A5083138815- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Viral Infections and Vectors
- Mosquito-borne diseases and control
- Insect symbiosis and bacterial influences
- Insect and Pesticide Research
- Yersinia bacterium, plague, ectoparasites research
- Transgenic Plants and Applications
- Toxin Mechanisms and Immunotoxins
- Bacterial Infections and Vaccines
Amsterdam University Medical Centers
2019-2025
Amsterdam UMC Location University of Amsterdam
2018-2024
University of Amsterdam
2018-2022
National Institute for Public Health and the Environment
2021
Linköping University
2019
Abstract We report 2 human cases of Borrelia miyamotoi disease diagnosed in Sweden, including 1 case meningitis an apparently immunocompetent patient. The diagnoses were confirmed by 3 different independent PCR assays and DNA sequencing from cerebrospinal fluid, supplemented serologic analyses.
Ixodes scapularis is a primary vector of tick-borne pathogens in North America. Repeated exposure to these ticks can induce humoral response tick antigens and acquired resistance. However, identifying contributing this resistance challenging because the vast number I. proteins secreted during feeding. To address this, we developed rapid extracellular antigen monitoring ( Isc REAM), technique detect antibody responses more than 3000 antigens. We validated REAM with immunoglobulin G (IgG) from...
Borrelia miyamotoi is a relapsing fever Borrelia, transmitted by hard (Ixodes) ticks, which are also the main vector for burgdorferi. A widely used test serodiagnosis of Lyme borreliosis an enzyme immunoassay (EIA) based on C6 peptide B. burgdorferi sl VlsE protein. We set out to study reactivity upon infection with in large well-characterized disease (BMD) patient sera and experimental murine infection.We performed silico analyses, comparing C6-peptide immunodominant variable proteins...
Lyme disease, the most common vector-borne illness in North America, is caused by spirochete Borrelia burgdorferi . Infection begins skin following a tick bite and can spread to hearts, joints, nervous system, other organs. Diverse host responses influence level of B infection mice humans. Using systems biology approach, we examined potential molecular interactions between human extracellular secreted proteins A yeast display library expressing 1031 was probed against 36 isolates sensu lato...
This paper describes the protein array as a novel serological test for diagnosis of Borrelia miyamotoi disease (BMD), by reporting methodology, development diagnostic algorithm, and its extensive validation. With rising numbers ticks tick bites, tick-borne diseases, such BMD, urgently deserve further societal medical attention. B. is prevalent in Ixodes across northern hemisphere.
We report the draft whole-genome sequences of two Borrelia miyamotoi strains isolated in The Netherlands. Using next-generation sequencing, we determined complete sequence chromosomes and several plasmids. show a genotype typical European strains, distinct from genomes Asia or United States.
In Europe, the hard tick Ixodes ricinus is considered most important vector of human zoonotic diseases. Human pathogenic agents spread by I. in Sweden include Borrelia burgdorferi sensu lato (s.l.), Anaplasma phagocytophilum, Rickettsia helvetica, recently described Neoehrlichia mikurensis, miyamotoi, tick-borne encephalitis virus (TBEV), and Babesia spp. (Babesia microti, venatorum divergens). Since these pathogens share same vector, co-infections with more than one pathogen may occur thus...
Relapsing fever, caused by diverse Borrelia spirochetes, is prevalent in many parts of the world and causes significant morbidity mortality. To investigate pathoetiology relapsing we performed a high-throughput screen Borrelia-binding host factors using library human extracellular secretory proteins identified CD55 as novel binding partner crocidurae persica, two agents fever Africa Eurasia. present on surface erythrocytes, carries Cromer blood group antigens, protects cells from...
Borrelia miyamotoi is an emerging relapsing fever (RF) species that reported to cause human disease in regions which Lyme borreliosis endemic. We recently showed B. tick isolates are resistant amoxicillin vitro; however, clinical have not been studied. Therefore, our aim was show the antimicrobial susceptibility of obtained A dilution series various antibiotics made modified Kelly-Pettenkofer medium with 10% fetal calf serum. The susceptibilities different clinical, tick, RF Borrelia, and...
Tick-borne disease caused by B. miyamotoi (BMD) usually manifest as a febrile illness in humans. Complications include relapsing fever and rare occasions involvement of the central nervous system. Only few cases meningoencephalitis have been described, mostly immunosuppressed patients.A 70-year-old female receiving immunosuppressive rituximab therapy presented with frontal headache, dizziness, nausea, vomiting chills. Clinical laboratory blood analyses were normal. Cerebrospinal fluid (CSF)...
Abstract The spirochete Borrelia miyamotoi has recently been shown to cause relapsing fever. Like the Lyme disease agent, burgdorferi, B. is transmitted through bite of infected ticks; however, little known about response immune system upon infection. Dendritic cells (DCs) play a central role in early against burgdorferi. We investigated DCs two different strains using vitro and ex vivo models compared this elicited by Our findings show that phagocytosed monocyte-derived DCs, causing...
The impact of infections with tick-borne pathogens (TBPs) other than Borrelia burgdorferi (s.l.) and encephalitis virus (TBEV) on public health in Europe remains unclear. Our goal is to evaluate whether the presence these TBPs ticks can be associated self-reported complaints.We enrolled individuals who were bitten by I. ricinus between 2012 2015 collected their relevant demographic clinical information using a self-administered online questionnaire. A total 4163 sent participants subject...