A5069314917- Animal Disease Management and Epidemiology
- Viral Infections and Immunology Research
- Vector-Borne Animal Diseases
- interferon and immune responses
- Viral Infections and Vectors
- Viral Infectious Diseases and Gene Expression in Insects
- Virology and Viral Diseases
- RNA Research and Splicing
- Animal Virus Infections Studies
- Immune Response and Inflammation
- RNA and protein synthesis mechanisms
- Mosquito-borne diseases and control
- RNA regulation and disease
- Autoimmune and Inflammatory Disorders Research
- Media Studies and Communication
- Viral Infections and Outbreaks Research
- Diabetes and associated disorders
Centro de Biología Molecular Severo Ochoa
2014-2025
Universidad Autónoma de Madrid
2014-2025
Universidad Libre de Colombia
2025
Consejo Superior de Investigaciones Científicas
2012-2023
Centre de Recerca en Sanitat Animal
2011
Centro de Investigación en Sanidad Animal
2008-2009
Centro Regional de Selección y Reproducción Animal
2008-2009
The untranslated regions (UTRs) of the foot-and-mouth disease virus (FMDV) genome contain multiple functional elements. In 5' UTR, internal ribosome entry site (IRES) element governs cap-independent translation initiation, whereas S region is presumably involved in RNA replication. 3' composed two stem-loops and a poly(A) tract, required for viral infectivity stimulates IRES activity. Here, it was found that end established distinct strand-specific, long-range RNA-RNA interactions, one with...
Abstract Propagation of viruses requires interaction with host factors in infected cells and repression innate immune responses triggered by the viral sensors. Cytosolic DNA sensing pathway cyclic GMP-AMP synthase (cGAS) stimulator interferon genes (STING) is a major component antiviral response to viruses, also known play relevant role infection RNA including foot-and-mouth disease virus (FMDV). Here, we provide supporting evidence cGAS degradation swine during FMDV show that two virally...
Post-chikungunya viral arthritis may persist for months to years after infection and is characterized by relapsing remitting symptoms. This study investigates the relationship between autoantibodies chikungunya severity, providing insights into pathogenesis. We assessed measures in a cohort of serologically confirmed cases from Colombia 2019 2021 (n = 144). measured disease flare intensity, pain, disability, then plasma antibody levels rheumatoid factor IgM, anti-cyclic citrullinated peptide...
Abstract The nonstructural protein 1 (NSP1) of SARS-CoV-2 blocks the messenger RNA (mRNA) entry channel 40S ribosomal subunit, causing inhibition translation initiation and subsequent degradation host mRNAs. However, target mRNA specificity how viral mRNAs escape NSP1-mediated have not been clarified to date. Here we found that NSP1 acts as a translational switch capable blocking or enhancing depending on preinitiation complex, 43S-PIC, is recruited mRNA, whereas mostly depends codon usage...
The RNA helicase LGP2 (Laboratory of Genetics and Physiology 2) is a non-signaling member the retinoic acid-inducible gene-I (RIG-I)-like receptors (RLRs), whose pivotal role on innate immune responses against viruses being increasingly uncovered. known to work in synergy with melanoma differentiation-associated gene 5 (MDA5) promote antiviral response induced by picornavirus infection. Here, we describe activity foot-and-mouth disease virus (FMDV) Leader protease (Lpro) targeting for...
We constructed foot-and-mouth disease virus (FMDV) mutants bearing independent deletions of the two stem-loop structures predicted in 3' noncoding region viral RNA, SL1 and SL2, respectively. Deletion SL2 was lethal for infectivity cultured cells, while deletion resulted viruses with slower growth kinetics downregulated replication associated impaired negative-strand RNA synthesis. With aim exploring potential an RNA-based vaccine against using attenuated genomes, full-length chimeric...
The induction of type I interferons (alpha/beta interferon [IFN-α/β]) in response to viral infection is a crucial step leading the antiviral state host. Viruses produce double-stranded RNA (dsDNA) during their replication cycle that sensed as nonself by host cells through different receptors. A signaling cascade then activated block and spread. Foot-and-mouth disease virus (FMDV) picornavirus highly sensitive IFN, it causes one world's most important animal diseases. In this study, we showed...
