A5082953593- Immune cells in cancer
- RNA modifications and cancer
- Epigenetics and DNA Methylation
- Neutrophil, Myeloperoxidase and Oxidative Mechanisms
- Immune Response and Inflammation
- Cell Adhesion Molecules Research
- Cancer Research and Treatments
- Nanoplatforms for cancer theranostics
- Cancer-related gene regulation
- Chemokine receptors and signaling
- Lung Cancer Treatments and Mutations
- Cancer Cells and Metastasis
- Sepsis Diagnosis and Treatment
- Helicobacter pylori-related gastroenterology studies
- Cancer Immunotherapy and Biomarkers
- S100 Proteins and Annexins
- Trauma, Hemostasis, Coagulopathy, Resuscitation
- Extracellular vesicles in disease
- Cancer, Stress, Anesthesia, and Immune Response
- Phagocytosis and Immune Regulation
- Respiratory Support and Mechanisms
- Traumatic Brain Injury and Neurovascular Disturbances
- Neonatal Respiratory Health Research
- Inflammatory Biomarkers in Disease Prognosis
- Macrophage Migration Inhibitory Factor
McGill University Health Centre
2014-2024
McGill University
2012-2024
University of Calgary
2012
Committee on Publication Ethics
2003
The majority of patients with cancer undergo at least one surgical procedure as part their treatment. Severe postsurgical infection is associated adverse oncologic outcomes; however, the mechanisms underlying this phenomenon are unclear. Emerging evidence suggests that neutrophils, which function first line defense during infections, facilitate progression. Neutrophil extracellular traps (NETs) neutrophil-derived DNA webs released in response to inflammatory cues trap and kill invading...
Although circulating neutrophils are associated with distant metastasis and poor outcome in a number of epithelial malignancies, it remains unclear whether play an active causal role the metastatic cascade. Using vivo models metastasis, we found that promote cancer cell adhesion within liver sinusoids and, thereby, influence metastasis. Neutrophil depletion before inoculation resulted decreased gross metastases intrasplenic model This effect was reversed when inflamed were co-inoculated...
Infectious complications resulting from resection of colorectal cancer (CRC) elevates the risk recurrence and metastasis, but reason for this relationship is unknown. Defining mechanisms responsible may offer opportunities to improve outcomes in a majority patients whose tumors are resected as part their therapy. The complex formed between Toll receptor TLR4 myeloid differentiation factor MD2 defines major cell surface lipopolysaccharide (LPS), gram-negative bacterial antigen that has been...
Despite advances in cancer treatment, metastasis remains today the main cause of death. Local control through complete surgical resection primary tumor continues to be a key principle treatment. However, interventions themselves lead adverse oncologic outcomes and are associated with significantly increased rates metastasis. Neutrophils release neutrophil extracellular traps (NETs) response infections were shown able capture circulating cells, doing so, support development metastatic...
Targeting the dynamic tumor immune microenvironment (TIME) can provide effective therapeutic strategies for cancer. Neutrophils are predominant leukocyte population in mice and humans, mounting evidence implicates these cells during growth metastasis. Neutrophil extracellular traps (NETs) networks of neutrophil DNA fibers that capable binding to support metastatic progression. Here, we demonstrate circulating NET levels elevated advanced esophageal, gastric, lung cancer patients compared...
Neutrophil Extracellular Traps (NETs) have been recently identified as part of the neutrophil's antimicrobial armamentarium. Apart from their role in fighting infections, recent research has demonstrated that they may be involved many other disease processes, including cancer progression. Isolating purified NETs is a crucial element to allow study these functions. In this video, we demonstrate simplified method cell free NET isolation human whole blood using readily available reagents....
Neutrophils promote tumor growth and metastasis at multiple stages of cancer progression. One mechanism through which this occurs is via release neutrophil extracellular traps (NETs). We have previously shown that NETs trap cells in both the liver lung, increasing their adhesion following postoperative complications. Multiple studies since play a role progression metastasis. are composed nuclear DNA-derived web-like structures decorated with neutrophil-derived proteins. However, it unknown...
Objective To evaluate the in vivo effects of hypertonic saline (HTS) resuscitation on interactions endothelial cells (ECs) and polymorphonuclear neutrophils (PMNs) vascular permeability after hemorrhagic shock. Summary Background Data The PMN has been implicated pathogenesis EC damage organ injury following Compared to Ringer's lactate (RL), HTS diminishes adhesion molecule expression sequestration PMNs. Methods In a murine model shock (50 mmHg for 45 minutes followed by resuscitation) using...
Lymph nodes (LNs) are frequently the first sites of metastasis. Currently, only prognostic LN assessment is determining metastatic status. However, there evidence suggesting that metastasis facilitated by formation a pre-metastatic niche induced tumour derived extracellular vehicles (EVs). Therefore, it important to detect and modify environmental changes. Earlier work has demonstrated neutrophil traps (NETs) can sequester promote distant Here, we confirmed NETs associated with reduced...
