A5108239938- Microbial infections and disease research
- Aquaculture disease management and microbiota
- Bacteriophages and microbial interactions
- Herpesvirus Infections and Treatments
- Mycobacterium research and diagnosis
- Plant Virus Research Studies
- Animal Disease Management and Epidemiology
- Viral Infections and Vectors
- Vector-borne infectious diseases
- Animal Virus Infections Studies
- Vector-Borne Animal Diseases
- Toxoplasma gondii Research Studies
- Chronic Obstructive Pulmonary Disease (COPD) Research
- Respiratory Support and Mechanisms
- Pharmaceutical Practices and Patient Outcomes
- Heart Failure Treatment and Management
- Emergency and Acute Care Studies
- Effects of Environmental Stressors on Livestock
- Immunodeficiency and Autoimmune Disorders
- Rabies epidemiology and control
- Collaborative Teaching and Inclusion
- thermodynamics and calorimetric analyses
- Bacillus and Francisella bacterial research
- Autoimmune and Inflammatory Disorders Research
- Health Policy Implementation Science
Tripler Army Medical Center
2024
Rhode Island Hospital
2016-2024
Providence College
2019-2024
Janus University
2020
Christopher Newport University
2020
Lahey Medical Center
2020
Lifespan
2018
Animal and Plant Health Agency
2003-2012
KTH Royal Institute of Technology
2010
AlbaNova
2010
Although mycoplasmas possess a very limited genome, little is known about their virulence mechanisms and methods of persistence in the host. Examination wide range mycoplasma species found considerable variation ability to form biofilm. Mycoplasma putrefaciens, M. cottewii, yeatsii, agalactiae bovis produced prolific biofilms. Conversely, highly pathogenic causative agent contagious bovine pleuropneumonia, mycoides subsp. SC, was unable produce Biofilms were be considerably more resistant...
Diagnosis of Mycoplasma infection is normally based on culture and serological tests, which can be time-consuming laborious. A number specific PCRs have been developed but to date there has not a single generic test capable detecting differentiating mycoplasmas species level. This report describes the development new diagnostic PCR 16S rRNA gene with Mycoplasma-specific primers separation product according primary sequence using denaturing gradient gel electrophoresis (DGGE). DGGE enabled...
ABSTRACT Denaturing gradient gel electrophoresis (DGGE) of a 16S ribosomal DNA PCR product was used to differentiate 32 mycoplasma species veterinary significance. Twenty-seven (85%) could be differentiated by DGGE. This method enable the rapid identification many for which there is no specific available and are currently identified using culture serological tests.
ABSTRACT Partial sequences of the RNase P RNA gene ( rnpB ) were obtained from a number hemoplasmas and other Mycoplasma species. Phylogenetic analysis these showed that all present within single clade most closely related to fastidiosum , similar results found with 16S rRNA phylogeny.
Mycoplasma agalactiae is the main cause of contagious agalactia, a serious disease sheep and goats, which has major clinical economic impacts. We have developed multilocus sequence typing (MLST) scheme using sequenced genomes M. strains PG2 5632. An MLST based on genes gltX, metS, gyrB, tufA dnaA was designed in total 3468 bp were analysed for each strain. offers highly discriminatory method capable subdividing 53 into 17 distinct types, largely according to geographical origin. detected...
ABSTRACT Mycoplasma bovis is an important veterinary pathogen causing pneumonia, arthritis, and mastitis in infected cattle. We investigated the genetic diversity of 53 isolates collected United Kingdom between 1996 2002 with pulsed-field gel electrophoresis (PFGE), amplified fragment length polymorphism (AFLP), random polymorphic DNA (RAPD) analysis. In addition, influence variable surface protein (Vsp) profiles on generated molecular typing techniques was studied. Both AFLP RAPD separated...
Mycoplasma agalactiae is the main cause of contagious agalactia, a serious disease sheep and goats, which has major clinical economic impacts. Previous studies M. have shown it to be unusually homogeneous there are currently no available epidemiological techniques enable high degree strain differentiation. We developed variable number tandem repeat (VNTR) analysis using sequenced genome type PG2. The PG2 was found replete with sequences 4 were chosen for further analysis. VNTR 5 located...
Mycoplasmas have been isolated from birds of prey during clinical examinations, but their significance to the health raptors is unclear. We report isolation and characterization four mycoplasmas found in upper respiratory tract sick Eurasian Griffon (Gyps fulvus) that were housed a Sicilian rehabilitation center at Ficuzza, near Palermo Sicily, before reintroduction into wild. These included Mycoplasma gallinarum, an unidentified mycoplasma highly similar glycophilum, two with similarities...
In recent years, the number of reports describing a combination clinical signs in cattle comprising pyrexia, hindlimb and/or udder oedema and prefemoral lymphadenopathy, consistently associated with Mycoplasma wenyonii infection, has increased. However, infection does not always appear to result these typical signs, factors leading their manifestation have still be fully elucidated. This article analyses available evidence suggests some options for diagnosis management.
Mycoplasma species were recovered from 10 cetacean carcasses that stranded around Scotland. phocicerebrale was isolated the lungs of three harbor porpoises (Phocoena phocoena) as well liver one these animals. Novel spp. five additional and kidney another. In addition an isolate closely related to 13CL obtained a Sowerby's beaked whale (Mesoplodon bidens). The role in disease cetaceans, their host specificity, diversity, any relation strandings are unknown.
We describe an allele specific PCR based approach for the rapid detection of two bovine Mycoplasma species associated with respiratory disease. Specific and universal oligonucleotides were used in combination to detect presence single nucleotide polymorphisms within 16S ribosomal DNA sequence. Presence rDNA is indicated by production a control fragment, whilst positive samples generate alternative smaller product over same region. This technique provides reliable sensitive method which,...