A5005922936- Genomics and Chromatin Dynamics
- Erythropoietin and Anemia Treatment
- Hormonal and reproductive studies
- Cancer-related gene regulation
- Sepsis Diagnosis and Treatment
- Enzyme Structure and Function
- Chronic Kidney Disease and Diabetes
- Ubiquitin and proteasome pathways
- Aging, Elder Care, and Social Issues
- Immune Response and Inflammation
- Phagocytosis and Immune Regulation
- Erythrocyte Function and Pathophysiology
- Biosimilars and Bioanalytical Methods
- Acute Kidney Injury Research
- Protein Kinase Regulation and GTPase Signaling
- Bacterial Identification and Susceptibility Testing
- Cell Adhesion Molecules Research
- RNA modifications and cancer
- Fungal and yeast genetics research
- Cardiac Arrest and Resuscitation
- Cell death mechanisms and regulation
- RNA and protein synthesis mechanisms
- S100 Proteins and Annexins
- Mechanisms of cancer metastasis
- Cancer therapeutics and mechanisms
Sunnybrook Health Science Centre
2024
North York General Hospital
2024
Monash Medical Centre
2024
University Health Network
2024
University of Toronto
2024
London Health Sciences Centre
2024
Monash University
2024
Toronto East General Hospital
2024
Auckland City Hospital
2024
Institut National de la Recherche Scientifique
2012-2023
Bloodstream infections are associated with substantial morbidity and mortality. Early, appropriate antibiotic therapy is important, but the duration of treatment uncertain.
Oncogenic stress induces expression of the alternate reading frame (Arf) tumor suppressor protein. Arf then stabilizes p53, which leads to cell cycle arrest or apoptosis. The mechanisms that distinguish both outcomes are incompletely understood. In this study, we show interacts with Myc-associated zinc finger protein Miz1. Binding disrupts interaction Miz1 its coactivator, nucleophosmin, sumoylation Miz1, and facilitates assembly a heterochromatic complex contains Myc trimethylated H3K9 in...
Abstract Specific heterodimerization plays a crucial role in the regulation of biology cell. For example, specific between b‐HLH‐LZ transcription factors c‐Myc and Max is prerequisite for transcriptional activity that leads to cell growth, proliferation tumorigenesis. On other hand, Mad proteins can compete with Max. The Mad/Max heterodimer antagonizes effect c‐Myc/Max heterodimer. In this contribution, we have focused on domains using CD NMR. While are found preferentially form heterodimer;...
The Myc/Mad/Max network of dimeric basic region−helix−loop−helix−leucine zipper (b-HLH-LZ) transcription factors bind to enhancer box sequences (E-box) in the promotors a large set genes that control cell metabolism, proliferation, and differentiation. Max (Myc-associated factor X) is obligate heterodimerization partner Myc Mad proteins. On other hand, only member family capable forming stable homodimer. As part transcriptional regulation mechanism, Myc/Max Mad/Max heterodimers homodimers...
According to the World Anti‐Doping Agency (WADA) technical document for erythropoiesis stimulating agents (ESA) analysis (TD2014EPO), double‐blotting of serum/plasma samples is mandatory all by isoelectric focusing (IEF) and confirmation procedures (CP) performed SDS‐PAGE or SAR‐PAGE. The goal prevent potential cross‐reactions secondary antibody with remaining proteins in purified samples. To this end, we have developed an immunopurification method ESA using a combination streptavidin‐coated...
To elucidate the mechanisms that regulate human neutrophil delivery in vivo, as well lead to observed reduction polymorphonuclear (PMN) remote sites septic patients.Alterations PMN chemoattractant receptor expression and chemotactic function vivo were evaluated two distinct experiments: exudate PMNs (PMNs have undergone transmigration skin window blisters controls) (circulating from patients intensive care unit) both separately compared with control circulating PMNs.Exudate displayed...
The b-HLH-LZ family of transcription factors contains numerous proteins including the Myc and Mad families proteins. Max heterodimerizes with other members to bind E-Box DNA sequence in target gene promoters. is only protein this network that recognizes binds sequences as a homodimer vitro represses genes vivo. Key information such structure p21 Max, complete product, its KD absence are still unknown. Here, we report characterization secondary quaternary structures, dimerization binding...
c-Myc must heterodimerize with Max to accomplish its functions as a transcription factor. This specific heterodimerization occurs through the b-HLH-LZ (basic region, helix 1-loop-helix 2-leucine zipper) domains. In fact, many studies have shown that (c-Myc'SH) preferentially forms heterodimer (Max'SH). The primary mechanism underlying lies on destabilization of both homodimers and formation more stable heterodimer. this regard, it has been widely reported c-Myc'SH low solubility...
