A5065973762- Animal Disease Management and Epidemiology
- Viral Infections and Immunology Research
- Vector-Borne Animal Diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Viral gastroenteritis research and epidemiology
- Irrigation Practices and Water Management
- Endodontics and Root Canal Treatments
- Advanced Surface Polishing Techniques
- Medical research and treatments
- Advanced Machining and Optimization Techniques
- Health and Medical Education
- Advanced Sensor Technologies Research
- Influenza Virus Research Studies
- SARS-CoV-2 and COVID-19 Research
- Helminth infection and control
- Human Rights and Immigration
- Herpesvirus Infections and Treatments
- Hydraulic flow and structures
- Ethics and bioethics in healthcare
- Ocular Surface and Contact Lens
- Immigration and Intercultural Education
- T-cell and Retrovirus Studies
- Advanced Fiber Optic Sensors
- vaccines and immunoinformatics approaches
- Aging, Health, and Disability
Universidad Autónoma de Madrid
2008-2023
Centro de Biología Molecular Severo Ochoa
1999-2023
National University of Tucumán
2020
Consejo Superior de Investigaciones Científicas
2000-2017
African swine fever is a highly contagious viral disease of mandatory declaration to the World Organization for Animal Health (OIE). The lack available vaccines makes its control difficult; thus, virus (ASFV) represents major threat industry. Inactivated do not confer solid protection against ASFV. Conversely, live attenuated viruses (LAV), either naturally isolated or obtained by genetic manipulation, have demonstrated reliable homologous ASFV strains, although little no has been...
Abstract Growing African swine fever virus (ASFV) isolates obtained mainly from the field, but also engineered in laboratory, is a critical step for diagnosis, titration, or infection studies. This unit describes set of methods and protocols to produce titrate any ASFV strain cell cultures. The procedures include (1) basic techniques prepare virus‐sensitive target cells; (2) strategies growth, concentration, purification stocks; (3) semi‐quantitative (end dilution) quantitative (plaque)...
The African swine fever virus (ASFV) protein pE248R, encoded by the gene E248R, is a late structural component of particle. contains intramolecular disulfide bonds and has been previously identified as substrate ASFV-encoded redox system. Its amino acid sequence putative myristoylation site hydrophobic transmembrane region near its carboxy terminus. We show here that pE248R myristoylated during infection associates with membrane fraction in infected cells, behaving an integral protein....
Foot-and-mouth disease virus (FMDV) causes a highly contagious of cloven-hoofed animals whose control relies on efficient vaccination. We have reported that dendrimer peptide B2T, with two copies FMDV B-cell epitope VP1 (136–154) linked through maleimide units to T-cell 3A (21–35)], elicits potent B- and specific responses confers solid protection in pigs type-O challenge after doses peptide. Herein we now show B2T evokes protective immune administration single dose either 2 or 0.5 mg High...
Antiviral compounds are needed in the control of many animal and human diseases.We analysed effect antitumoural drug lauryl gallate on infectivity African swine fever virus among other DNA (herpes simplex vaccinia) RNA (influenza, porcine transmissible gastroenteritis Sindbis) viruses, paying attention to its viability corresponding host cells.Viral production was strongly inhibited different cell lines at non-toxic concentrations (1-10 microM), reducing titres 3->5 log units depending...
Synthetic dendrimer peptides are a promising strategy to develop new FMD vaccines. A peptide, termed B2T-3A, which harbors two copies of the major FMDV antigenic B-cell site [VP1 (140-158)], covalently linked heterotypic T-cell from non-structural protein 3A [3A (21-35)], has been shown protect pigs against viral challenge. Interestingly, modular design this peptide allows modifications aimed at improving its immunogenicity, such as replacement epitope moiety. Here, we report that B2T-3D,...
Dendrimeric peptide constructs based on a lysine core that comprises both B- and T-cell epitopes of foot-and-mouth disease virus (FMDV) have proven successful strategy for the development FMD vaccines. Specifically, B 2 T dendrimers displaying two copies major type O FMDV antigenic B-cell epitope located capsid [VP1 (140–158)], covalently linked to heterotypic from either non-structural protein 3A [3A (21–35)] or 3D [3D (56–70)], named T-3A T-3D, respectively, elicit high levels neutralizing...
Foot-and-mouth disease virus (FMDV) causes a widely extended contagious of livestock. We have previously reported that synthetic dendrimeric peptide, termed B2 T(mal), consisting two copies B-cell epitope [VP1(140-158)] linked through maleimide groups to T-cell [3A(21-35)] FMDV, elicits potent B- and T-cell-specific responses confers solid protection in pigs type O FMDV challenge. Longer duration the protective response possibility inducing after single dose are important requirements for an...
A broadly protective and biosafe vaccine against foot-and-mouth disease virus (FMDV) remains an unmet need in the animal health sector. We have previously reported solid protection serotype O FMDV afforded by dendrimeric peptide structures harboring virus-specific B- T-cell epitopes, also shown such type of multivalent presentations to be advantageous over simple B-T-epitope linear juxtaposition. Chemically, our platforms are modular constructions readily made from specified epitope...
Dendrimer peptides are promising vaccine candidates against the foot-and-mouth disease virus (FMDV). Several B-cell epitope (B2T) dendrimers, harboring a major FMDV antigenic site in VP1 protein, covalently linked to heterotypic T-cell epitopes from 3A and/or 3D proteins, and elicited consistent levels of neutralizing antibodies IFN-γ-producing cells pigs. To address contribution highly polymorphic nature porcine MHC (SLA, swine leukocyte antigen) on immunogenicity B2T low-resolution (Lr)...
Antiviral compounds targeting cellular metabolism are part of the therapeutic arsenal to control spread virus infection, either as sole treatment or in combination with direct-acting antivirals (DAA) vaccines. Here, we describe effect two them, lauryl gallate (LG) and valproic acid (VPA) both exhibiting a wide antiviral spectrum, against infection by coronaviruses such HCoV-229E, HCoV-OC43, SARS-CoV-2. A consistent 2 4-log-decrease yields was observed presence each antiviral, an average IC50...
Antiviral compounds targeting cellular metabolism instead of virus components have become an interesting issue for preventing and controlling the spread infection, either as sole treatment or a complement vaccination. Some these are involved in control lipid and/or membrane rearrangements. Here, we describe effect three cell-targeting antivirals: lauryl gallate (LG), valproic acid (VPA) cerulenin (CRL) multiplication viruses causing important porcine diseases. The results confirm antiviral...
Abstract Elucidation of the molecular basis stability foot-and-mouth disease virus (FMDV) particles is relevant to understand key aspects cycle. Residue N17D in VP1, located at capsid inner surface, modulates resistance FMDV virion dissociation and inactivation acidic pH. Here we have studied whether virion-stabilizing effect amino acid substitution VP1 may be mediated by alteration electrostatic charge this position and/or presence viral RNA. Substitutions that either introduced a positive...
An approach based on a dendrimer display of B- and T-cell epitopes relevant for antibody induction has been shown to be effective as foot-and-mouth disease (FMD) vaccine. B2T dendrimers combining two copies the major FMD virus (FMDV) type O B-cell epitope (capsid proteinVP1 (140–158)) covalently linked heterotypic from non-structural protein 3A (21–35), henceforth B2T-3A, previously elicit high neutralizing (nAb) titers IFN-γ-producing cells in both mice pigs. Here, we provide evidence that...