A5020487524- Antimicrobial Resistance in Staphylococcus
- Bacterial Identification and Susceptibility Testing
- Clostridium difficile and Clostridium perfringens research
- Streptococcal Infections and Treatments
- Bacterial biofilms and quorum sensing
- Antibiotic Resistance in Bacteria
- Infective Endocarditis Diagnosis and Management
- Mycobacterium research and diagnosis
- Microscopic Colitis
- Viral gastroenteritis research and epidemiology
- Nosocomial Infections in ICU
- Salmonella and Campylobacter epidemiology
- Bacteriophages and microbial interactions
- Plant Pathogenic Bacteria Studies
- Mercury impact and mitigation studies
- Pharmaceutical and Antibiotic Environmental Impacts
- Antibiotic Use and Resistance
- Microbial infections and disease research
- Microbial Metabolism and Applications
- Enterobacteriaceae and Cronobacter Research
- Antimicrobial Peptides and Activities
- bioluminescence and chemiluminescence research
- Gut microbiota and health
- Antimicrobial agents and applications
- Biochemical and Structural Characterization
Public Health Agency of Canada
2015-2025
University of Manitoba
2015-2025
University of Alberta
2020
Toronto General Hospital
2020
University of Calgary
2020
Health Sciences Centre
2020
Queen Elizabeth II Health Sciences Centre
2020
University of Alberta Hospital
2020
Sunnybrook Health Science Centre
2020
Saskatchewan Health Authority
2020
Rates of colonization with livestock-associated methicillin-resistant Staphylococcus aureus (MRSA) sequence type 398 have been high for pigs and pig farmers in Canada, but prevalence rates the general human population are unknown. In this study, 5 LA-MRSA isolates, 4 which were obtained from skin soft tissue infections, identified 3,687 tested MRSA isolates persons Manitoba Saskatchewan, Canada. Further molecular characterization determined that these all contained staphylococcal cassette...
The clinical and molecular epidemiology of health care-associated Clostridium difficile infection in nonepidemic settings across Canada has evolved since the first report virulent North American pulsed-field gel electrophoresis type 1 (NAP1) strain more than 15 years ago. objective this national, multicentre study was to describe evolving characteristics C. during a post-NAP1-epidemic period, particularly patient outcomes associated with NAP1 strain.Adult inpatients were prospectively...
A mer‐lux bioreporter was used to study uptake of inorganic mercury, Hg(II), at trace concentrations by two facultatively anaerobic bacterial species, Vibrio anguillarum and Escherichia coli . Uptake Hg(II) these bacteria appeared be facilitated, rather than passive diffusion. Three lines evidence support this conclusion. First, under conditions greatly decreased compared with aerobic conditions, even though the chemical composition medium identical except for lack oxygen (i.e., no reducing...
ABSTRACT Methicillin-resistant Staphylococcus aureus (MRSA) is a pathogen that has disseminated throughout Canadian hospitals and communities. Pulsed-field gel electrophoresis of over 9,300 MRSA isolates obtained from the Nosocomial Infection Surveillance Program identified 10 epidemic strain types in Canada (CMRSA1 to CMRSA10). In an attempt determine specific genetic factors have contributed their high prevalence community and/or hospital settings, genomic content representative for each...
This study characterized cefoxitin-resistant and -susceptible Salmonella enterica serovar Heidelberg strains from humans, abattoir poultry, retail poultry to assess the molecular relationships of isolates these sources in Québec 2012. Isolates were collected as part Canadian Integrated Program for Antimicrobial Resistance Surveillance (CIPARS). All subjected antimicrobial susceptibility testing, PCR CMY-2, pulsed-field gel electrophoresis (PFGE), whole-genome sequencing (WGS). A total 113 S...
We investigated epidemiologic and molecular characteristics of healthcare-associated (HA) community-associated (CA) Clostridioides difficile infection (CDI) among adult patients in Canadian Nosocomial Infection Surveillance Program hospitals during 2015-2019. The study encompassed 18,455 CDI cases, 13,735 (74.4%) HA 4,720 (25.6%) CA. During 2015-2019, rates decreased by 23.8%, whereas CA 18.8%. was significantly associated with increased 30-day all-cause mortality as compared (p<0.01). Of...
