A5019822761- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Mycotoxins in Agriculture and Food
- Viral Infections and Immunology Research
- Plant Disease Resistance and Genetics
- Plant Pathogens and Fungal Diseases
- Viral gastroenteritis research and epidemiology
- T-cell and Retrovirus Studies
- Animal Virus Infections Studies
- Higher Education Teaching and Evaluation
- Viral Infectious Diseases and Gene Expression in Insects
- Microbial infections and disease research
- Veterinary medicine and infectious diseases
- Carcinogens and Genotoxicity Assessment
- Wheat and Barley Genetics and Pathology
- Meat and Animal Product Quality
- vaccines and immunoinformatics approaches
- Pediatric health and respiratory diseases
- Poxvirus research and outbreaks
- Education and Art Integration
- Agriculture, Plant Science, Crop Management
- Listeria monocytogenes in Food Safety
- Virology and Viral Diseases
- Insect Utilization and Effects
- Hepatitis B Virus Studies
Centre de Recerca en Sanitat Animal
2014-2025
Universitat Autònoma de Barcelona
2012-2025
Institut National de la Recherche Agronomique
2006-2008
African swine fever is a highly contagious viral disease of mandatory declaration to the World Organization for Animal Health (OIE). The lack available vaccines makes its control difficult; thus, virus (ASFV) represents major threat industry. Inactivated do not confer solid protection against ASFV. Conversely, live attenuated viruses (LAV), either naturally isolated or obtained by genetic manipulation, have demonstrated reliable homologous ASFV strains, although little no has been...
The lack of available vaccines against African swine fever virus (ASFV) means that the evaluation new immunization strategies is required. Here we show fusion extracellular domain ASFV Hemagglutinin (sHA) to p54 and p30, two immunodominant structural viral antigens, exponentially improved both humoral cellular responses induced in pigs after DNA immunization. However, with resulting plasmid (pCMV-sHAPQ) did not confer protection lethal challenge virulent E75 ASFV-strain. Due fact CD8(+)...
African swine fever is one of the most devastating pig diseases, against which there no vaccine available. Recent work from our laboratory has demonstrated protective potential DNA vaccines encoding three viral antigens (p54, p30, and hemagglutinin extracellular domain) fused to ubiquitin. Partial protection was afforded in absence detectable antibodies prior virus challenge, survival correlated with presence a large number hemagglutinin-specific CD8(+) T cells blood. Aiming demonstrate...
African swine fever virus (ASFV) is the causal agent of fever, a hemorrhagic and often lethal porcine disease causing enormous economical losses in affected countries. Endemic for decades most sub-Saharan countries Sardinia, risk ASFV-endemicity Europe has increased since its last introduction into 2007. Live attenuated viruses have been demonstrated to induce very efficient protective immune responses, albeit time protection was circumscribed homologous ASFV challenges. However, their use...
African swine fever virus (ASFV) is causing a worldwide pandemic affecting the porcine industry and leading to important global economic consequences. The causes highly lethal hemorrhagic disease in wild boars domestic pigs. Lack of effective vaccines hampers control spread, thus increasing pressure on scientific community for urgent solutions. However, knowledge immune components associated with protection very limited. Here we characterized vitro recall response induced by cells from pigs...
The DNA that encodes the 5.8S gene of ribosomal RNA and two intergenic spacers ITS1 ITS2 proposed type strains Aspergillus niger aggregate (A. tubingensis) have been sequenced. By comparison sequences we found both species could be differentiated by RsaI digestion PCR products mentioned regions. This method a useful tool in identification A. aggregate, especially studies involve large number isolates.
Deoxynivalenol (DON), a mycotoxin produced by Fusarium spp., is frequent contaminant of cereals. Because their rich cereal diet, pigs could be exposed to this mycotoxin. Pigs are among the animal species showing greatest sensitivity DON. Effects intermediate high levels DON on well known and include feed refusal, decreased intake, alteration immune response. low DON, which commonly detected in contaminated feed, remain unknown. The aim study was investigate effect diet naturally with...
African swine fever (ASF) has become the major threat for global industry. Furthermore, epidemiological situation of virus (ASFV) in some endemic regions Sub-Saharan Africa is worse than ever, with multiple strains and genotypes currently circulating a given area. Despite recent advances on ASF vaccine development, there are no commercial vaccines yet, most promising prototypes available today have been specifically designed to fight genotype II Europe, Asia, Oceania. Previous results from...
African swine fever (ASF) is today′s number one threat for the global industry. Neither commercial vaccine nor treatment available against ASF and, thus far, only live attenuated viruses (LAV) have provided robust protection lethal virus (ASFV) challenge infections. Identification of ASFV proteins inducing protective immune responses major challenges to develop safer and efficient subunit vaccines. Immunopeptidomic studies recently performed in our laboratory allowed identifying antigens...
African swine fever virus (ASFV) is known to be very stable within a protein-rich environment and indirect transmission can mediated via oral uptake of different materials. However, experimental studies in pigs have shown that infection by ASFV the route difficult establish. Currently, there lack using strict inoculations with doses ASFV. Therefore, we aimed determine dose European genotype II required establish route. In this study, 24 were divided into four groups six. Three fed low,...
The development of subunit vaccines against African swine fever (ASF) is mainly hindered by the lack knowledge regarding specific ASF virus (ASFV) antigens involved in protection. As a good example, identity ASFV-specific CD8+ T-cell determinants remains largely unknown, despite their protective role being established long time ago. Aiming to identify them, we implemented IFNγ ELISpot as readout assay, using effector cells peripheral blood mononuclear (PBMCs) from pigs surviving experimental...
Abstract African swine fever virus (ASFV) is the causative agent of a devastating hemorrhagic disease (ASF) that affects both domestic pigs and wild boars. Conversely, ASFV circulates in subclinical manner pigs, including warthogs, natural reservoir for ASFV. Together with genetic differences, other factors might be involved differential susceptibility to ASF observed among Eurasian suids ( Sus scrofa ) warthogs Phacochoerus africanus ). Preliminary evidence obtained our laboratory others,...
African swine fever (ASF) has become the major threat to global industry. Lack of available commercial vaccines complicates implementation control strategies. So far, only live attenuated ASF viruses (ASFV) have demonstrated solid protection efficacy at experimental level. The molecular techniques allowed generation a collection deletion mutants lacking ASFV-specific virulence factors, some them with promising potential as vaccine candidates against pandemic genotype II ASFV strain currently...
Knowledge about African swine fever virus (ASFV) transmission and its survival in the environment is mandatory to develop rational control strategies combat this serious disease pigs. In study, risk that environmental contamination poses for infection of naïve pigs was investigated. Naïve were introduced as sentinels into contaminated pens kept at ambient temperature (about 18–22˚C) either on same day or up 3 days after ASFV-infected removed. Three experiments carried out which four six per...