A5090025966- Vector-borne infectious diseases
- Viral Infections and Vectors
- Vector-Borne Animal Diseases
- Dermatological diseases and infestations
- Insect and Pesticide Research
- Mosquito-borne diseases and control
- Yersinia bacterium, plague, ectoparasites research
- Antibiotic Resistance in Bacteria
- Zoonotic diseases and public health
- Bacterial Identification and Susceptibility Testing
- Mycobacterium research and diagnosis
Region Jönköpings län
2016-2024
Linköping University
2017-2023
Ryhov Hospital Jönköping
2015
In north-western Europe, the common tick, Ixodes ricinus, is widely established, its distribution appears to be increasing and spread of tick-borne diseases concern. The project 'Flått i Nord' (Ticks in northern Norway) commenced spring 2009 with intention studying tick's that pathogens Norway. Several methods were used: cloth-dragging, collecting from trapped small mammals, pets. Since 2010, occurrence ticks region Norway was determined directly by cloth-dragging 167 times 109 separate...
Anti-Borrelia antibodies in the cerebrospinal fluid (CSF) are required for definite diagnosis of Lyme neuroborreliosis (LNB). However, children often present with early LNB, and antibody production CSF may not be demonstrated. Recent studies have suggested chemokine CXCL13 to an marker LNB. The aim study was evaluate laboratory pediatric LNB patients association pleocytosis CSF, clinical features, recovery. samples were collected from patients, classified as (n = 44) or possible 22),...
We evaluated the diagnostic performance of two assays, one bead‐based assay and enzyme‐linked immunosorbent (ELISA), for determination CXCL13 levels in cerebrospinal fluid (CSF) from patients with suspected Lyme neuroborreliosis (LNB). Patients investigated LNB were retrospectively included (n = 132): 35 definite LNB, 8 possible CSF pleocytosis but normal antibody index (AI), 6 elevated AI no 83 non‐ patients. samples had been drawn before antibiotic treatment analysed CXCL 13 by Quantikine...
Lyme borreliosis (LB), caused by spirochetes belonging to the Borrelia burgdorferi sensu lato complex, is most common tick-borne infection in Europe. Laboratory diagnosis of LB mainly based on patients' medical history, clinical signs and symptoms combination with detection Borrelia-specific antibodies where indirect enzyme-linked-immunosorbent assay (ELISA) widely used technique. The objective study was evaluate compare diagnostic accuracy (sensitivities specificities) serological tests...
This is the first study to determine density of questing Ixodes ricinus in northern Norway. It was performed at two sites Brønnøy, which has been known for its tick permissive habitats decades and one northernmost with an abundant I. population world. From April November 2011, all stages host-seeking were collected from sites. The overall prevalence nymphs infected Borrelia burgdorferi sensu lato 21% that adult ticks 46%. rates genospecies afzelii, garinii, valaisiana similar findings most...
Introduction Lyme borreliosis (LB) is the most common tick transmitted disease in Europe. The diagnosis of LB today based on patient´s medical history, clinical presentation and laboratory findings. diagnostics are mainly antibody detection, but certain conditions molecular detection by polymerase chain reaction (PCR) may serve as a complement. Aim purpose this study was to evaluate analytical sensitivity, specificity concordance eight different real-time PCR methods at five laboratories...
Lyme neuroborreliosis (LNB), a tick-borne infection caused by spirochetes within the
Migratory birds play a dual role as potential reservoirs of tick-borne pathogens, and dispersers pathogen-containing ticks during their migratory journeys. Ixodes ricinus, prevalent tick species in Northern Western Europe, serves primary vector for Anaplasma phagocytophilum—a bacterium with implications human animal health. There is limited information available regarding A. phagocytophilum birds. Our investigation focused on prevalence collected from southeastern Sweden. The identification...
In Lyme neuroborrelios (LNB), the immune response has been in focus, but association between different cytokines/chemokines and clinical manifestations LNB patients not fully investigated. The aim of this study was to evaluate a large number cytokines chemokines cerebrospinal fluid (CSF) relation diagnosis, presentation recovery children being evaluated for LNB. Pediatric (n = 105) were recruited at seven Swedish pediatric departments during 2010-14. Serum CSF samples drawn on admission,...
Abstract The chemokine CXCL13 is used as complement to serology in the diagnostics of Lyme neuroborreliosis (LNB). We evaluated and compared semi-quantitative, cassette-based ReaScan assay with quantitative recomBead using a collection 209 cerebrospinal fluid samples. categorical agreement between results interpreted negative, grey zone, positive by two methods was 87%. diagnostic sensitivity higher assay, whereas specificity ReaScan. Few manual steps, short turn-around time no batching...
The main tools for clinical diagnostics of Lyme neuroborreliosis (LNB) are based on serology, i.e., detection antibodies in cerebrospinal fluid (CSF). In some cases, PCR may be used as a supplement, e.g., CSF from patients with early LNB. Standardisation the molecular methods and systematic evaluation pre-analytical handling is lacking. To increase analytical sensitivity Borrelia bacteria by targeting 16S rRNA gene, parameters were systematically evaluated samples spiked known amount...
Objectives Lyme serology does not readily discriminate an active borreliosis (LB) from a previous Borrelia infection or exposure. Here, we aimed to investigate large number of immunological protein biomarkers search for pattern typical LB, in contrast patterns found healthy blood donors, proportion whom were previously exposed . Methods Serum samples well-characterised adult patients with ongoing LB and donors included investigated using proximity extension assay (provided by Olink®) which...
Healthcare-associated infections caused by Escherichia coli and antibiotic resistance due to extended-spectrum beta-lactamase (ESBL) production constitute a threat against patient safety. To identify, track, control outbreaks detect emerging virulent clones, typing tools of sufficient discriminatory power that generate reproducible unambiguous data are needed.A probe based real-time PCR method targeting multiple single nucleotide polymorphisms (SNP) was developed. The on the multi locus...