A5037617983- Animal Disease Management and Epidemiology
- Vector-Borne Animal Diseases
- Animal Virus Infections Studies
- Viral Infections and Immunology Research
- Viral gastroenteritis research and epidemiology
- SARS-CoV-2 and COVID-19 Research
- Virus-based gene therapy research
- Animal Behavior and Welfare Studies
- T-cell and Retrovirus Studies
- Viral Infectious Diseases and Gene Expression in Insects
- SARS-CoV-2 detection and testing
- T-cell and B-cell Immunology
- Effects of Environmental Stressors on Livestock
- Viral Infections and Vectors
- COVID-19 epidemiological studies
- Insect Utilization and Effects
- COVID-19 Clinical Research Studies
- Milk Quality and Mastitis in Dairy Cows
- Modeling and Simulation Systems
- Virology and Viral Diseases
- Sensor Technology and Measurement Systems
- Viral Infections and Outbreaks Research
- Agriculture and Farm Safety
- Wireless Body Area Networks
- Plant and Fungal Interactions Research
Statens Serum Institut
2020-2025
Technical University of Denmark
2012-2024
University of Copenhagen
2020
Danish Ministry of Food, Agriculture and Fisheries
2004
Abstract Severe acute respiratory syndrome coronavirus 2 has caused a pandemic in humans. Farmed mink (Neovison vison) are also susceptible. In Denmark, this virus spread rapidly among farmed mink, resulting some disease. Full-length genome sequencing revealed novel variants mink. These subsequently appeared within the local human community.
In June-November 2020, SARS-CoV-2-infected mink were detected in 290 of 1,147 Danish farms. North Denmark Region, 30% (324/1,092) people found connected to farms tested SARS-CoV-2-PCR-positive and approximately 27% (95% confidence interval (CI): 25-30) SARS-CoV-2-strains from humans the community mink-associated. Measures proved insufficient mitigate spread. On 4 November, government ordered culling all mink. Farmed constitute a potential virus reservoir challenging pandemic control.
SARS-CoV-2 infection is the cause of COVID-19 in humans. In April 2020, farmed mink (Neovision vision) occurred Netherlands. The first outbreaks Denmark were detected June 2020 three farms. A steep increase number infected farms from September and onwards. Here, we describe prevalence data collected 215 to characterize spread impact disease one third farms, no clinical signs observed. with signs, decreased feed intake, increased mortality respiratory symptoms most frequently observed, during...
Within Eastern Europe, African swine fever virus (ASFV) has unexpectedly spread to farms with high biosecurity. In an attempt explain this process, pigs were allowed ingest flies that had fed on ASFV-spiked blood, which a realistic titre for infected pig. Some of the became virus. Thus, ingestion blood-sucking flies, having ASFV-infected wild boar before entering stables, represents potential route disease transmission.
Since the introduction of African swine fever virus (ASFV) into Baltic states and Poland in 2014, disease has continued to spread within these regions. In 2017, further west first cases were reported Czech Republic Romania, wild boar domestic pigs, respectively. To control spread, knowledge different modes transmission, including indirect transmission via a contaminated environment, is crucial. Up until now, such an mode not been demonstrated. this study, environment with excretions from...
Foot-and-mouth disease (FMD) is a viral of livestock with substantial impact on agricultural production and subsistence farming global scale. Control FMD impeded by the existence prolonged asymptomatic carrier phase during which infected cattle shed low quantities infectious virus in oropharyngeal fluid (OPF) for months to years after infection. The epidemiological significance (FMDV) carriers unresolved. However, FMDV state has international trade animal products. current investigation...
In addition to humans, severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) can transmit animals that include hamsters, cats, dogs, mink, ferrets, tigers, lions, cynomolgus macaques, rhesus and treeshrew. Among these, mink are particularly susceptible. Indeed, 10 countries in Europe North America reported SARS-CoV-2 infection among on fur farms. Denmark, spread rapidly farms spilled-over back into acquiring mutations/deletions with unknown consequences for virulence antigenicity....
African swine fever virus (ASFV) is known to be very stable within a protein-rich environment and indirect transmission can mediated via oral uptake of different materials. However, experimental studies in pigs have shown that infection by ASFV the route difficult establish. Currently, there lack using strict inoculations with doses ASFV. Therefore, we aimed determine dose European genotype II required establish route. In this study, 24 were divided into four groups six. Three fed low,...
