A5088174034- Legume Nitrogen Fixing Symbiosis
- Plant nutrient uptake and metabolism
- Aquaculture disease management and microbiota
- Protist diversity and phylogeny
- MicroRNA in disease regulation
- RNA Interference and Gene Delivery
- Bacteriophages and microbial interactions
- Pediatric Hepatobiliary Diseases and Treatments
- Bacterial Genetics and Biotechnology
- Genomics and Phylogenetic Studies
- Extracellular vesicles in disease
- RNA Research and Splicing
- Microbial Community Ecology and Physiology
- RNA and protein synthesis mechanisms
- Vibrio bacteria research studies
- Fish Ecology and Management Studies
Justus-Liebig-Universität Gießen
2014-2016
Extracellular vesicles (EVs) mediate targeted cellular interactions in normal and pathophysiological conditions are increasingly recognised as potential biomarkers, therapeutic agents drug delivery vehicles. Based on their size biogenesis, EVs classified exosomes, microvesicles apoptotic bodies. Due to overlapping ranges the lack of specific markers, these classes cannot yet be distinguished experimentally. Currently, it is a major challenge field define robust sensitive technological...
Imaging flow cytometry (IFC) is a powerful tool which combines with digital microscopy to generate quantitative high-throughput imaging data. Despite various advantages of IFC over standard cytometry, widespread adoption this technology for studies in aquatic sciences limited, probably due the relatively high equipment cost, complexity image analysis-based data interpretation and lack core facilities trained personnel. Here, we describe application examine phagocytosis particles including...
Quorum sensing of Sinorhizobium meliloti relies on N-acyl-homoserine lactones (AHLs) as autoinducers. AHL production increases at high population density, and this depends the synthase SinI two transcriptional regulators, SinR ExpR. Our study demonstrates that ectopic expression gene rne, coding for RNase E, an endoribonuclease is probably essential growth, prevents accumulation AHLs detectable levels. The rne led to a higher level mRNA lower sinI independently presence ExpR, receptor, AHLs....
Small open reading frames (sORFs) and genes for non-coding RNAs are poorly investigated components of most genomes. Our analysis 1391 ORFs recently annotated in the soybean symbiont Bradyrhizobium japonicum USDA 110 revealed that 78% them contain less than 80 codons. Twenty-one these sORFs conserved or outside Alphaproteobacteria similar to found transposable elements, line with their broad distribution. Stabilizing selection was demonstrated proteomic evidence bll1319_ISGA which is at...
The ribonucleases (RNases) E and J play major roles in E. coli Bacillus subtilis, respectively, co-exist Sinorhizobium meliloti. We analysed S. meliloti 2011 mutants with mini-Tn5 insertions the corresponding genes rne rnj found many overlapping effects. observed similar changes mRNA levels, including lower levels of motility chemotaxis related flaA, flgB cheR higher ndvA (important for glucan export). acyl-homoserine lactone (AHL) were also during exponential growth both RNase mutants,...
Up to now, very small protein-coding genes have remained unrecognized in sequenced genomes. We identified an mRNA of 165 nucleotides (nt), which is conserved Bradyrhizobiaceae and encodes a polypeptide with 14 amino acid residues (aa). The harboring unique Shine-Dalgarno sequence (SD) length 17 nt was localized predominantly the ribosome-containing P100 fraction Bradyrhizobium japonicum USDA 110. Strong interaction between 30S ribosomal subunits demonstrated by their co-sedimentation sucrose...