A5101678267- RNA modifications and cancer
- MicroRNA in disease regulation
- Cancer-related molecular mechanisms research
- Cancer-related gene regulation
- Bone Metabolism and Diseases
- Lipid Membrane Structure and Behavior
- Protein Structure and Dynamics
- Animal Virus Infections Studies
- Protein Kinase Regulation and GTPase Signaling
- Epigenetics and DNA Methylation
- RNA Research and Splicing
- Viral gastroenteritis research and epidemiology
- Electrocatalysts for Energy Conversion
- Cancer, Hypoxia, and Metabolism
- Bone health and treatments
- Catalysis and Oxidation Reactions
- Photoreceptor and optogenetics research
- Neuroscience and Neuropharmacology Research
- Cancer, Lipids, and Metabolism
- Cell death mechanisms and regulation
- Bone health and osteoporosis research
- Spectroscopy Techniques in Biomedical and Chemical Research
- HIV Research and Treatment
- Virus-based gene therapy research
- Circular RNAs in diseases
Frederick National Laboratory for Cancer Research
2020-2024
Norwegian University of Science and Technology
2024
Guangzhou Medical University
2012-2023
Third Affiliated Hospital of Guangzhou Medical University
2014-2023
Leidos Biomedical Research Inc. (United States)
2015-2021
Leidos (United States)
2014-2021
State Key Laboratory of Respiratory Disease
2012-2020
Yan'an University
2020
Second People’s Hospital of Yibin
2018
Sun Yat-sen University
2017
Ferroptosis is a recently recognized form of regulated cell death caused by an iron‐dependent accumulation lipid reactive oxygen species. However, the molecular mechanisms regulating ferroptosis remain obscure. Here, we report that nuclear factor erythroid 2‐related 2 (NRF2) plays central role in protecting hepatocellular carcinoma (HCC) cells against ferroptosis. Upon exposure to ferroptosis‐inducing compounds (e.g., erastin, sorafenib, and buthionine sulfoximine), p62 expression prevented...
N6-methyladenosine (m6A) is the most prevalent modification in eukaryotic RNAs. The biological importance of m6A relies on readers, which control mRNA fate and function. However, it remains unexplored whether additional regulatory subunits readers are involved recognition Here we discover that long noncoding RNA (lncRNA) LINC00266-1 encodes a 71-amino acid peptide. peptide mainly interacts with RNA-binding proteins, including reader IGF2BP1, thus named "RNA-binding peptide" (RBRP). RBRP...
// De Chen 1,4,* , Bian-Li Dang 2,* Jin-zhou Huang Min 2 Di Wu 3 Man-Li Xu Rong Li and Guang-Rong Yan 1,2,4 1 Biomedicine Research Center Department of Surgery, The Third Affiliated Hospital Guangzhou Medicine University, Guangzhou, China Institutes Life Health Engineering, Jinan Cancer Center, Nanfang Hospital, Southern Medical 4 Key Laboratory for Major Obstetric Diseases Guangdong Province Reproduction Genetics Higher Education Institutes, * These authors have contributed equally to...
According to cancer-related microRNA (miRNA) expression microarray research available in public databases, miR-362 is elevated gastric cancer. However, the and biological role of progression remain unclear. levels cancer tissues cell lines were determined using real-time PCR. The roles miR-362, promoting proliferation apoptosis resistance, assessed by different assays, such as colony assay, flow cytometry TUNEL assay. effect on NF-κB activation was investigated luciferase reporter...
Hepatocellular carcinoma (HCC) is one of the most common malignancies and a major cause cancer-related mortality in world. MicroRNAs (miRNAs) are small, noncoding RNAs that play essential roles various stages during cancer progression. The aim current study was to elucidate role miR-1269 pathogenesis HCC.The expression HCC cells tissues were determined by Real-time PCR analysis. Cell viability, colony formation anchorage-independent growth ability assays performed examine cell proliferative...
Hepatocellular carcinoma (HCC) is one of the common malignancies, which highly metastatic and third cause cancer deaths in world. The invasion metastasis cells a multistep complex process mainly initiated by extracellular matrix (ECM) degradation. Aberrant expression microRNA has been investigated HCC shown to play essential roles during progression. In present study, we found that microRNA-324-5p (miR-324-5p) was downregulated both cell lines tissues. Ectopic miR-324-5p led reduction...
