A5102816467- Xenotransplantation and immune response
- Renal Transplantation Outcomes and Treatments
- Pluripotent Stem Cells Research
- T-cell and B-cell Immunology
- Tissue Engineering and Regenerative Medicine
- Organ Transplantation Techniques and Outcomes
- Dermatology and Skin Diseases
- Animal Genetics and Reproduction
- Animal testing and alternatives
- Renal Diseases and Glomerulopathies
- Animal Ecology and Behavior Studies
- Complement system in diseases
- Pharmacological Effects of Natural Compounds
- Psoriasis: Treatment and Pathogenesis
- Stress Responses and Cortisol
- Genetically Modified Organisms Research
- Bee Products Chemical Analysis
- Acne and Rosacea Treatments and Effects
- Clinical Nutrition and Gastroenterology
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Immunotoxicology and immune responses
- Colorectal and Anal Carcinomas
- Intellectual Property and Patents
- Rheumatoid Arthritis Research and Therapies
- Autoimmune and Inflammatory Disorders
Wake Forest University
2024-2025
SUNY Downstate Health Sciences University
2023
State University of New York
2023
Global Cancer Institute
2016
Harvard University
2004-2015
Massachusetts General Hospital
2004-2015
Pepperdine University
2014
This laboratory has previously demonstrated the induction of allogeneic tolerance by vascularized thymic lobe (VTL) transplantation in miniature swine. We report here our initial attempt to induce VTL clinically relevant, discordant, pig-to-baboon model xenotransplantation.Six baboons received xenografts hDAF VTLs. Four these also omental tissue implants. All recipients were treated with an immunosuppressive conditioning regimen that included thymectomy, splenectomy, extracorporeal...
Background. We have previously shown that a 12-day treatment with cyclosporine A (CyA) facilitates induction of tolerance to class-I disparate kidneys, as demonstrated by acceptance second, donor-matched kidneys without immunosuppression. In the present study, we examined 1) duration in absence donor antigen and 2) pathway recognition determining maintenance or loss tolerance. Methods. Seventeen miniature swine received mismatched 12 days CyA, second immunosuppression at 0, 1, 3, 4 months...
Survival of ABO-mismatched kidneys with stable renal function despite the persistence anti-ABO antibodies is called accommodation. The mechanism accommodation unclear, but may involve complement regulatory proteins such as CD59. development alpha-1,3-galactosyltransferase knock-out (GalT-KO) swine that produce anti-Gal provides a large animal model capable determining role in accommodation.ELISA and antibody fluorescence-activated cell sorting were used to examine rate expression age. Major...
Background. We have previously reported life-supporting kidney xenograft-survival greater than 80 days using a steroid-free antithymocyte globulin (ATG)-based induction regimen (ATG regimen) in GalT-KO pig-to-baboon thymokidney (TK) model. evaluated two regimens, newly developed anti-monkey CD3 recombinant immunotoxin (anti-CD3 rIT) and an anti-human CD2 antibody (LoCD2), by assessing T-cell depletion (TCD) graft survival. Methods. Four baboons received anti-CD3 rIT; the time course of TCD...
Weiner J, Yamada K, Ishikawa Y, Moran S, Etter Shimizu A, Smith RN, Sachs DH. Prolonged survival of GalT‐KO swine skin on baboons. Xenotransplantation 2010; 17: 147–152. © 2010 John Wiley & Sons A/S. Abstract: Background: Allogeneic is currently the best alternative to autologous as a temporary treatment for severe burns, but it has several drawbacks. As potential alternative, we have evaluated skin, which lacks expression Gal epitope, investigate effect eliminating this epitope...
We have previously demonstrated that long-term tolerance (LTT) of an MHC class-I mismatched renal allograft can be achieved with a short course cyclosporine. In order to examine regulatory mechanisms underlying in this model, we assessed the contributions factors within graft and peripheral blood for their relative roles maintenance stable tolerance. Twelve LTT recipients primary kidneys were subjected treatment consisting donor-specific transfusion followed by leukapheresis, remove...
Clinical intestinal transplantation (Int-Tx) is limited by high rates of rejection, infection, and graft versus host disease. To improve clinical outcomes eliminate the comorbidities associated with chronic immunosuppression, induction donor-specific tolerance to grafts desirable, especially in pediatric population. This study determined ability facilitate major histocompatibility complex (MHC)-inbred miniature swine.Seven MGH-miniature swine received heterotopic grafts, two across...
Long-term tolerance of class I disparate renal allografts in miniature swine can be induced by a short course cyclosporine and persists for 3 to 4 months after grafts are removed. Donor peptide immunization 6 weeks graftectomy tolerated kidneys leads sensitization, but donor skin do not. Here, we tested the hypothesis that prevent rejection simultaneous administration grafting.Miniature underwent bilateral nephrectomy I-mismatched transplantation with 12-day A induce long-term tolerance....
Abstract Since α‐1,3‐galactosyltransferase knockout (GalT‐ KO ) pigs became available, there has been an increasing interest in non‐Gal natural antibody ( nA b)‐mediated xenograft rejection. To better understand mechanisms of b‐mediated rejection, a simple small animal model without gene manipulation would be extremely valuable. Here, we tested whether the Chinese tree shrew CTS ), which is small‐sized mammal that phylogenetically close to primates, could serve as for discordant Methods...
Nishimura, H.1; Shimizu, A.2; Griesemer, A.3; Scalea, J. R.1; Moran, S.4; Weiner, J.5; Gillon, B.1; Fishman, J.3; Robson, S. C.3; Sykes, M.3; Sachs, D. H.5; Yamada, K.5 Author Information
P929 Aims: Previous studies have demonstrated the importance of an intact thymus in induction tolerance to class I-mismatched renal allografts by Cyclosporin miniature swine. A short course FK506 has subsequently been shown induce fully mismatched this large animal model. In present study, we assessed whether thymectomy likewise interferes with tolerance. Methods: Recipients MHC disparate were treated a 12-day (0.15 mg/kg/day continuous i.v. infusion), doses adjusted maintain trough levels...
Liang, F.; Griesemer, A.; Scalea, J. R.; Wamala, I.; Tena, Moran, S.; Yamada, K.; Huang, C. Sachs, D. H. Author Information
Background: We have previously reported that 12 days of high-dose Cyclosporine A (CyA) uniformly facilitates tolerance induction for class-I disparate renal allografts in miniature swine. This persists even when the primary graft is removed and immediately replaced with a donor-matched kidney without immunosuppression. also 3-month period absence donor antigen represents borderline time at which lost. In this study, we examined roles thymus loss tolerance. Methods: Six swine recipients...