A5001524481- Coronary Interventions and Diagnostics
- Atherosclerosis and Cardiovascular Diseases
- Protease and Inhibitor Mechanisms
- Cell Adhesion Molecules Research
- Antiplatelet Therapy and Cardiovascular Diseases
- Lipoproteins and Cardiovascular Health
- Viral gastroenteritis research and epidemiology
- Angiogenesis and VEGF in Cancer
- Virus-based gene therapy research
- Immune Cell Function and Interaction
- Tissue Engineering and Regenerative Medicine
- Electrospun Nanofibers in Biomedical Applications
- Cerebrovascular and Carotid Artery Diseases
- Mesenchymal stem cell research
- Cardiovascular Disease and Adiposity
- Respiratory viral infections research
- Protein Kinase Regulation and GTPase Signaling
- Acute Myocardial Infarction Research
- Adipokines, Inflammation, and Metabolic Diseases
- Pharmacological Effects and Assays
- Infant Nutrition and Health
- Mosquito-borne diseases and control
- Inflammatory Biomarkers in Disease Prognosis
- Virology and Viral Diseases
- Cardiac Imaging and Diagnostics
University of Pennsylvania
2007-2016
Hospital of the University of Pennsylvania
2007-2015
Washington Hospital
2013
MedStar Washington Hospital Center
2013
Hospital for Sick Children
2011
Boston Scientific (United States)
2011
Thomas Jefferson University
2008
GlaxoSmithKline (United States)
2008
Gates (United States)
2007
Medical University of South Carolina
1995-2002
Although mesenchymal stem cells (MSCs) show promising signs in reducing myocardial infarct (MI) size, the safety of endomyocardial delivery and most efficacious dose is unknown.Three days after MI, female Yorkshire swine (25-32 kg, age 2 months, n = 32) were randomized to one three MSC doses (2.4 x 10(7), 2.4 10(8), 4.4 10(8) cells) or vehicle control. Animals sacrificed at 12 weeks. There no issues related cell all animals tolerated procedure. By magnetic resonance imaging size (g) was...
Objectives— The relationship between specific gene regulation and subsequent development progression of atherosclerosis is incompletely understood. We hypothesized that genes in the vasculature related to cholesterol metabolism, inflammation, insulin signaling pathways are differentially regulated a site-specific time-dependent manner. Methods Results— Expression 59 obtained from coronary, carotid, thoracic aortic arteries were characterized diabetic (DM)/hypercholesterolemic (HC) swine...
Atherosclerosis is an inflammatory process leading to enhanced cellular proliferation, apoptosis, and vasa vasorum (VV) neovascularization. While both diabetes mellitus (DM) hypercholesterolemia (HC) predispose atherosclerosis, the precise interaction of these risk factors unclear. Akt a central node in signaling pathways important for inflammation, we hypothesized that DM/HC would lead aberrant advanced, complex atherosclerosis. DM was induced pigs by streptozotocin HC high-fat diet....
Objective— To prospectively evaluate whether the development of fibroatheromas exhibiting features potential instability can be detected and predicted by serial invasive imaging. Methods Results— Multivessel intravascular ultrasound near infrared spectroscopy (NIRS) were performed in diabetic/hypercholesterolemic pigs 3, 6, 9 months after induction. Animals euthanized at histological/immunohistochemical evaluation arteries was (n=304 arterial segments). Intravascular demonstrated, over time,...
Abstract Bone marrow derived endothelial progenitor cells (EPCs) are early precursors of mature which replenish aging and damaged cells. The authors studied a diabetic swine model to determine if induction DM adversely affects either bone or circulating EPCs whether HMG‐CoA reductase inhibitor (statin) improves development recruitment in the absence cholesterol lowering. Streptozotocin was administered Yorkshire pigs induce DM. One month after induction, were treated with atorvastatin...
Activation of alpha 2-adrenergic receptors (alpha 2-AR) in pancreatic beta-cells inhibits insulin secretion response to various stimuli, and acute or long-term regulation 2-AR receptor-mediated effects may influence the tissue glucose dishomeostasis. As an initial approach this issue, we determined effect metabolic hormonal treatments on expression coupling beta-cell lines HIT-T15 RIN-5AH. Radioligand binding studies ([3H]RX-821002) RNA blot analysis indicate that both express 2A/D-AR...
CMV infections have been linked to vasculopathies like atherosclerosis and Scleroderma. infects vascular endothelium with intermittent shedding of the virus development latency.We adopted a model arteritis, developed by Presti et al. (1998), triggered murine cytomegalovirus (MCMV) infection. Our studies focused on neointima formation. Groups mice include: 1) immunocompetent 129S, 2) 129S receiving whole body irradiation MCMV, 3) IFN-gammaR-/- 4) MCMV irradiation.Mice were inoculated (5 x...
Despite systemic exposure to risk factors, the circulatory system develops varying patterns of atherosclerosis for unclear reasons. In a porcine model, we investigated relationship between site-specific lesion development and inflammatory pathways involved in coronary arteries (CORs) distal abdominal aortas (AAs).Diabetes mellitus (DM) hypercholesterolemia (HC) were induced 37 pigs with 3 healthy controls. Site-specific plaque was studied by comparing severity, macrophage infiltration, gene...
Background— Animal models used to gain insight into the vascular response drug-eluting stents are generally juvenile and nonatherosclerotic, whereas placed in patients with complex atherosclerosis comorbidities. Hence, reflecting these complexities needed help elucidate effects of stents. We compared responses bare metal stent (BMS) paclitaxel-eluting (PES) implantation a diabetic/hypercholesterolemic (DM/HC) porcine model advanced coronary standard model. Methods Results— Two studies using...
Evaluate the effects of pressure and duration intracoronary (IC) infusion mesenchymal stem cells (MSCs) on delivery efficiency safety after myocardial infarction (MI).Standard IC MSCs can lead to intravascular plugging reduced coronary blood flow. The optimal is unknown.Immediately MI pigs were randomized 1 3 protocols 5 x 10(7) iron-fluorescent microspheres labeled MSCs, control received 2 ml infusions at ml/min (five times), very high flow rate (VHFR) a single 10 60 (HFR) group 20 ml/min....
We aimed to determine whether intravascular ultrasound (IVUS) and near infrared spectroscopy (NIRS) could identify arteries which would subsequently develop a thin-cap fibroatheroma (TCFA).Three-vessel angiography, IVUS NIRS evaluations were performed at three, six nine months after induction of diabetes mellitus hypercholesterolaemia in 13 Yorkshire pigs (n=37 arteries). In vivo total arterial plaque plus media (P+M) area, echo-attenuated (AP) area by IVUS, lipid core burden index (LCBI)...
Resident cells of the respiratory and gastrointestinal tracts, including epithelial fibroblast cells, are initial sites entry for many viral pathogens. We investigated role that these play in inflammatory process response to infection with reovirus 1/L. In A549 human bronchial or HT-29 colonic interferon (IFN)-β, regulated on activation T cell expressed secreted (RANTES), IFN-γ-inducible protein (IP)-10, interleukin-8 were upregulated regardless whether infected replication-competent...
Introduction: Thin-cap fibroatheromas are the pathologic cause of acute coronary syndromes and ischemic sudden death. Near infrared spectroscopy (NIRS) detects lipid concentration in arterial wall intravascular ultrasound (IVUS) identifies necrotic cores vascular remodeling. We hypothesized that concurrent use these two invasive technologies could predict development progression high-risk as determined by histology. Methods: Multi-vessel angiography, IVUS NIRS were performed 3, 6 9 months...