A5013049913- HIV Research and Treatment
- HIV/AIDS drug development and treatment
- Ruminant Nutrition and Digestive Physiology
- Genetic and phenotypic traits in livestock
- HIV/AIDS Research and Interventions
- Hepatitis C virus research
- Gut microbiota and health
- Hepatitis B Virus Studies
- HIV-related health complications and treatments
- SARS-CoV-2 detection and testing
- Advanced biosensing and bioanalysis techniques
- Genomics and Phylogenetic Studies
- Bacteriophages and microbial interactions
- Reproductive Physiology in Livestock
- Cytomegalovirus and herpesvirus research
- Liver Disease Diagnosis and Treatment
- DNA and Nucleic Acid Chemistry
- Evolution and Genetic Dynamics
- SARS-CoV-2 and COVID-19 Research
- Agriculture Sustainability and Environmental Impact
- Vitamin D Research Studies
- Anaerobic Digestion and Biogas Production
- HER2/EGFR in Cancer Research
- Animal Disease Management and Epidemiology
- Environmental DNA in Biodiversity Studies
Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria
2019-2025
Consejo Superior de Investigaciones Científicas
1997-2022
Escuela Nacional de Sanidad
2019
Instituto de Salud Carlos III
2004-2019
Centro Nacional de Microbiologia
2004-2018
Universidad Autónoma de Madrid
1997-2008
Centro de Biología Molecular Severo Ochoa
2001-2008
Centro Regional de Selección y Reproducción Animal
2008
Centro de Investigación en Sanidad Animal
2008
The advent of metagenomics in animal breeding poses the challenge statistically modelling relationship between microbiome, host genetics and relevant complex traits. A set structural equation models (SEMs) a recursive type within Markov chain Monte Carlo (MCMC) framework was proposed here to jointly analyse host-metagenome-phenotype relationship. non-recursive bivariate model as benchmark compare model. relative abundance rumen microbes (RA), methane concentration (CH4 ) used case study....
Abstract Background Mitigating the effects of global warming has become main challenge for humanity in recent decades. Livestock farming contributes to greenhouse gas emissions, with an important output methane from enteric fermentation processes, mostly ruminants. Because ruminal microbiota is directly involved digestive processes and biosynthesis, understanding ecological relationships between rumen microorganisms their active metabolic pathways essential reducing emissions. This study...
The catalytic efficiency of incorporation deoxyribonucleotides by wild-type human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) was around 100-fold higher than for dideoxyribonucleotides, in Mg2+-catalyzed reactions, and more 10,000-fold nucleotides having a 2′-hydroxyl group Mg2+- Mn2+-catalyzed reactions. Mutant RTs with nonconservative substitutions affecting Tyr-115 (Y115V, Y115A, Y115G) showed dramatic reduction their ability to discriminate against ribonucleotides...
Genetic material from rumen microorganisms can be found within the oral cavity, and hence there is potential in using microbiome as a proxy of ruminal microbiome. Feed intake (FI) influences composition microbiota might directly influence dairy cattle. Ruminal content samples (RS) 29 cows were collected at beginning study also 42 d later (RS0 RS42, respectively). Additionally, 18 through buccal swabbing (OS42) randomly selected cows. Samples used to characterize compare taxonomy...
Oxford Nanopore sequencing is a long-read technology that does not rely on polymerase to generate sequence data. Sequencing library preparation methods used in the addition of motor protein bound an adapter sequence, which added either using ligation-based (ligation kit), or transposase-based (rapid kit). However, these have enzymatic steps may be susceptible motif bias, including underrepresentation adenine-thymine (AT) sequences due ligation and biases from transposases. This study aimed...
A compensatory mutation (M230I) in the primer grip of human immunodeficiency virus type 1 (HIV-1) reverse transcriptase (RT) restores replication capacity having a Y115W their RT coding region. The substitution impairs DNA polymerase activity and produces an enzyme with lower fidelity synthesis. Gel-based assays double mutant Y115W/M230I revealed that M230I increased accuracy Y115W. showed wild-type misinsertion performed DNA/DNA templates. However, when present alone, conferred reduced as...
