A5069651723- Immunotherapy and Immune Responses
- T-cell and B-cell Immunology
- Immune Cell Function and Interaction
- Renal Transplantation Outcomes and Treatments
- Immune Response and Inflammation
- Organ Transplantation Techniques and Outcomes
- RNA Interference and Gene Delivery
- CAR-T cell therapy research
- Organ and Tissue Transplantation Research
- Signaling Pathways in Disease
- Xenotransplantation and immune response
- Liver physiology and pathology
- Immune cells in cancer
- Mast cells and histamine
- Reproductive System and Pregnancy
- Hematopoietic Stem Cell Transplantation
- Cytomegalovirus and herpesvirus research
- Transplantation: Methods and Outcomes
- Cell Adhesion Molecules Research
- Neuroinflammation and Neurodegeneration Mechanisms
- Virus-based gene therapy research
- PI3K/AKT/mTOR signaling in cancer
- Immunodeficiency and Autoimmune Disorders
- Toxin Mechanisms and Immunotoxins
- Pharmacological Effects and Toxicity Studies
University of Pittsburgh
2016-2025
Riverside Transplantation Institute
2009-2024
University of Strathclyde
2019
Creative Commons
2018
University of Bari Aldo Moro
2016
University of Pittsburgh Medical Center
2000-2014
Children's Hospital of Pittsburgh
2003-2014
Geriatric Research Education and Clinical Center
2014
Huashan Hospital
2014
Fudan University
2014
Abstract The ability of dendritic cells (DC) to regulate Ag-specific immune responses via their influence on T regulatory (Treg) may be key potential as therapeutic tools or targets for the promotion/restoration tolerance. In this report, we describe maturation-resistant, rapamycin (RAPA)-conditioned DC, which are markedly impaired in Foxp3− cell allostimulatory capacity, favor stimulation murine alloantigen-specific CD4+CD25+Foxp3+ Treg. This was distinct from control especially following...
We have shown previously that granulocyte-macrophage colony-stimulating factor-stimulated mouse bone marrow-derived MHC class II+dendritic cell (DC) progenitors are deficient in surface expression of the costimulatory molecules B7-1(CD80) and B7-2 (CD86) can induce alloantigen-specific T-cell anergy vitro. To test vivo relevance these findings, 2×106B10 (H2b) DC (NLDC 145+, II+, B7-1dim, B7-2-/dim) induced hyporesponsiveness vitro were injected systemically into normal C3H (H2k) recipients....
Recent evidence suggests that functional deficiency in regulatory T cells (Tregs), an innate immunomodulator, exacerbates brain damage after cerebral ischemia. We therefore evaluated the effect of Treg transfer rodent models ischemic stroke and further investigated mechanism underlying Treg-afforded neuroprotection.We examined therapeutic potential Tregs mechanisms neuroprotection vivo 2 vitro Treg-neutrophil cocultures using a combined approach including cell-specific depletion, gene...
IL-33 administration is associated with facilitation of Th2 responses and cardioprotective properties in rodent models. However, heart transplantation, the mechanism by which IL-33, signaling through ST2L (the membrane-bound form ST2), promotes transplant survival unclear. We report that administration, while facilitating responses, also increases immunoregulatory myeloid cells CD4(+) Foxp3(+) regulatory T (Tregs) mice. expands functional myeloid-derived suppressor cells, CD11b(+) exhibit...
Abstract MHC-mismatched liver grafts are accepted spontaneously between many mouse strains. The underlying mechanism(s) is unclear. In the B10 (H2(b)) to C3H (H2(k)) strain combination used in this study, donor T cells within were rapidly replaced 2 4 days of transplantation with those recipient. Freshly isolated graft-infiltrating harvested on and 7 exhibited strong CTL responses against alloantigens. activity was reduced substantially, however, by day 14, although levels precursors spleen...
IL-17 is a T cell-derived cytokine that stimulates stromal cells and macrophages to secrete proinflammatory cytokines. We hypothesized might play role in alloimmune responses, interference with its activity suppress allograft rejection. IL-17R:Fc or control IgG was added at the start of mouse MLR administered i.p. (100-500 microg/day) for different durations post-transplant murine recipients MHC-mismatched cardiac allografts. (50-200 microg/ml) markedly inhibited cell proliferation vitro...
The functional maturation of dendritic cells (DC) and other antigen-presenting is believed to reflect the upregulation cell surface major histocompatibility complex (MHC) class II T costimulatory molecules, especially CD28 ligands B7-1 (CD80) B7-2 (CD86). In this study, we propagated exhibiting characteristics DC precursors from bone marrow (BM) B10 mice (H-2b; I-A+) in response granulocyte-macrophage colony stimulating factor (GM-CSF). methods used were similar those employed previously...
Activation of T cells is induced efficiently by dendritic (DC), but little known about the role DC in regulation cell death. In this study, highly purified (DEC-205+, MHC class II(high), B7-1+ [CD80+], B7-2high [CD86high], CD40+, CD11c+) grown from normal mouse bone marrow granulocyte-macrophage CSF + IL-4 were found to express FasL (CD95L) mRNA reverse transcriptase PCR and uniformly both flow cytometric immunocytochemical analyses. These cells, not propagated FasL-deficient (B6.gld) mice,...
Within 1 wk of liquid culture in granulocyte/macrophage colony-stimulating factor (GM-CSF), normal B10 BR (H-2k I-E+) mouse liver nonparenchymal cells (NPC) formed loosely adherent myeloid cell clusters that have been shown to contain dendritic (DC) progenitors similar studies blood or bone marrow. Mononuclear progeny released from these at and beyond 4 d exhibited distinct morphology were actively phagocytic. After 6-10 culture, strongly expressed CD45, CD11b, heat stable antigen, CD44....