The innate immune system is the first line of defense against viral infections. Exploiting responses for antiviral, therapeutic and vaccine adjuvation strategies being extensively explored. We have previously described, ability small in vitro RNA transcripts, mimicking sequence structure different domains non-coding regions foot-and-mouth disease virus (FMDV) genome (ncRNAs), to trigger a potent rapid response. These synthetic non-infectious molecules proved broad-range antiviral activity...
ABSTRACT Foot-and-mouth disease virus (FMDV) nonstructural protein 3A plays important roles in replication, virulence, and host range. In other picornaviruses, homodimerization of has been shown to be relevant for its biological activity. this work, FMDV was evidenced by an situ fluorescent ligation assay. A molecular model the protein, derived from nuclear magnetic resonance (NMR) structure poliovirus predicted a hydrophobic interface spanning residues 25 44 as main determinant...
Abstract The RIG-I-like receptor (RLR) melanoma differentiation-associated gene 5 (MDA5) plays a key role in triggering innate antiviral response during infection by RNA viruses. MDA5 activation leads to transcription induction of type-I interferon (IFN) and proinflammatory cytokines. has also been associated with autoimmune autoinflammatory diseases dysfunctional immune the absence infection. Here, we show how foot-and-mouth disease virus (FMDV) counteracts specific effect exerted targeting...
West Nile virus (WNV) is a neurovirulent single stranded RNA mosquito-borne flavivirus, whose main natural hosts are birds, but it also infects humans and horses. Nowadays, no human vaccine commercially available clinical treatment only supportive. Recently, has been shown that transcripts, mimicking structural domains in the non-coding regions (NCRs) of foot-and mouth disease (FMDV) induce potent IFN response antiviral activity transfected cultured cells, reduced mice susceptibility to...
Foot-and-mouth disease virus (FMDV) causes a widely extended contagious of livestock. We have previously reported that synthetic dendrimeric peptide, termed B2 T(mal), consisting two copies B-cell epitope [VP1(140-158)] linked through maleimide groups to T-cell [3A(21-35)] FMDV, elicits potent B- and T-cell-specific responses confers solid protection in pigs type O FMDV challenge. Longer duration the protective response possibility inducing after single dose are important requirements for an...
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is the causative agent of a potentially severe disease, disease 2019 (COVID-19), an ongoing pandemic with limited therapeutic options. Here, we assessed anti-coronavirus activity synthetic RNAs mimicking specific domains in non-coding regions foot-and-mouth virus (FMDV) genome (ncRNAs). These molecules are known to exert broad-spectrum antiviral cell culture, mice and pigs effectively triggering host innate immune response. The...
Engineered RNAs carrying substitutions in the integrin receptor-binding Arg-Gly-Asp (RGD) region of foot-and-mouth disease virus (FMDV) were constructed (aa 141-147 VP1 capsid protein) and their infectivity was assayed cultured cells suckling mice. The effect these changes studied proteins two FMDVs, C-S8c1, which enters through integrins, 213hs(-), a derivative highly adapted to cell culture whose ability infect using glycosaminoglycan heparan sulfate (HS) as receptor, acquired by multiple...
A broadly protective and biosafe vaccine against foot-and-mouth disease virus (FMDV) remains an unmet need in the animal health sector. We have previously reported solid protection serotype O FMDV afforded by dendrimeric peptide structures harboring virus-specific B- T-cell epitopes, also shown such type of multivalent presentations to be advantageous over simple B-T-epitope linear juxtaposition. Chemically, our platforms are modular constructions readily made from specified epitope...
The role of cellular Rab GTPases that govern traffic between different endosome populations was analysed on foot-and-mouth disease virus (FMDV) infection. Changes viral receptor specificity did not alter Rab5 requirement for However, a correlation uncoating pH and infection observed. A mutant FMDV with less acidic threshold sensitive to inhibition Rab5, whereas another more requirements Rab5. On the contrary, opposed correlations dependence function were observed upon expression...