A5009242761- Influenza Virus Research Studies
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Antibiotic Use and Resistance
- Clostridium difficile and Clostridium perfringens research
- Bacterial Identification and Susceptibility Testing
- SARS-CoV-2 detection and testing
- Nosocomial Infections in ICU
- Vaccine Coverage and Hesitancy
- COVID-19 epidemiological studies
- Health Systems, Economic Evaluations, Quality of Life
- COVID-19 and Mental Health
- Monoclonal and Polyclonal Antibodies Research
- Antimicrobial Resistance in Staphylococcus
- Vector-borne infectious diseases
- Viral Infections and Vectors
- Glycosylation and Glycoproteins Research
- T-cell and B-cell Immunology
- Virus-based gene therapy research
- COVID-19 and healthcare impacts
- COVID-19 Clinical Research Studies
- Mycobacterium research and diagnosis
- Sarcoma Diagnosis and Treatment
- Cytokine Signaling Pathways and Interactions
- Geriatric Care and Nursing Homes
Queen's University
2016-2025
Sunnybrook Health Science Centre
2014-2024
University of Calgary
1989-2024
McMaster University
2024
University of Toronto
2019-2024
University Health Network
2020-2024
North York General Hospital
2018-2024
Monash Medical Centre
2024
London Health Sciences Centre
2024
Monash University
2024
This document provides healthcare practitioners with information regarding the management of acute rhinosinusitis (ARS) and chronic (CRS) to enable them better meet needs this patient population. These guidelines describe controversies in bacterial (ABRS) include recommendations that take into account changes bacteriologic landscape. Recent ABRS have been released by American European groups as recently 2007, but these are either limited their coverage subject CRS, do not follow an...
With the dramatic increase in frequency of Pneumocystis carinii pneumonia associated with human immunodeficiency virus infection, there has been a need for more rapid and less invasive diagnostic techniques. Recent studies have shown that examination induced sputum can establish diagnosis P. about 55 percent cases. To assess whether recently developed indirect immunofluorescent stain using monoclonal antibodies was sensitive than Giemsa or toluidine blue O stains detecting sputum, we...
Hospital-acquired pneumonia (HAP) and ventilator-associated (VAP) are important causes of morbidity mortality, with mortality rates approaching 62%. HAP VAP the second most common cause nosocomial infection overall, but documented in intensive care unit setting. In addition, produce highest associated infection. As a result, evidence-based guidelines were prepared detailing epidemiology, microbial etiology, risk factors clinical manifestations VAP. Furthermore, an approach based on available...
We have designed cosmid vectors for rapid genomic "walking" and restriction mapping. These contain the transcription promoters from either bacteriophage SP6, T7, or T3 flanking a unique BamHI cloning site. Mammalian expression modules encoding dominant marker neomycin phosphotransferase amplifiable dihydrofolate reductase gene expressed SV40 were inserted use in transfer studies. Restriction sites enzymes Not I Sfi I, which cut mammalian DNA very infrequently, been engineered near...
Bloodstream infections are associated with substantial morbidity and mortality. Early, appropriate antibiotic therapy is important, but the duration of treatment uncertain.
We have determined the frequency of EWS fusion transcripts in a series primary Ewing's sarcomas and peripheral primitive neuroectodermal tumors cells lines. Type 1 2 EWS-Fli1 fusions were demonstrated 8 cell lines 14 patient samples. Five patients with cytogenetically characterized rearrangements chromosome 22 that did not involve 11 included these studies. A novel in-frame isoform fusing to exon Fli1 was isolated from tumor variant t(12;22;22)(q14;p1;q12) translocation. Three isoforms...
The activation of Janus kinases (JAKs) is crucial for propagation the proliferative response initiated by many cytokines. proliferation various cell lines, particularly those hematopoietic origin, also modulated mediators oxidative stress such as nitric oxide and thiol redox reagents. Herein we demonstrate that other oxidants can inhibit autokinase activity rat JAK2 in vitro , presumably through oxidation dithiols to disulfides within JAK2. reduced form most active form, oxidized inactive....
