A5091648479- Trace Elements in Health
- Heavy Metal Exposure and Toxicity
- Retinal Development and Disorders
- Mercury impact and mitigation studies
- Selenium in Biological Systems
- Photoreceptor and optogenetics research
- Immunotherapy and Immune Responses
- Neuroscience and Neural Engineering
- Metal complexes synthesis and properties
- X-ray Spectroscopy and Fluorescence Analysis
- Cell Adhesion Molecules Research
- Drug Transport and Resistance Mechanisms
- Ion Transport and Channel Regulation
- Arsenic contamination and mitigation
- Advanced Chemical Physics Studies
- Pancreatic function and diabetes
- Healthcare and Venom Research
- Polymer Surface Interaction Studies
- SARS-CoV-2 and COVID-19 Research
- Photochromic and Fluorescence Chemistry
- Immune Cell Function and Interaction
- T-cell and B-cell Immunology
- Plant nutrient uptake and metabolism
- Analytical chemistry methods development
- Cellular Mechanics and Interactions
California Institute for Biomedical Research
2023-2025
Scripps Institution of Oceanography
2023-2025
University of Saskatchewan
2013-2024
University of Pennsylvania
2014-2022
Rostov Research Institute of Microbiology and Parasitology
2022
Saskatoon Medical Imaging
2020
University of Florida
2008-2019
Gainesville Obstetrics & Gynecology
2015
Lomonosov Moscow State University
2003-2007
Significance We restored visual function to animal models of human blindness using a chemical compound that photosensitizes mammalian ion channel. Virus-mediated expression this light sensor in surviving retinal cells blind mice responses vitro, reanimated innate avoidance, and enabled learned visually guided behavior. The treatment also the retina dogs. Patients might benefit from would need have intact ganglion cell nerve fiber layers. In general, these are patients diagnosed with...
Abstract Magnesium has recently received an increased amount of interest due to its potential use in biodegradable implant applications. The rapid degradation conventional Mg is, however, a major limitation that needs be addressed the design these materials, along with consideration toxicity selection alloying elements. In this study, five alloys Mg‐ x Ca‐ y Sr system ( = 0.5–7.0 wt %; 0.5–3.5 %) were prepared and characterized for their suitability as degradable orthopedic materials. using...
Antigen specificity is a primary goal in developing curative therapies for autoimmune disease. Dendritic cells (DCs), as the most effective antigen presenting body, represent key target to mediate restoration of antigen-specific immune regulation. Here, we describe an injectable, dual-sized microparticle (MP) approach that employs phagocytosable ∼1 μm and nonphagocytosable ∼30 MPs deliver tolerance-promoting factors both intracellularly extracellularly, well type 1 diabetes autoantigen,...
Selenoenzymes, containing a selenocysteine (Sec) residue, fulfill important roles in biology. The mammalian thioredoxin reductase selenoenzymes are key regulators of antioxidant defense and redox signaling inhibited by methylmercury species the gold-containing drug auranofin. It has been proposed that such inhibition is mediated metal binding to Sec enzyme. However, direct structural observations these classes inhibitors have few date. Here we therefore used extended X-ray absorption fine...
Human copper transporters ATP7B (Wilson's disease protein) and ATP7A (Menkes' have been implicated in tumour resistance to cisplatin, a widely used anticancer drug. Cisplatin binds the copper-binding sites N-terminal domain of ATP7B, this binding may be an essential step cisplatin detoxification involving ATPases. In present study, we demonstrate that related platinum drug carboplatin produce same adduct following reaction with MBD2 [metal-binding (repeat) 2], where is bound side chains...
Industrial release of mercury into the local Minamata environment with consequent poisoning communities through contaminated fish and shellfish consumption is considered classic case environmental poisoning. However, species in factory effluent has proved controversial, originally suggested as inorganic, more recently methylmercury species. We used newly available methods to re-examine cerebellum historic Cat 717, which was fed mixed food confirm source. Synchrotron high-energy-resolution...
Regenerative therapies aimed at replacing photoreceptors are a promising approach for the treatment of otherwise incurable causes blindness. However, such still face significant hurdles, including need to improve subretinal delivery and long-term survival rate transplanted cells, promote sufficient integration into host retina. Here, we successfully delivered in vitro-derived human photoreceptor precursor cells (PRPCs; also known as immature photoreceptors) space seven normal three...
The causative agent of Covid-19 is the SARS-CoV-2 virus. Initiation cell entry by critically dependent upon binding spike protein to angiotensin-converting enzyme 2 (ACE2, EC 3.4.17.23). mechanism receptor domain ACE2 among most intensively studied infection mechanisms any pathogen, including a very large number structural studies. membrane-associated zinc carboxypeptidase, comprising three domains, protease domain, neck and membrane-spanning α-helical domain. In addition its role as also...
Wilson disease ATPase (ATP7B) has been implicated in the resistance of cancer cells to cisplatin. Using a simple vivo assay bacterial culture, present study we demonstrate that ATP7B can confer cisplatin by sequestering drug its N-terminal metal-binding domain without active extrusion from cell. Expression protein fragment containing four MBRs (metal-binding repeats) (MBR1-4) protects toxic effects One MBR1-4 molecule binds up three molecules at copper-binding sites MBRs. The findings...
Selenium is found to be highly concentrated in zebrafish pigment (melanin) containing tissues especially for the organic selenium treatments, with lower concentrations eye lens, yolk sac and heart.
Alzheimer's disease (AD) is the main cause of age-related dementia and currently affects approximately 5.7 million Americans. Major brain changes associated with AD pathology include accumulation amyloid beta (Aβ) protein fragments formation extracellular plaques. Redox-active metals mediate oligomerization Aβ, resultant metal-bound oligomers have been implicated in putative harmful, reactive species that could contribute to observed oxidative damage. In isolated plaque cores, Cu(II) bound...
Mercury is ubiquitous in the environment, with rising levels due to pollution and climate change being a current global concern. Many mercury compounds are notorious for their toxicity, potential of organometallic devastating effects on structures functions central nervous system particular Chronic exposure human populations low methylmercury occurs through consumption fish other seafood, although health consequences, if any, from this remain controversial. We have used high energy...
Although Alzheimer's disease (AD) was first described over a century ago, it remains the leading cause of age-related dementia. Innumerable changes have been linked to pathology AD; however, there much discord regarding which might be initial disease. The "amyloid cascade hypothesis" proposes that amyloid β (Aβ) peptide is central pathology, supported by elevated Aβ levels in brain before development symptoms and correlations burden with cognitive impairment. "metals role for metal ions such...
Mediator of ERBB2-driven cell motility 1 (MEMO1) is an evolutionary conserved protein implicated in many biological processes; however, its primary molecular function remains unknown. Importantly, MEMO1 overexpressed types cancer and was shown to modulate breast metastasis through altered motility. To better understand the cells, we analyzed genetic interactions using gene essentiality data from 1028 lines found multiple iron-related genes exhibiting relationships with MEMO1. We...
Antibacterial coating approaches are being investigated to modify implants reduce bacterial adhesion and viability in order implant-associated infection. Nanostructured materials possess unique surface properties, nanotopographic surfaces have been reported modulate adhesion. Zinc oxide (ZnO) films presenting well-controlled nanorod structures recently developed. To assess the efficacy of ZnO as an anti-bacterial coating, we evaluated viability, compared sputtered substrates (a relatively...