Introduction of African swine fever virus (ASFV) into pig herds can occur via virus-contaminated feed or other objects. Knowledge about ASFV survival in different matrices and under conditions is required to understand indirect transmission. Maintenance infectivity for extended periods outside pigs. Current assays detecting have inherent disadvantages. Cell culture-based are labor-intensive sensitive contaminants while methods using qPCR detect DNA with high sensitivity specificity, but this...

African swine fever virus (ASFV) is known to be very stable within a protein-rich environment and indirect transmission can mediated via oral uptake of different materials. However, experimental studies in pigs have shown that infection by ASFV the route difficult establish. Currently, there lack using strict inoculations with doses ASFV. Therefore, we aimed determine dose European genotype II required establish route. In this study, 24 were divided into four groups six. Three fed low,...

Bat species worldwide are receiving increased attention for the discovery of emerging viruses, cross-species transmission, and zoonoses, as well characterizing virus infections specific to bats. In a previous study, we investigated presence coronaviruses in faecal samples from bats at different locations Denmark, made phylogenies based on short, partial ORF1b sequences. this selected containing bat three were analysed, using non-targeted approach next-generation sequencing. From resulting...

Bat populations harbour a multitude of viruses; some these are pathogenic or potentially in other animals humans. Therefore, it is important to monitor the and characterize viruses. In this study, presence coronaviruses (CoVs) different species Danish bats was investigated using active surveillance at geographical locations Denmark. Faecal samples were screened for CoVs pan-CoV real-time RT-PCR assays. The amplicons, obtained from five bats, sequenced. Phylogenetic analysis revealed...

Insects represent a sustainable source of food and feed. In order to increase the sustainability insect production, new feed substrates should be explored including waste materials that contain animal by-products. However, use products does come with potential risks for transmission pathogens. Experimental exposure studies insects selected pathogens can provide knowledge some these future quantitative risk assessments. this study, we have developed virus bioexposure assays examine uptake...

Insect production offers a sustainable source of nutrients for livestock. This comes with risk transmission pathogens from the insects into livestock sector, including viruses causing serious diseases, such as African swine fever virus (ASFV), classical and foot-and-mouth disease virus. ASFV is known to survive long time within animal meat byproducts. Therefore, we conducted experimental exposure studies using larvae two key insect species produced food feed, mealworm; Tenebrio molitor,...

African swine fever virus (ASFV) has become widespread in Europe, Asia and elsewhere, thereby causing extensive economic losses. The viral genome includes nearly 200 genes, but their expression within infected pigs not been well characterized previously. In this study, four were with a genotype II strain (ASFV POL/2015/Podlaskie); blood samples collected before inoculation at both 3 6 days later. During period, range of clinical signs infection became apparent the pigs. From blood,...

It is important to be able detect and differentiate between distinct porcine enteric coronaviruses that can cause similar diseases. However, the existence of naturally occurring recombinant such as swine coronavirus (SeCoV) give misleading results with currently used diagnostic methods. Therefore, we have developed validated three duplex real-time quantitative RT-PCR assays for simultaneous detection of, differentiation between, epidemic diarrhea virus (PEDV) SeCoV. Transmissible...

African swine fever virus (ASFV) is known to be very stable within a protein-rich environment and indirect transmission can mediated via oral uptake of different materials. However, experimental studies in pigs have shown that infection by ASFV the route difficult establish. Currently, there lack using strict inoculations with doses ASFV. Therefore, we aimed determine dose European genotype II required establish route. In this study, 24 were divided into four groups six. Three fed low,...

Insect larvae production offers the potential for large-scale synthesis of high quality protein that can be used as feed or food. However, currently, there are limitations on source substrates insect to use. One concern is survival animal pathogens within if their contaminated. In this study, a very stable virus, porcine parvovirus (PPV), mealworm (Tenebrio molitor) and black soldier fly (BSF) (Hermetia illucens) has been analyzed after oral ingestion virus. The PPV DNA genome could readily...

Porcine epidemic diarrhea virus (PEDV), belonging to the genus Alphacoronavirus, can cause serious disease in pigs of all ages, especially suckling pigs. Differences virulence have been observed between various strains this virus. In study, four were inoculated with PEDV from Germany (intestine/intestinal content collected 2016) and Italy material 2016). The re-inoculated same on multiple occasions create a more robust infection enhance antibody responses. clinical signs pathological changes...

It is important to be able detect and differentiate between distinct porcine enteric coronaviruses that can cause similar diseases. However, the existence of naturally occurring recombinant such as swine coronavirus (SeCoV) give misleading results with currently used diagnostic methods. Therefore, we have developed validated three duplex real-time quantitative RT-PCR assays for simultaneous detection of, differentiation between, epidemic diarrhea virus (PEDV) SeCoV. Transmissible...

Insect larvae production offers the potential for large-scale synthesis of high-quality protein that can be used as feed or food. However, currently, there are limitations on source substrates insect to use. One concern is survival animal pathogens within if their contaminated. In this study, a very stable virus, porcine parvovirus (PPV), mealworm (Tenebrio molitor) and black soldier fly (BSF) (Hermetia illucens) has been analyzed after oral ingestion virus. PPV genomic DNA could readily...

Introduction of African swine fever virus (ASFV) into pig herds can occur via virus-contaminated feed or other objects. Knowledge about ASFV survival in different matrices and under conditions is required to understand indirect transmission. Maintenance infectivity occurs for extended periods outside pigs but depends on the contaminated material environmental factors. Current assays detecting have inherent disadvantages. Cell culture-based are labor-intensive sensitive contaminants. Methods,...