A5052914215- Microbial infections and disease research
- Aquaculture disease management and microbiota
- Bacteriophages and microbial interactions
- Herpesvirus Infections and Treatments
- Animal Virus Infections Studies
- Mycobacterium research and diagnosis
- Plant Virus Research Studies
- Viral gastroenteritis research and epidemiology
- Viral Infections and Vectors
- Animal Disease Management and Epidemiology
- Toxoplasma gondii Research Studies
- Milk Quality and Mastitis in Dairy Cows
- Salmonella and Campylobacter epidemiology
- Virology and Viral Diseases
- Blood groups and transfusion
- Vector-Borne Animal Diseases
- Pneumonia and Respiratory Infections
- Plant Pathogenic Bacteria Studies
- Cytomegalovirus and herpesvirus research
- Respiratory viral infections research
- Infectious Diseases and Mycology
- Streptococcal Infections and Treatments
- Virus-based gene therapy research
- Rabies epidemiology and control
- Insect and Pesticide Research
Oaks Hospital
2016-2024
University of Ilorin
2020
Farnham Road Hospital
2015-2017
Animal and Plant Health Agency
2002-2014
Veterinary Medicines Directorate
2001-2012
Imperial College London
2005
Central Veterinary Research Laboratory
1983-1993
Although mycoplasmas possess a very limited genome, little is known about their virulence mechanisms and methods of persistence in the host. Examination wide range mycoplasma species found considerable variation ability to form biofilm. Mycoplasma putrefaciens, M. cottewii, yeatsii, agalactiae bovis produced prolific biofilms. Conversely, highly pathogenic causative agent contagious bovine pleuropneumonia, mycoides subsp. SC, was unable produce Biofilms were be considerably more resistant...
Diagnosis of Mycoplasma infection is normally based on culture and serological tests, which can be time-consuming laborious. A number specific PCRs have been developed but to date there has not a single generic test capable detecting differentiating mycoplasmas species level. This report describes the development new diagnostic PCR 16S rRNA gene with Mycoplasma-specific primers separation product according primary sequence using denaturing gradient gel electrophoresis (DGGE). DGGE enabled...
To obtain improved insights into the complex microbial aetiology of bovine mastitis, this study investigated pathogens involved in cattle mastitis Bosnia and Herzegovina. A total 179 milk samples from cows with clinical (CM) subclinical (SCM), as well eight bulk tank (BTM) 48 dairy farms, were analysed by standard bacteriological mycological methods. Mycoplasma detection identification performed using culture techniques real-time polymerase chain reaction (PCR). 88 (49.2%) positive for known...
The minimum inhibitory concentrations (MICs) and mycoplasmacidal (MMCs) of danofloxacin, florfenicol, oxytetracycline, spectinomycin tilmicosin against 62 recent British field isolates Mycoplasma bovis were determined in vitro by a broth microdilution method. most susceptible to danofloxacin with MIC 90 MMC values 0.5 μg/ml 1.0 μg/ml, respectively. They less florfenicol 16 32 μg/ml. Oxytetracycline had only limited effect the majority tested 50 s 4 respectively 64 more than 128 Nearly 20 per...
Between 1990 and 2000, more than 1600 mycoplasmas the related acholeplasmas were identified from ruminant animals by Mycoplasma Group at Veterinary Laboratories Agency — Weybridge. bovis was most commonly pathogen, mostly pneumonic calves but occasionally cattle with mastitis arthritis. canis first isolated in Britain 1995 number of isolates increased to 18 per cent total 1999. The ELISA for antibodies M detected 1971 positive samples (22 cent) among 8959 serum samples, mainly cattle. Other...
A study was conducted to examine cattle going through abattoirs in Sudan for evidence of contagious bovine pleuropneumonia (CBPP), an important disease sub-Saharan Africa. Approximately 0.6% showed lesions resembling CBPP but the causative pathogen, Mycoplasma mycoides subsp. mycoides, could only be isolated from a small number these suggesting inhibition isolation by irresponsible antibiotic usage before slaughter or other pathogens causing CBPP-like lesions. However, mycoplasmas that were...
This study investigated the presence of contagious bovine pleuropneumonia (CBPP) and caprine pneumonia (CCPP) in ruminants to confirm identify mycoplasma species present. A comprehensive approach was employed, involving serological molecular assays. total 342 cattle 319 goats were tested, with 42 23 showing positive serum samples, along six stored cultures. These samples analyzed using various assays, including IDEXX CBPP CCPP cELISAs, Immunoblot, CapriLAT, BoviLAT, Complement Fixation Test...
Enzyme-linked immunosorbent assays (ELISAs) were developed for the detection of IgG antibody to Salmonella enteritidis in poultry flocks. A lipopolysaccharide (LPS) and heat-extracted (HE) antigen use ELISA evaluated together with rapid slide test (RST), microagglutination (MT) microantiglobulin (MAG) test. In experimentally infected specific pathogen free chickens, good correlation was seen between all tests although, generally, MT MAG detected earlier titres peaked than ELISAs. The LPS HE...
ABSTRACT Denaturing gradient gel electrophoresis (DGGE) of a 16S ribosomal DNA PCR product was used to differentiate 32 mycoplasma species veterinary significance. Twenty-seven (85%) could be differentiated by DGGE. This method enable the rapid identification many for which there is no specific available and are currently identified using culture serological tests.