A5030417882- Vector-borne infectious diseases
- Viral Infections and Vectors
- Vector-Borne Animal Diseases
- Celiac Disease Research and Management
- Mosquito-borne diseases and control
- Microscopic Colitis
- Respiratory viral infections research
- Galectins and Cancer Biology
- Monoclonal and Polyclonal Antibodies Research
- Hematopoietic Stem Cell Transplantation
- Influenza Virus Research Studies
- Autoimmune Bullous Skin Diseases
- T-cell and B-cell Immunology
- Immune Response and Inflammation
- Viral gastroenteritis research and epidemiology
- Immunotherapy and Immune Responses
- Bartonella species infections research
- Peptidase Inhibition and Analysis
- Viral Infections and Immunology Research
- Dermatological diseases and infestations
- Immune Cell Function and Interaction
- Protein purification and stability
- Transgenic Plants and Applications
- Yersinia bacterium, plague, ectoparasites research
- Helicobacter pylori-related gastroenterology studies
Sørlandet Sykehus
2012-2024
Hospital of Southern Norway
2017-2018
Oslo University Hospital
1991-2007
University of Oslo
1998-2007
Agder Research
2007
Stanford University
2004
Institute of Immunology
2001
The great majority of patients that are intolerant wheat gluten protein due to celiac disease (CD) human histocompatibility leukocyte antigen (HLA)-DQ2+, and the remaining few normally express HLA-DQ8. These two class II molecules chiefly responsible for presentation peptides gluten-specific T cells found only in gut CD but not controls. Interestingly, tissue transglutaminase (tTG)-mediated deamidation gliadin plays an important role recognition this food by intestinal cells. Here we have...
Celiac disease, also known as celiac sprue, is a gluten-induced autoimmune-like disorder of the small intestine, which strongly associated with HLA-DQ2. The structure DQ2 complexed an immunogenic epitope from gluten, QLQPFPQPELPY, has been determined to 2.2-Å resolution by x-ray crystallography. glutamate at P6, formed tissue transglutaminase-catalyzed deamidation, important anchor residue it participates in extensive hydrogen-bonding network involving Lys-β71 DQ2. gluten peptide–DQ2 complex...
Tetramers of MHC–peptide complexes are used for detection and characterization antigen-specific T cell responses, but they require knowledge about both antigenic peptide the MHC restriction element. The successful application these reagents in human diseases involving CD4 + cells is limited. Celiac disease, an intestinal inflammation driven by mucosal recognizing wheat gluten peptides context disease-associated HLA-DQ molecules, ideal model to test potential clinical use reagents. We...
DQ2 confers susceptibility to celiac disease (CD) and intestinal CD4(+) T cells of DQ2(+) CD patients preferentially recognize deamidated gliadin peptides. This modification can be mediated by tissue transglutaminase (tTG). We have investigated what role the tTG-modified residues play in binding cell presentation using a model gamma-gliadin peptide (residues 134 - 153). Treatment this with tTG resulted deamidation Gln at positions 140, 148 150. Two these act as anchors position P7 (148) P9...
Abstract Gluten-specific T cells in the small intestinal mucosa are thought to play a central role pathogenesis of celiac disease (CD). The vast majority these recognize gluten peptides when presented by HLA-DQ2 (DQA1*05/DQB1*02), molecule which immunogenetic studies have identified as conferring susceptibility CD. We previously and characterized three DQ2-restricted epitopes that recognized isolated from CD patients, two immunodominant. Because almost all restricted DQ2, because we detailed...
The purpose of this study was to compare the sampling efficacy rayon swabs and nylon flocked swabs, oropharyngeal nasopharyngeal specimens for detection respiratory viruses in elderly patients. Samples were obtained from patients 60 years age or above who newly admitted Sorlandet Hospital Arendal, Norway. interviewed current symptoms a tract infection. Using comparable sets mucosal samples harvested nasopharynx oropharynx. analysed using real-time polymerase chain reaction (PCR) methods. A...
The tick-borne bacterium Candidatus (Ca.) Neoehrlichia (N.) mikurensis is a cause of "fever unknown origin" because this strict intracellular pathogen escapes detection by routine blood cultures. Case reports suggest that neoehrlichiosis patients may display serological reactivity to Anaplasma (A.) phagocytophilum. Since serology part the diagnostic work-up undetermined fever in European tick-exposed patients, we wanted investigate (1) prevalence A. phagocytophilum seropositivity among (2)...