The mechanism of action prolactin (PRL) has remained obscure despite the unveiling primary structure PRL receptors.The present study demonstrates rapid receptor-mediated tyrosine phosphorylation at least three cellular proteins, designated p120, p97, and p40, in a rat T-lymphoma (Nb2-11C) as revealed by antiphosphotyrosine immunoblotting.One phosphotyrosyl co-purified with activated receptor complexes obtained using either anti-ligand or antireceptor antibodies.Furthermore, vitro incubation...
Previous results suggested that, after intranasal inoculation, mouse hepatitis virus (MHV), a neurotropic coronavirus, entered the central nervous system (CNS) via olfactory and trigeminal nerves. To prove this hypothesis, effect of interruption pathway on spread was studied using in situ hybridization. Unilateral surgical ablation prevented tract side lesion. MHV RNA could be detected, however, at distal sites operated side, indicating that well-described circuits involving anterior...
Polyadenylate-rich mRNA extracted from an established line of functional rat pituitary tumor cells (GH3) which respond to the addition thyrotropin-releasing factor with synthesis and secretion prolactin directed cell-free using a protein-synthesizing system derived wheat embryos. The products translation, when analyzed by sodium dodecyl sulfate-polyacrylamide gel electrophoresis fluorography, did not include any protein co-migrating prolactin. One prominent synthesized was precipitated...
The clinical and molecular epidemiology of health care-associated Clostridium difficile infection in nonepidemic settings across Canada has evolved since the first report virulent North American pulsed-field gel electrophoresis type 1 (NAP1) strain more than 15 years ago. objective this national, multicentre study was to describe evolving characteristics C. during a post-NAP1-epidemic period, particularly patient outcomes associated with NAP1 strain.Adult inpatients were prospectively...
KEY POINTS In an attempt to mitigate excess transmission of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), Canadian hospitals adopted "no visitor" policies during the first wave pandemic. A reflexive and almost complete restriction visitors occurred across all
Trends in COVID-19 severe outcomes have significant implications for the health care system and are key to informing public measures. However, data summarizing trends among patients hospitalized with Canada not well described.To describe during first 2 years of pandemic.Active prospective surveillance this cohort study was conducted from March 15, 2020, May 28, 2022, at a sentinel network 155 acute hospitals across Canada. Participants included adult (aged ≥18 years) pediatric 0-17...
An expression system for cell lineage ablation in transgenic mice was constructed which a modified form of the A subunit ricin, toxic lectin produced by castor bean Ricinus communis, can be expressed under direction tissue-specific regulatory signals. chimeric gene formed fusing promoter and 5'-flanking sequences lens-specific mouse alpha A-crystallin with ricin cDNA, this construction integrated into germ line mice. These animals develop profound microphthalmia severe developmental defects...
Candidemia and invasive candidiasis (C/IC) are life-threatening opportunistic infections that add excess morbidity, mortality cost to the management of patients with a range potentially curable underlying conditions. The Association Medical Microbiology Infectious Disease Canada developed evidence-based guidelines for approach diagnosis these in ever-increasing population at-risk adult health care system. Over past few years, new broader understanding epidemiology pathogenesis C/IC has...
Background. Both ethical and economics concerns have been raised with respect to the funding of drugs for rare diseases. This article reports both cost-effectiveness eculizumab treatment paroxysmal nocturnal hemoglobinuria (PNH) its associated opportunity costs. Methods. Analysis compared plus current standard care v. from a publicly funded health system perspective. A Markov model covered major consequences PNH treatment. Cost-effectiveness was assessed in terms incremental cost per life...
A combination of three monoclonal antibodies, two prepared against human and one rat Pneumocystis carinii, was used in an indirect fluorescent-antibody stain (IFA) to diagnose P. carinii both bronchoalveolar lavage lung biopsy specimens. This antibodies specific for yielded bright fluorescence cysts trophozoites. total 126 specimens from 93 patients were stained by a toluidine blue O the IFA. Forty-five (35.7%) these positive O, 43 (34.1%) There 98.4% agreement between methods, with no...
Failure to diagnose and treat hypophosphatemic rickets during childhood resulted in stunted growth progressive deformities of the lower limb. When were treated surgically, recurrent deformity non-union osteotomies developed, further major opeative procedures required remedy these complications. Treatment from early with oral phosphate vitamin D improved rate controlled progression bowleg deformity. Residual varus was corrected by osteotomy through proximal tibial metaphysis at skeletal...