from 83 healthy pregnant women, 80 31 non‐pregnant women was stimulated with phorbol 12‐myristate 13‐acetate (PMA) ionomycin, levels of cytokines supernatant were measured by enzyme‐linked immunosorbent assay (ELISA). RESULTS: production all decreased pregnancy,...
Secretory leukocyte protease inhibitor (SLPI) is a potent of human elastase. SLPI transcripts in the cervical tissue were detected during menstrual cycle by reverse transcription–polymerase chain reaction (RT–PCR). Western blot analysis revealed that intensity protein ovulatory phase was stronger than other phases. Immunohistochemistry using an anti-SLPI polyclonal antibody positive staining epithelial cells endocervix. also present mucus. Again follicular phase. The concentrations and...