A5000802911- Pancreatic function and diabetes
- Clinical Nutrition and Gastroenterology
- Veterinary Pharmacology and Anesthesia
- Opioid Use Disorder Treatment
- Animal Nutrition and Physiology
- Diet and metabolism studies
- Organ Transplantation Techniques and Outcomes
- Rabbits: Nutrition, Reproduction, Health
- Organ and Tissue Transplantation Research
- Metabolism, Diabetes, and Cancer
- Diabetes Treatment and Management
- Pharmacological Effects and Assays
- Transplantation: Methods and Outcomes
- Animal Behavior and Welfare Studies
- Animal Disease Management and Epidemiology
- Antimicrobial Peptides and Activities
- Infectious Aortic and Vascular Conditions
- Pancreatic and Hepatic Oncology Research
- Metabolomics and Mass Spectrometry Studies
- Polymer Surface Interaction Studies
- MRI in cancer diagnosis
- Toxoplasma gondii Research Studies
- Xenotransplantation and immune response
- Animal testing and alternatives
- Aldose Reductase and Taurine
Swedish University of Agricultural Sciences
2014-2025
Columbia University
2023-2025
Background. Intestinal transplantation (ITx) has the highest rate of rejection among solid organ grafts. We aimed to study pathophysiology after ITx but lacked a tool for assessing cellular responses within graft. Therefore, we developed novel mixed lymphocyte reaction (MLR) assay investigate immune in Methods. samples were collected, decontaminated, and processed into single-cell suspensions from 9 swine 2 patients that underwent ITx. Debris was removed using gradient centrifugation. The...
The ability to perform accurate continuous glucose monitoring without blood sampling has revolutionised the management of diabetes. Newer methods that can allow measurements during longer periods are necessary substantially improve patients' quality life. This paper presents an alternative method for which is based on electrical impedance spectroscopy. A battery-less implantable bioimpedance spectroscope was designed, built, and used in vivo study pigs. After a recovery period 14 days post...
Diabetes mellitus is increasing worldwide and reliable animal models are important for progression of the research field. The pig a commonly used large model in diabetes present study aimed to refine oral glucose tolerance test (OGTT) young growing pigs, as well describing intravenous (IVGTT) same age group. refined porcine OGTT will reflect that children adolescents. Eighteen pigs were obtained one week after weaning trained two weeks bottle-feed solution, mimicking human OGTT. subsequently...
Buprenorphine is one of the most used analgesics for postoperative pain in rabbits. The recommended dose rabbits (0.01-0.05 mg/kg) same intravenous (IV), intramuscular (IM), and subcutaneous (SC) administration, despite lack pharmacokinetic data. Five male five female New Zealand White (mean ± SD body weight 3.1 0.3 kg) were administered 0.05 mg/kg buprenorphine by IV, IM SC routes 0.1 route, a cross-over design with two-week wash-out periods between treatments. Blood was collected before,...
The effects of early supplementation with oat β-glucan during the suckling period on piglet gut microbiota composition, concentrations short-chain fatty acids, and physiological markers were assessed. Fifty piglets from five litters, balanced for sex birth weight, divided within litters into two treatment groups: control. Piglets in group received supplement three times/week day 7 age until weaning. Rectal swab samples collected 10 per (balanced across litters) week 1 to 4, plasma at 1, 3, 4...
The effects of streptozotocin (STZ) were studied in eight high-health herd-certified Yorkshire × Swedish Landrace pigs (32.5 ± 2.6 kg initial body weight [BW]), and an insulin treatment protocol was developed to re-establish their metabolisms. A single intravenous dose 150 mg STZ/kg BW successfully induced hyperglycaemia alterations fat protein Within 13 h post-STZ blood glucose concentration had fallen a range 1.3 4.7 mmol/L. Hypoglycaemia promptly treated with 0.5 g glucose/kg...
Pigs are commonly used in diabetes research due to their many physiological similarities humans. They especially useful imaging procedures because of large size. However, achieve the pig must lie completely still, and thus needs be anaesthetized. Most anaesthetic drugs laboratory animals affect carbohydrate metabolism by inhibition insulin release. The aim this pilot study was primarily develop an protocol for pigs that did not have effect on blood glucose levels throughout 3 h anaesthesia;...
Perfusion of the pancreas and islets Langerhans is sensitive to physiological stimuli dysregulated in metabolic disease. Pancreatic perfusion can be assessed by both positron emission tomography (PET) magnetic resonance imaging (MRI), but methods have not been directly compared or benchmarked against gold-standard microsphere technique.Pigs (n = 4) were examined [15O]H2O PET intravoxel incoherent motion (IVIM) MRI technique simultaneously using a hybrid PET/MRI scanner. The pancreatic was...
The pig is commonly used in renal transplantation studies since the porcine kidney resembles human kidney. To meet requirements of intense caretaking and examination without stress, a 2-week socialisation training programme was developed. Conventional cross-breed pigs ( n = 36) with high health status were trained for 15 min/day four-step before transplantation. systematic resulted calm animals, which allowed ultrasound examination, blood sampling urine restraint. When 2-methacryloyloxyethyl...
Animal models of human diseases are important in biomedical research. When using animals for scientific purposes, the 3Rs (replace, reduce, refine) should be considered. Refinement animal is essential to ensure best use animals, which ethical reasons and retrieve reliable research data. The present publication describes improvements an oral glucose tolerance test (OGTT) model pigs published 2016. Historical data from 42 were used describe training technique over six years. Pigs various...
In animal studies on bone healing, the effect of housing space and physical activity are seldom taken into account. Bone formation was evaluated in New Zealand White rabbits (mean ± SEM BW: 3.9 0.11 kg) with a critical defect after 12 weeks rehabilitation pair-housing 3 m2 large floor pens (Floor, n = 10) or standard single 0.43 cages (Cage, 10). randomised full-factorial study, replica calcium phosphate cement (CPC, autologous (AB, implanted unilateral 20 mm radius defect. Post-mortem,...
Introduction Glucagon-like peptide-1 (GLP-1) increases insulin secretion from pancreatic beta-cells and GLP-1 receptor (GLP-1R) agonists are widely used as treatment for type 2 diabetes mellitus. Studying occupancy of the GLP-1R in various tissues is challenging due to lack quantitative, repeatable assessments density. The present study aimed describe quantitative distribution GLP-1Rs by endogenous during oral glucose tolerance test (OGTT) pigs, a species that biomedical research model...
An amendment to this paper has been published and can be accessed via the original article.