A5040156560- Infective Endocarditis Diagnosis and Management
- Streptococcal Infections and Treatments
- Bacterial Identification and Susceptibility Testing
- Antimicrobial Resistance in Staphylococcus
- Bacteriophages and microbial interactions
- Genomics and Phylogenetic Studies
- Burkholderia infections and melioidosis
- Identification and Quantification in Food
- Pneumonia and Respiratory Infections
- Diphtheria, Corynebacterium, and Tetanus
- Viral Infections and Vectors
- Molecular Biology Techniques and Applications
- Brucella: diagnosis, epidemiology, treatment
- Connective tissue disorders research
- Vector-Borne Animal Diseases
- Microbial Metabolism and Applications
- Vector-borne infectious diseases
- Chemical Looping and Thermochemical Processes
- Pleural and Pulmonary Diseases
- Bacterial Infections and Vaccines
- Actinomycetales infections and treatment
- DNA and Nucleic Acid Chemistry
- Rabies epidemiology and control
- Syphilis Diagnosis and Treatment
- Bartonella species infections research
Region Zealand
2012-2023
Zealand University Hospital Køge
2022
Slagelse Hospital
2012-2020
Ringsted Sygehus
2012-2018
Statens Serum Institut
2006-2015
Odense University Hospital
2007-2012
Matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry with a Bruker Daltonics microflex LT system was applied to 90 well-characterized catalase-negative, Gram-positive cocci not belonging the streptococci or enterococci. Biotyper version 2.0.43.1 software used singly in combination database extension generated this study 51 collection strains from 16 genera. Most were identified by using both databases individually, and some only applying combined database....
Certain viruses such as tailed bacteriophages and herpes simplex virus package double-stranded DNA into empty procapsids via powerful, ring-shaped molecular motors. High resolution structures force measurements on the packaging motor of bacteriophage φ29 revealed that its five ATPase subunits coordinate ATP hydrolysis with each other to maintain proper cyclic sequence translocation steps about ring. Here, we explore how regulates by timing key events, namely binding/hydrolysis gripping,...
Matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF MS) is currently being introduced for the rapid and accurate identification of bacteria. We describe 2 MALDI-TOF MS cases – 1 directly on spinal fluid grown Rapidly obtained results had great value continued treatment elucidation exposure.
Average nucleotide identity analysis, based on whole genome sequences of 115 strains previously identified as Aerococcus urinae, an emerging uropathogen, discriminates at least six unique genomic taxa. The analysis affords clearer species boundaries over 16S rRNA gene sequencing and traditional phenotypic approaches for the identification phylogenetic organization species. newly described can be differentiated by matrix-assisted laser desorption ionization time-of-flight protein signatures....
Nonhemolytic streptococci (NHS) cause serious infections, such as endocarditis and septicemia. Many conventional phenotypic methods are insufficient for the identification of bacteria in this group to species level. Genetic analysis has revealed that single-gene is all bacteria. The aim present study was establish a method based on sequence 16S-23S intergenic spacer (ITS) region partial gdh gene identify clinical relevant NHS Sequence ITS performed with 57 reference or strains. Satisfactory...
Aerococcus sanguinicola is a Gram-positive coccus first described in 2001. Infections humans are rare but the use of 16S rRNA gene sequencing and improved phenotypic methods has facilitated identification A. sanguinicola. We report here 6 cases bacteraemia, 2 which were associated with infective endocarditis. Most patients elderly (median age 70 y) had underlying neurological disorders including dementia, cerebral degeneration, myelomeningocele. The primary focus infection was urinary tract...
Purpose.Streptococcus oralis and Streptococcus mitis belong to the Mitis group, which are mostly commensals in human oral cavity. Even though S. commensals, they can be opportunistic pathogens causing infective endocarditis. A recent taxonomic re-evaluation of group has embedded species tigurinus dentisani into as subspecies. In this study, distribution virulence factors that contribute bacterial immune evasion, colonization adhesion was assessed clinical strains (subsp. oralis, subsp....