Background Hypertonic saline (HTS) attenuates polymorphonuclear neutrophil (PMN)-mediated tissue injury after hemorrhagic shock. We hypothesized that HTS resuscitation reduces early in vivo endothelial cell (EC)-PMN interactions and late lung PMN sequestration a two-hit model of shock followed by mimicked infection. Methods Thirty-two mice were hemorrhaged (40 mm Hg) for 60 minutes then given intratracheal lipopolysaccharide (10 μg) 1 hour with shed blood either (4 mL/kg 7.5% NaCl) or...
Surgery is required for the curative treatment of lung cancer but associated with high rates postoperative pneumonias predominantly caused by gram negative bacteria. Recent evidence suggests that these severe infectious complications may decrease long term survival after hospital discharge via recurrence, mechanism unclear. Lung cells have recently been demonstrated to express Toll‐like receptors (TLR) mediate pathogen recognition. We hypothesized incubation non‐small cell (NSCLC)...
Abstract While emerging data suggest nucleotide oligomerization domain receptor 1 (NOD1), a cytoplasmic pattern recognition receptor, may play an important and complementary role in the immune response to bacterial infection, its cancer metastasis is entirely unknown. Hence, we sought determine effects of NOD1 on metastasis. expression paired human primary colon cancer, murine cells were determined using immunohistochemistry immunoblotting (WB). Clinical significance was assessed TCGA...
Neutrophil Extracellular Traps (NETs) have been recently identified as part of the neutrophil's antimicrobial armamentarium. Apart from their role in fighting infections, recent research has demonstrated that they may be involved many other disease processes, including cancer progression. Isolating purified NETs is a crucial element to allow study these functions. In this video, we demonstrate simplified method cell free NET isolation human whole blood using readily available reagents....
Surgical resection of early stage nonsmall cell lung cancer (NSCLC) is necessary for cure. However, rates postoperative bacterial pneumonias remain high and may confer an increased risk metastasis. Toll-like receptors (TLRs) mediate the inflammatory cascade by recognizing microbial products at surface numerous types in lung; however, little known about how host TLRs influence NSCLC TLR2 recognizes gram-positive wall components activating innate immunity. We demonstrate that lower respiratory...
IntroductionSurgery is essential for cure of early-stage non-small cell lung cancer (NSCLC). Rates postoperative bacterial pneumonias, however, remain high, and clinical data suggests that post-operative infectious complications confer an increased risk metastasis. Toll-like receptors (TLRs) mediate the inflammatory response to infection by recognizing evolutionarily conserved structures at surface numerous pulmonary types; yet, little known about how host TLR activation influences NSCLC...
To elucidate the mechanisms that regulate human neutrophil delivery in vivo, as well lead to observed reduction polymorphonuclear (PMN) remote sites septic patients.Alterations PMN chemoattractant receptor expression and chemotactic function vivo were evaluated two distinct experiments: exudate PMNs (PMNs have undergone transmigration skin window blisters controls) (circulating from patients intensive care unit) both separately compared with control circulating PMNs.Exudate displayed...
<h3>Objective:</h3> To determine whether the inflammatory microenvironment primes neutrophils for increased microbicidal activity. <h3>Design:</h3> In vitro studies of host defense were performed on surgical patients. <h3>Setting:</h3> A tertiary care, university hospital. <h3>Patients:</h3> volunteer sample hospitalized preoperative, noninfected <h3>Intervention:</h3> Exudative collected from skin-blister chambers and functionally compared with circulating neutrophils. <h3>Methods:</h3>...
<h3>Objectives</h3> To test the hypothesis that loss of polymorphonuclear neutrophil tumor necrosis factor α (TNF-α) receptors during transmigration renders exudate refractory to TNF-α–mediated stimulation apoptosis; and investigate surface expression Fas on both circulating neutrophils. <h3>Design</h3> A prospective cohort study. <h3>Setting</h3> Surgical laboratory a tertiary care hospital. <h3>Participants</h3> Twenty-one healthy human volunteers. <h3>Interventions</h3> All subjects had...
Background: The polymorphonuclear neutrophil (PMN) has been implicated in the pathogenesis of endothelial cell (EC) damage and organ injury following hemorrhagic shock. Pentastarch (PTS), a low substituted medium molecular weight (MW) colloid, improves hemodynamics hypovolemic shock cardiac surgery. No data exist comparing immunomodulation PTS Ringer's lactate (RL) on activation PMN vivo. Methods: Using an vivo murine model (blood withdrawal to maintain 50 mmHg × 45 min), circulating were...
Targeting the dynamic tumor immune microenvironment can provide effective therapeutic strategies for cancer. Neutrophils (polymorphonuclear neutrophils, PMNs) are predominant circulating leukocyte population in humans and vital to fight infection. Despite mounting evidence that PMNs promote progression, depleting is not a viable option. Neutrophil extracellular traps (NETs) networks of neutrophil DNA fibers capable trapping cells promoting their growth metastasis. NETs therefore be...
Abstract Lymph nodes (LNs) are frequently the first sites of metastasis. Currently, only prognostic LN assessment is determining metastasis status. However, there evidence suggesting that facilitated by a pre-metastatic niche induced tumour derived extracellular vehicles (EVs). Therefore, it important to detect and modify environmental changes. We have previously reported neutrophil traps (NETs) can sequester promote distant Here, we confirmed NETs associated with reduced patient survival....