Mink, on a farm with about 15,000 animals, became infected SARS-CoV-2. Over 75% of tested animals were positive for SARS-CoV-2 RNA in throat swabs and 100% seropositive. The virus responsible had deletion nucleotides encoding residues H69 V70 within the spike protein gene as well A22920T mutation, resulting Y453F substitution this protein, seen previously mink. mink recovered after free-testing 300 (a level giving 93% confidence detecting 1% prevalence), remained During further follow-up...
Foot-and-mouth disease (FMD) remains one of the most economically important infectious diseases production animals globally. Vaccination can successfully control this disease, however, current vaccines are imperfect. They made using chemically inactivated FMD virus (FMDV) that is produced in large-scale mammalian cell culture under high containment conditions. Here, we have expressed FMDV capsid protein precursor (P1-2A) strain O1 Manisa alone or with 3C protease (3Cpro) a "single cycle"...
Insects represent a sustainable source of food and feed. In order to increase the sustainability insect production, new feed substrates should be explored including waste materials that contain animal by-products. However, use products does come with potential risks for transmission pathogens. Experimental exposure studies insects selected pathogens can provide knowledge some these future quantitative risk assessments. this study, we have developed virus bioexposure assays examine uptake...
Knowledge about African swine fever virus (ASFV) transmission and its survival in the environment is mandatory to develop rational control strategies combat this serious disease pigs. In study, risk that environmental contamination poses for infection of naïve pigs was investigated. Naïve were introduced as sentinels into contaminated pens kept at ambient temperature (about 18–22˚C) either on same day or up 3 days after ASFV-infected removed. Three experiments carried out which four six per...
African swine fever virus (ASFV) is the causative agent of fever, an economically important disease pigs, often with a high case fatality rate. ASFV has demonstrated low genetic diversity among isolates collected within Eurasia. To explore influence viral variants on clinical outcomes and infection dynamics in pigs experimentally infected ASFV, we have designed deep sequencing strategy. The variant analysis revealed unique SNPs at <10% frequency several as well some that were found more...
Outbreaks of porcine epidemic diarrhoea (PED) were reported across Europe during the 1980s and 1990s, but only sporadic outbreaks occurred in recent years. PED virus (PEDV) spread for first time into USA 2013 has caused severe economic losses. Retrospectively, it was found that two different strains PEDV have been introduced United States, both are closely related to circulating China where a new wave disease from 2010 onwards. Since autumn 2014, Europe. In this study, weaned piglets...
Insect production offers a sustainable source of nutrients for livestock. This comes with risk transmission pathogens from the insects into livestock sector, including viruses causing serious diseases, such as African swine fever virus (ASFV), classical and foot-and-mouth disease virus. ASFV is known to survive long time within animal meat byproducts. Therefore, we conducted experimental exposure studies using larvae two key insect species produced food feed, mealworm; Tenebrio molitor,...
Several studies have demonstrated a consistent increase in the CD8+ T-cell subset of pigs following infection with porcine reproductive and respiratory virus (PRRSV). Consequently, it has been suggested that T-cells may play an important role protection against this infection. In order to test hypothesis, we examined five 5-week-old pigs, which had depleted for by treatment anti-CD8 mAb injections, starting 2 days before inoculation PRRSV. Virus-inoculated sham-inoculated age-matched served...
Abstract Background The applicability of an electronic monitoring system using microchip transponders for measurement body temperatures was tested in 6-week-old conventional Danish weaners infected with classical swine fever virus (CSFV). Subcutaneous tissue obtained by the implantable were compared rectal temperatures, recorded a digital thermometer. Methods In preliminary study, inserted subcutaneously at 6 different positions 5 pigs. positioned ear base provided best correlation to...
African swine fever virus (ASFV) has become widespread in Europe, Asia and elsewhere, thereby causing extensive economic losses. The viral genome includes nearly 200 genes, but their expression within infected pigs not been well characterized previously. In this study, four were with a genotype II strain (ASFV POL/2015/Podlaskie); blood samples collected before inoculation at both 3 6 days later. During period, range of clinical signs infection became apparent the pigs. From blood,...