Abstract N6-methyladenosine (m 6 A) modification plays important roles in bioprocesses and diseases. AlkB homolog 5 (ALKBH5) is one of two m A demethylases. Here, we reveal that ALKBH5 acetylated at lysine 235 (K235) by acetyltransferase 8 deacetylated histone deacetylase 7. K235 acetylation strengthens the demethylation activity increasing its recognition on mRNA. RNA-binding protein paraspeckle component 1 (PSCP1) a regulatory subunit preferentially interacts with K235-acetylated to...
Abstract The oncogene RAS, extensively studied for decades, presents persistent gaps in understanding, hindering the development of effective therapeutic strategies due to a lack precise details on how RAS initiates MAPK signaling with RAF effector proteins at plasma membrane. Recent advances X-ray crystallography, cryo-EM, and super-resolution fluorescence microscopy offer structural spatial insights, yet molecular mechanisms involving protein-protein protein-lipid interactions RAS-mediated...
We report on a novel technique for sorting and identification of single biological cells food-borne bacteria based laser tweezers Raman spectroscopy (LTRS). With this technique, different physiological states in sample chamber were identified by their spectral signatures then they selectively manipulated into clean collection with optical through microchannel. As an example, we sorted the live dead yeast validated standard staining technique. also demonstrated that existing spoiled foods...
MicroRNAs (miRNAs) are regulatory small non-coding RNAs that can regulate gene expression by binding to elements, such as the promotor 5'UTR, mainly in 3'UTR of mRNA. One miRNA targets many mRNAs, which be regulated miRNAs, leading a complex metabolic network. In our study, we found level miR-590-5p is higher human hepatocellular carcinoma cell line HepG2 than normal L02. Downregulation inhibited proliferation and invasion cells (HCCs). We also showed TGF-beta RII, has been regarded...
Significance HIV-1 full-length RNA must go to specific subcellular compartments carry out its functions as a template for translation of structural and enzymatic proteins the genetic material new virions. mislocalization can affect encoded proteins, causing defects in viral replication. Currently, little is known about how transported cytoplasm. Here, we demonstrate that RNAs use diffusion major mechanism cytoplasmic transport absence group-specific antigen (Gag) even presence sufficient Gag...
The loss of ITGA2 plays an important role in cancer metastasis several solid cancers. However, the molecular mechanism primary cancers remains unclear. In this study, we found that a lower protein level was observed breast compared to adjacent non-cancerous tissues. Interestingly, reduction degree at far more than mRNA level. We further showed translation directly inhibited by miR-373 through binding ITGA2-3'UTR. Silencing detached cell-cell interactions, induced deploymerization stress...
Protein–membrane interactions (PMIs) are ubiquitous in cellular signaling. Initial steps of signal transduction cascades often rely on transient and dynamic with the inner plasma membrane leaflet to populate regulate signaling hotspots. Methods target modulate these could yield attractive tool compounds drug candidates. Here, we demonstrate that conjugation a medium-chain lipid tail covalent K-Ras(G12C) binder MRTX849 at solvent-exposed site enables such direct modulation PMIs. The...
To increase our understanding of the events that lead to HIV-1 genome packaging, we examined dynamics viral RNA and Gag-RNA interactions near plasma membrane by using total internal reflection fluorescence microscopy. We labeled with a photoconvertible Eos protein via an RNA-binding recognizes stem-loop sequences engineered into genome. Near-UV light exposure causes irreversible structural change in alters its emitted from green red. studied photoconverting membrane, monitored population...
Despite recent efforts to understand activities of POU domain class 2 transcription factor 1 (POU2F1), little is known about the roles POU2F1 in hepatocellular carcinoma (HCC) tumorigenesis and its correlation with any clinicopathological feature HCC. In this study, we found that was significantly up-regulated HCC specimens compared adjacent non-cancerous liver specimens. The high protein expression level positively correlated large tumor size, histological grade, metastasis advanced...
S100A16 is a conserved member of the S100 protein family in mammals. Its upregulation was observed many tumors and related to malignant transformation. In this study, we explored independent prognostic value terms overall survival (OS) recurrence-free (RFS) by performing retrospective using data The Cancer Genome Atlas (TCGA)-lung adenocarcinoma (LUAD). Besides, deep sequencing TCGA-LUAD, also association between expression its DNA methylation copy number alterations (CNAs). Results showed...
Osteoprotegerin (OPG), also known as osteoclast inhibitory factor, is a soluble receptor of the tumor necrosis factor superfamily. The protein secreted covalent, disulfide-linked homodimer, which predominant extracellular form (1), and expressed in multiple tissues (1)(2)(3). OPG-mediated pathways might have role osteoporosis (3)(4)(5)(6) because estrogen increases OPG gene expression (4)(5). maintains structure healthy bone inhibits activation differentiation (3)(7). In vascular system,...