Recombinant erythropoietin (rhEPO) has been misused for over two decades by athletes, mainly but not only in endurance sports. A direct rhEPO detection method urine isoelectric focusing (IEF) was introduced 2000, the emergence of third‐generation erythropoiesis‐stimulating agents and so‐called biosimilar rhEPOs, together with sensitivity human endogenous EPO (huEPO) pattern to enzymatic activities its modification following short strenuous exercise, prompted development a complementary test...
The aim of our study is to describe the changes in urinary and serum levels novel biomarkers after gadolinium contrast administration patients with normal renal function.We measured four 28 volunteers: interleukin-18 (IL-18), N-acetyl-glucosaminidase (NAG), neutrophil gelatinase-associated lipocalin, cystatin C. Urinary samples were collected at 0, 3, 24 hours following administration.Baseline creatinine was 57.8 ± 34.5 µmol/L remained stable. IL-18 increased significantly three (10.7 vs....
Abstract The presence of erythropoiesis stimulating agents (ESAs) in the urine samples collected from athletes is detected using traditional Western blotting following either size‐based separation (SDS/SAR‐PAGE) or isoelectric focusing (IEF). Although there an important testing effort, little doubt that ESAs are still abused sports and reducing costs tests might increase number improve deterrence. capillary electrophoresis system developed by Protein Simple may be useful to this end. This...
Abstract Introduction The percentage of circulating reticulocytes (RET%) is a useful marker blood doping in the context Athlete Biological Passport (ABP). viability ABP depends on comparability sample data obtained across multiple laboratories for given athlete. With recent introduction different technology measurement reticulocytes, goal this study was to compare currently employed Sysmex XT/XE analyzers recently introduced XN analyzer. Methods RET% differences were searched two independent...
Acute Renal Failure (ARF) remains a common and significant problem in modern medicine. The epidemiology causes of ARF vary according to the clinical setting; intensive care units it is estimated that incidence ranges from 10%–25%,1,2 with acute tubular necrosis (ATN) accounting for about 75% cases.3 Recent data BEST kidney investigators has worldwide prevalence likely require renal replacement therapy (RRT) amongst ICU patients be as high 5.7%, associated hospital mortality rate.4 Despite...
Mad1 is a member of the Mad family. This family part larger Myc/Max/Mad b-HLH-LZ eukaryotic transcription-factor network. forms specific heterodimer with Max and acts as transcriptional repressor when bound to an E-box sequence (CACGTG) found in promoter c-Myc target genes. cannot form complex DNA by itself under physiological conditions. A global model for molecular recognition has emerged which homodimer destabilized Mad/Max favored. The detailed structural determinants responsible remain...
Activin receptor type IIA and IIB fusion protein have been designed to sequester circulating molecules of the transforming growth factor-β (TGF-β) superfamily inactivate their actions. Members this reported as essential regulators erythropoiesis by triggering formation activated ternary complexes containing different combinations I II receptors, which can limit RBC production accelerating erythroid differentiation inhibiting progenitor expansion. The recent approval Luspatercept for...
This article looks at the survival strategies used in traditional societies by households that were greatly disrupted death of father, usually their principal provider. Research is based on data from two parish censuses (1716 and 1744) city Québec describe composition urban households. Data Registre de la Population du Ancien (RPQA), register population before 1800, also to complement information required. Depending a mother after father's death, it appears could maintain nuclear structure,...
Naud (Jean-Francois), Desjardins (Bertrand), Charbonneau (Hubert).- Los componentes de la paternidad biologica bajo regimen fecundidad natural: el ejemplo del Quebec antiguo El articulo presenta un analisis descriptivo en una pobla- cion natural. Se analiza poblacion Canada frances, particular segunda generacion canadienses nacimiento. datos proce- den Registro (Programa Investigacion demo- grafia historica, Universidad Montreal). analisis, realizado a nivel individuos, se basa importancia e...
A 62-year-old man presented to the emergency department with massive hemoptysis. After bronchial artery embolization, he developed ischemic myelitis, a rare complication in this setting for which no specific therapy is currently recommended. The symptoms were managed lumbar drainage of cerebrospinal fluid and blood pressure augmentation therapy. To our knowledge, first report using treatment anterior medullary ischemia after embolization was associated neurological recovery.