A5079780121- Viral Infections and Vectors
- Vector-borne infectious diseases
- Vector-Borne Animal Diseases
- Mosquito-borne diseases and control
- Soil and Water Nutrient Dynamics
- Yersinia bacterium, plague, ectoparasites research
- Zoonotic diseases and public health
- Groundwater and Isotope Geochemistry
- Peatlands and Wetlands Ecology
- SARS-CoV-2 and COVID-19 Research
- Insect and Pesticide Research
- Plant Virus Research Studies
- Bacteriophages and microbial interactions
- Fire effects on ecosystems
- Animal Virus Infections Studies
- Soil Carbon and Nitrogen Dynamics
- Education, Psychology, and Social Research
- Viral gastroenteritis research and epidemiology
- Atmospheric chemistry and aerosols
- Hydrology and Watershed Management Studies
- Plant and Fungal Interactions Research
- Smart Materials for Construction
- Forest ecology and management
- Mine drainage and remediation techniques
- Soil erosion and sediment transport
Czech University of Life Sciences Prague
2019-2025
Yale University
2020
Czech Academy of Sciences, Biology Centre
2014-2018
Institute of Parasitology
2014-2018
Veterinary Research Institute
2015-2017
University of South Bohemia in České Budějovice
2014-2015
Czech Academy of Sciences
2015
Czech Geological Survey
1995-2000
The largest outbreak of yellow fever the 21st century in Americas began 2016, with intense circulation southeastern states Brazil, particularly sylvatic environments near densely populated areas including metropolitan region São Paulo city (MRSP) during 2017-2018. Herein, we describe origin and molecular epidemiology virus (YFV) this inferred from 36 full genome sequences taken individuals who died following infection zoonotic YFV. Our analysis revealed that these deaths were due to three...
Tick-borne encephalitis virus (TBEV) is a leading cause of human neuroinfections in Europe and Northeast Asia. There are no antiviral therapies for treating TBEV infection. A series nucleoside analogues was tested the ability to inhibit replication porcine kidney cells neuroblastoma cells. The interactions three with viral polymerase were simulated using advanced computational methods. 7-deaza-2'-C-methyladenosine (7-deaza-2'-CMA), 2'-C-methyladenosine (2'-CMA), 2'-C-methylcytidine (2'-CMC)...
Viral RNA dependent polymerases (vRdPs) are present in all viruses; unfortunately, their sequence similarity is too low for phylogenetic studies. Nevertheless, vRdP protein structures remarkably conserved. In this study, we used the structural of vRdPs to reconstruct evolutionary history. The major strength work unifying and data into a single quantitative analysis, using powerful Bayesian approach. resulting phylogram demonstrates that RNA-dependent DNA (RdDPs) viruses within Retroviridae...
Coronavirus disease 2019 (COVID-19), caused by the severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2), first emerged in Wuhan and rapidly spread worldwide. During course of COVID-19 pandemic, numerous reports highlighted infections wild animals SARS-CoV-2. Nevertheless, further research is required to understand virus potential infect various animal species, which crucial for evaluating its future evolution reemergence The total concentration immunoglobulin G (IgG) represents a...
ABSTRACT Tick-borne encephalitis virus (TBEV) causes a severe and potentially fatal neuroinfection in humans. Despite its high medical relevance, no specific antiviral therapy is currently available. Here we demonstrate that treatment with nucleoside analog, 7-deaza-2′- C -methyladenosine (7-deaza-2′-CMA), substantially improved disease outcomes, increased survival, reduced signs of viral titers the brains mice infected lethal dose TBEV. To investigate mechanism action 7-deaza-2′-CMA, two...
We have identified seven putative guanine quadruplexes (G4) in the RNA genome of tick-borne encephalitis virus (TBEV), a flavivirus causing thousands human infections and numerous deaths every year. The formation G4s was confirmed by biophysical methods on synthetic oligonucleotides derived from predicted TBEV sequences. TBEV-5, located at NS4b/NS5 boundary conserved among all known flaviviruses, tested along with its mutated variants for interactions panel G4 ligands, ability to affect...
Crimean-Congo haemorrhagic fever (CCHF) is the most widely distributed tick-borne viral disease in humans and caused by virus (CCHFV). The has a broader distribution, expanding from western China South Asia to Middle East, southeast Europe, Africa. historical known distribution of CCHFV vector Hyalomma marginatum Europe includes Mediterranean Balkan countries, Ukraine, southern Russia. Further expansion its potential may have occurred out region. This study updated distributional map...
Crimean-Congo haemorrhagic fever orthonairovirus (CCHFV) is a negative-sense, single-stranded RNA virus with segmented genome and the causative agent of severe (CCHF) disease. The transmitted mainly by tick species in Hyalomma genus but other ticks such as representatives genera Dermacentor Rhipicephalus may also be involved life cycle. To improve our understanding CCHFV adaptation to its species, we compared nucleotide composition codon usage patterns among all strains i) which sequences...
The tick species Ixodes uriae, parasitizing seabirds in the Arctic, may transmit many pathogens including various arboviruses, Borrelia spirochetes and Babesia apicomplexans. These pose an important additional stress to seabirds, which are already stressed by environmental changes such as pollutants decreased food availability. Here, we present results of first screening for arboviruses genera Flavivirus, Alphavirus, Orthobunyavirus, Phlebovirus Orbivirus, well apicomplexans from Svalbard...
Lyme disease, the most common vector-borne illness in North America, is caused by spirochete Borrelia burgdorferi . Infection begins skin following a tick bite and can spread to hearts, joints, nervous system, other organs. Diverse host responses influence level of B infection mice humans. Using systems biology approach, we examined potential molecular interactions between human extracellular secreted proteins A yeast display library expressing 1031 was probed against 36 isolates sensu lato...
The SARS-CoV-2 pandemic has heightened interest in the monitoring and surveillance of coronaviruses wildlife. Testing for virus animals can provide valuable insights into viral reservoirs, transmission, pathogenesis. In this study, we present results molecular project focused on Senegalese During project, screened fecal samples wild living Bandia Reserve (ten non-human primates, one giraffe, two white rhinoceros) free-living urban population African four-toed hedgehogs Ngaparou. showed...
As hematophagous parasites, many tick species are important vectors of medical and veterinary disease agents. Proteins found in saliva midgut have been used with some success immunizations animal hosts against feeding ticks, whole has effectively this capacity Ixodes scapularis, the primary vector tickborne pathogens United States. Tick is a complex substance containing hundreds proteins, identification specific protective antigens ongoing. We performed series experiments immunizing guinea...