Abstract Background Identification of transmission networks carbapenemase-producing organisms (CPO) is critical to identifying their reservoirs and limiting spread. This study aimed use whole-genome sequencing identify genomic relationships between CP Enterobacter (CP-Ent) in Ontario, Canada over a 15-year period. Methods All CP-Ent cases identified by prospective population-based surveillance the Toronto/Peel Region from first isolate 2007 2021, hospital sinks (2016-2019), wastewater...
Community-associated Clostridioides difficile infections (CA-CDI) remain a concern in Canada, comprising quarter of cases previously reported through the Canadian Nosocomial Infection Surveillance Program. Previous studies have toxigenic C. isolated from retail meat, suggesting that it may be source exposure for CA-CDI Canada. In this study, 3/219 (1.4%) pork and 0/99 (0%) beef samples tested positive difficile, which were molecularly characterized by PCR ribotyping whole-genome sequencing....
Increasing rates of methicillin-resistant Staphylococcus aureus (MRSA) infections on a global scale is major health concern. In Canada, there are 10 known epidemic types MRSA as determined by pulsed-field gel electrophoresis (PFGE). Despite the excellent discriminatory power PFGE, several disadvantages using this technique, such high degree labour intensity and inability to easily develop an typing database due subjective interpretation results.The purpose present study was determine whether...
Of 1,927 Enterococcus species isolates collected across Canada from 2007 to 2013, 80 (4.2%) were identified as vancomycin-resistant enterococci (VRE). VRE infections during this time tripled in Canadian hospitals, 1.8% 6.0% (P = 0.03). All faecium, with 90% possessing vanA. The prevalence of vanB decreased 37.5% 0% 2013 < 0.05). multidrug resistant, but 70.6%, 86.3%, and 100% susceptible doxycycline, linezolid, daptomycin, respectively.
Emergence of plasmids harbouring blaNDM-1 is a major public health concern due to their association with multidrug resistance and potential mobility. PCR was used detect from clinical isolates Providencia rettgeri (PR) Klebsiella pneumoniae (KP). Antimicrobial susceptibilities were determined using Vitek 2. The complete DNA sequence two (pPrY2001 pKp11-42) obtained 454-Genome Sequencer FLX. Contig assembly gap closures confirmed by PCR-based sequencing. Comparative analysis done BLASTn...
To compare the demographics, antimicrobial susceptibilities and molecular epidemiology of community-associated (CA) healthcare-associated (HA) methicillin-resistant Staphylococcus aureus (MRSA) in Canada.Between 2007 2011, 1266 MRSA were collected from inpatients outpatients attending tertiary-care medical centres across Canada. Susceptibility testing was performed using broth microdilution isolates characterized by spa typing PCR to detect Panton-Valentine leucocidin (PVL) gene. Detection...
Whereas the infant gut microbiome is subject of intense study, relatively little known regarding nares in newborns and during early life. This study aimed to survey typical composition diversity human anterior nare microflora for developing infants over time, explore how these correlate their primary caregivers. Single swabs were collected at five time points a one-year period each from infant-caregiver pairs. Our comprised 50 (recruited 2 weeks, post delivery) Applying chaperonin-60 (cpn60)...
This study assessed the demographic and molecular characteristics of community-associated (CA) healthcare-associated (HA) MRSA genotypes in Canadian hospitals between 2007 2016.A total 1963 were identified among 9103 Staphylococcus aureus isolates collected from inpatients outpatients presenting to tertiary-care medical centres across Canada. Antimicrobial susceptibility testing was performed by broth microdilution accordance with CLSI standards (M7 11th edition, 2018). PCR detect...