Introduction Lyme borreliosis (LB) is the most common tick transmitted disease in Europe. The diagnosis of LB today based on patient´s medical history, clinical presentation and laboratory findings. diagnostics are mainly antibody detection, but certain conditions molecular detection by polymerase chain reaction (PCR) may serve as a complement. Aim purpose this study was to evaluate analytical sensitivity, specificity concordance eight different real-time PCR methods at five laboratories...
Cardiobacterium valvarum is a newly recognized human pathogen related to infective endocarditis. species are, however, only rarely the aetiology of An endocarditis case presented and, additionally, phenotypic and phylogenetic comparison further 10 collection strains, representing two within genus, was performed. C. isolated from blood DNA present in valvular tissue (partial 16S rRNA gene analysis) 64-year-old man with mitral valve, rupture chordae prolapse pulmonary valves addition...
Abstract Streptococcus gordonii and sanguinis belong to the Mitis group streptococci, which mostly are commensals in human oral cavity. Though they commensals, can escape their niche cause infective endocarditis, a severe infection with high mortality. Several virulence factors important for development of endocarditis have been described these two species. However, background how commensal bacteria, some cases, become pathogenic is still not known. To gain greater understanding mechanisms...
A polymerase chain reaction was used to identify Brucella species isolated from humans in Denmark. Consecutive analysis of referred bacteria and re-examination historical isolates identified all as melitensis. Multiple-locus variable number tandem repeat (MLVA) placed the previously defined 'East Mediterranean' B. melitensis group.
Studies on the value of culture-independent molecular identification bacteria in cardiac valves are mostly restricted to comparing agreement what is obtained by culture number identified culture-negative cases. However, evaluation usefulness direct should also address weaknesses, their relevance given setting, and possible improvements. In this study from 56 Danish patients referred for surgery infective endocarditis were analysed microscopy as well PCR targeting part bacterial 16S rRNA gene...
Strains belonging to the genus Aerococcus are causative agents of human and animal infections, including urogenital bacteremia/septicemia, infective endocarditis. This study reports first fully closed complete genome sequences six type strains using a combination Illumina HiSeq PacBio sequencing technologies.
A Caulobacter sp. isolate was recovered from the dialysis fluid of a patient undergoing peritoneal dialysis. Bacterial identification included electron microscopy and 16S rDNA sequencing. To our knowledge, this is first report human infection. Special growth requirements suggest that spp. may be overlooked in clinical microbiology laboratory.
The use of broad range PCR and DNA sequencing bacterial 16S ribosomal RNA genes for routine diagnostics infections was evaluated. Here, the results from more than 2600 analyses during a 6-year period (2003-2009) are presented. Almost half samples were joints bones, second most frequent origin central nervous system. Overall, 26% all positive identification obtained in 80% PCR-positive by subsequent sequencing. Ambiguous species noticed among non-haemolytic streptococci, especially within...
A correct identification of Streptococcus pseudopneumoniae is a prerequisite for investigating the clinical impact bacterium. The has traditionally relied on phenotypic methods. However, these traits have been shown to be unreliable, with some S. strains giving conflicting results. Therefore, sequence-based methods increasingly used In this study, we 64 strains, 59 pneumoniae 22 mitis 24 oralis 6 infantis and 1 peroris strain test capability three single genes (rpoB, gyrB, recA), two...
The results of partial polymerase chain reaction (PCR) amplification the bacterial 16S rRNA gene and subsequent DNA sequencing clinical samples from children are described. In 13 out 62 samples, bacteria likely to be cause infection was identified. vast majority (11/13) with significant pathogen culture specimen had been negative. Antibiotics given in all cases except for three prior sampling. PCR is a valuable supplementary tool establishing infections when
The species that are catalase-negative, gram-positive cocci and not belonging to streptococci or enterococci have become increasingly well characterized the number of taxonomic entities steadily growing based on molecular studies.This complicates their identification.16S-23S Intergenic Spacer (ITS) region sequence analysis has proven be a useful tool for identification genera Streptococcus Enterococcus.This study investigated possibility using ITS as common within Aerococcus, Abiotrophia,...
Development of rapid and sensitive techniques for detection B. pseudomallei is an important aim public health.Here we report a specific SYBR Green-based real time PCR targeting species toxin gene.The assay able to detect 1-2 genome equivalents pseudomallei.