The knowledge regarding the occurrence and clinical implications of tick-borne infections in immunosuppressed patients living tick-endemic areas is limited.Adult with autoimmune conditions requiring immunosuppressive treatment such as infliximab rituximab were invited to participate study when they attended hospital for and/or control disease. Whole-blood samples analyzed by real-time polymerase chain reaction Borrelia burgdorferi sensu lato, miyamotoi, Anaplasma phagocytophilum, Rickettsia...
The current diagnostic marker of Lyme neuroborreliosis (LNB), the Borrelia burgdorferisensu lato antibody index (AI) in cerebrospinal fluid (CSF), has insufficient sensitivity early phase LNB. We aimed to elucidate value PCR for B. CSF from children with symptoms suggestive LNB and explore genotypes associated children. Children were prospectively included predefined groups a high or low likelihood based on guidelines (LNB symptoms, pleocytosis, antibodies) detection other causative agents....
Introduction Lyme borreliosis (LB) is the most common tick transmitted disease in Europe. The diagnosis of LB today based on patient´s medical history, clinical presentation and laboratory findings. diagnostics are mainly antibody detection, but certain conditions molecular detection by polymerase chain reaction (PCR) may serve as a complement. Aim purpose this study was to evaluate analytical sensitivity, specificity concordance eight different real-time PCR methods at five laboratories...
Tick-borne encephalitis (TBE) is usually diagnosed based on the presence of TBE virus (TBEV)-specific IgM and IgG antibodies in serum. However, induced by vaccination or cross-reactivity to previous flavivirus infections may result false positive TBEV serology. Detection RNA be an alternative diagnostic approach detect viral circumvent difficulties present when using Viral blood commonly detectable only first viremic phase lasting up two weeks, not second neurologic phase, patients contact...
Susceptibility and resistance to type 1 diabetes are associated with MHC class II alleles that carry non-Asp Asp at residue 57 of their beta chain respectively. The effect or non-Aspbeta57 may relate a differential ability distinct molecules bind specific immuno-pathogenic peptides. Recent studies in man mouse have revealed some diabetes-predisposing (i.e. DQ8, DR4Dw15 I-Ag7) preferentially peptides negatively charged anchor P9. It has been suggested this is common feature molecules....
Background A modified microscopy protocol (the LM-method) was used to demonstrate what interpreted as Borrelia spirochetes and later also Babesia sp., in peripheral blood from patients. The method gained much publicity, but not validated prior publication, which became the purpose of this study using appropriate scientific methodology, including a control group. Methods Blood 21 patients previously positive for and/or infection by LM-method 41 healthy controls without known history tick bite...
Celiac disease is an intestinal disorder that develops as a result of interplay between genetic and environmental factors. HLA genes along with non-HLA predispose to the disease. Linkage studies have failed identify chromosomal regions other than region which major effects, indicating existence multiple predisposing modest effects. Association shown CTLA4 or closely located gene one these genes. The primary association in majority celiac patients DQ2 (DQA1*05/DQB1*02) minority DQ8...
We need more knowledge on clinical presentations, time course, biomarkers, and prognosis in European Lyme neuroborreliosis (LNB).
Tests for direct detection of Borrelia burgdorferi sensu lato (Bb) in Lyme neuroborreliosis (LNB) are needed. Detection Bb DNA using PCR is promising, but clinical utility hampered by low diagnostic sensitivity. We aimed to examine whether sensitivity can be improved the use larger cerebrospinal fluid (CSF) volumes and faster handling samples.Patients who underwent CSF examination LNB were included. collected two millilitres analysis, extracted from pellets within 24 h analysed eluate...
Sørlandet sykehus
Tick-borne disease caused by B. miyamotoi (BMD) usually manifest as a febrile illness in humans. Complications include relapsing fever and rare occasions involvement of the central nervous system. Only few cases meningoencephalitis have been described, mostly immunosuppressed patients.A 70-year-old female receiving immunosuppressive rituximab therapy presented with frontal headache, dizziness, nausea, vomiting chills. Clinical laboratory blood analyses were normal. Cerebrospinal fluid (CSF)...