Exosome-derived miR-21 was independently associated with CD4 T cell decline in HIV-1-infected elite controllers (OR 0.369, 95% CI 0.137-0.994, p = 0.049). Also, a negative correlation between expression and MCP-1 level found (r -0.649, 0.020), while no soluble biomarkers or cellular immune activation found.
Methane is a potent greenhouse gas produced during the ruminal fermentation and associated with loss of feed energy. Therefore, efforts to reduce methane emissions have been ongoing in last decades. production highly influenced by factors such as microbiome host genetics. Previous studies proposed use long-term emissions, composition moderately heritable trait genetic improvement accumulates over time. Lactation stage another important factor that might influence but potential associations...
Resistance-associated variants have been related to treatment failure of hepatitis C virus (HCV) therapy with direct-acting antiviral drugs. The aim our study was analyze the prevalence clinically relevant resistance-associated within NS3 in patients infected HCV genotype 1a (GT1a) Spain.We performed a cross-sectional on 2568 from 115 hospitals throughout Spain (2014-2015). viral protease gene amplified by nested polymerase chain reaction and sequenced Sanger sequencing using an ABI PRISM...
Rumen microorganisms carry antimicrobial resistance genes which pose a threaten to animals and humans in One Health context. In order tackle the emergence of it is vital understand how they appear, their relationship with host, behave as whole ruminal ecosystem or spread environment humans. We sequenced samples from 416 Holstein dairy cows 14 Spanish farms using nanopore technology, uncover presence potential effect on human, animal environmental health.We found 998 (ARGs) cow rumen studied...
Engineered RNAs carrying substitutions in the integrin receptor-binding Arg-Gly-Asp (RGD) region of foot-and-mouth disease virus (FMDV) were constructed (aa 141-147 VP1 capsid protein) and their infectivity was assayed cultured cells suckling mice. The effect these changes studied proteins two FMDVs, C-S8c1, which enters through integrins, 213hs(-), a derivative highly adapted to cell culture whose ability infect using glycosaminoglycan heparan sulfate (HS) as receptor, acquired by multiple...
Objective: To analyze the association between patatin-like phospholipase domain-containing 3 gene (PNPLA3) rs738409 polymorphism and severity of liver disease in HIV/hepatitis C virus-coinfected patients. Methods: We performed a cross-sectional study 215 patients who underwent biopsy. PNPLA3 was genotyped using GoldenGate assay. The outcome variables were as follows: advanced fibrosis (F ≥3 FIB-4 ≥3.25), rapid progression (FPR ≥0.10 units/year), severe activity grade (A≥3), steatosis (fatty...
Objective We investigated the association of genetic polymorphisms in chemokine and receptor genes with poor immunological recovery HIV patients starting combined antiretroviral therapy (cART) low CD4 T-cell counts. Methods A case-control study was conducted 412 HIV-infected cART count <200 cells/μL successful viral control for two years. increase below 200 after years on used to define INR (immunological non-responder) patients. Polymorphisms CXCL12, CCL5 CCR2 were genotyped using...
<title>Abstract</title> Background Nanopore sequencing is a long-read technology that does not rely on polymerase to generate sequence data. Sequencing library preparation methods used in nanopore the addition of motor protein bound an adapter sequence, which added either using ligation-based (ligation kit), or transposase-based (rapid kit). However, these have enzymatic steps may be susceptible motif bias, including underrepresentation adenine-thymine (AT) sequences due ligation and biases...
Abstract Relevant resistance-associated substitutions (RASs) to elbasvir, the new HCV NS5A inhibitor, may limit its efficacy and lead virological failure in HCV-GT1a-infected patients. There are few data outside clinical trials evaluating their prevalence impact of elbasvir/grazoprevir. A multicenter cross-sectional study 617 individuals attended 84 Spanish hospitals from 17 Autonomous Communities two cities was performed. population sequencing used identify RASs elbasvir mutational pattern...
Objective: Vitamin D has been linked to the immune response modulation and integrity of intestinal mucosal barrier. Therefore, vitamin might be involved in bacterial translocation related HIV infection. Our major aim was analyze association between plasma levels 25-hydroxy-vitamin [25(OH)D] 16S ribosomal DNA (bactDNA) 120 HIV/hepatitis c virus (HCV) coinfected patients. Design: Cross-sectional study. Methods: Plasma 25(OH)D were quantified by enzyme immunoassay. The status defined as...