ABSTRACT Despite reports of high colonization rates ST398 livestock-associated methicillin-resistant Staphylococcus aureus (LA-MRSA) among pigs and pig farmers, the incidence LA-MRSA infection in general population Canada appears to be rare comparison that some European countries. In this study, complete genome sequence a Canadian representative isolate (08BA02176) from human postoperative surgical site was acquired compared sequenced an (S0385) Europe identify genetic traits may explain...
BackgroundThe Salmonella genomic island 1 is an integrative mobilizable element (IME) originally identified in epidemic multidrug-resistant enterica serovar Typhimurium (S. Typhimurium) DT104. SGI1 contains a complex integron, which confers various multidrug resistance phenotypes due to its genetic plasticity. Previous studies have shown that integrates site-specifically into the S. enterica, Escherichia coli, or Proteus mirabilis chromosome at 3′ end of thdF gene (attB...
We sought to analyse 10 years of longitudinal surveillance data (2007-16) from the CANWARD study and describe emerging trends in antimicrobial resistance for key bacterial pathogens across Canada.Longitudinal sites that contributed isolates every year 2007 2016 were analysed identify over time using univariate tests trend multivariate regression models account effects patient demographics.Statistically significant increases occurred proportion Escherichia coli resistant extended-spectrum...
BACKGROUND Bloodstream infection (BSI) due to methicillin-resistant Staphylococcus aureus (MRSA) is associated with considerable morbidity and mortality. OBJECTIVE To determine the incidence of MRSA BSI in Canadian hospitals identify variables increased METHODS Prospective surveillance for conducted 53 from January 1, 2008, through December 31, 2012. Thirty-day all-cause mortality was determined, logistic regression analysis used RESULTS A total 1,753 patients were identified (incidence,...
The coronavirus disease 2019 (COVID-19) pandemic has placed significant burden on healthcare systems. We compared Clostridioides difficile infection (CDI) epidemiology before and during the across 71 hospitals participating in Canadian Nosocomial Infection Surveillance Program. Using an interrupted time series analysis, we showed that CDI rates significantly increased COVID-19 pandemic.
Many studies on bioavailability of toxic metals have made the assumption that observation toxicity is evidence metal was taken into cells (i.e., "bioavailable"). A second results at high concentra tions necessary for effect are applicable to lower concentrations more commonly found in environment. These assumptions were specifically tested mercury (Hg(II)) (at 0.25−50 nM Hg) and uptake 0.005−0.015 aquatic bacterium, V. anguillarum. Toxicity measured as reduction levels constitutively...
The effects of pH on the uptake and accumulation Hg(II) by Escherichia coli were determined at trace, environmentally relevant, concentrations Hg under anaerobic conditions. was measured using inducible light production from E. HMS174 harboring a mer-lux bioreporter plasmid (pRB28). effect toxicity higher constitutive lux (pRB27) in same bacterial host. In this study, intracellular conditions both significantly enhanced with decreasing over range 8 to 5. most pronounced pHs <6, which...
Abstract Surveillance of Staphylococcus aureus infections in 3 northern remote communities Saskatchewan was undertaken. Rates methicillin-resistant were extremely high (146–482/10,000 population), and most (98.2%) caused by USA400 strains. Although prevalence has diminished the United States, this strain is continuing to predominate throughout many Canada.
Abstract Background Surveillance examining the incidence of community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA) was conducted over 8 years beginning in 2001 three health regions covering northern half Saskatchewan. The annual rate individuals reported with CA-MRSA infection these dramatically increased from 8.2 per 10,000 population (range to 4.4-10.1 10,000) 168.1 2006 43.4-230.9 10,000). To address this issue, a team community members, healthcare professionals,...
SUMMARY In this case-control study, cases [community-associated methicillin-resistant Staphylococcus aureus (CA-MRSA), n =79] and controls methicillin-susceptible S. (CA-MSSA), =36] were defined as a laboratory-confirmed infection in patient with no previous hospital-associated factors. Skin soft tissue the predominant sites of infection, both for (67·1%) (55·6%). Most (79·7%) (77·8%) aged <30 years. Investigations did not reveal any significant statistical differences acquiring CA-MRSA...