A5026027888- HIV, Drug Use, Sexual Risk
- Animal Disease Management and Epidemiology
- CRISPR and Genetic Engineering
- HIV Research and Treatment
- Vector-Borne Animal Diseases
- HIV/AIDS Research and Interventions
- Biosensors and Analytical Detection
- Diabetes, Cardiovascular Risks, and Lipoproteins
- Viral gastroenteritis research and epidemiology
- Identification and Quantification in Food
- Virus-based gene therapy research
- Viral Infectious Diseases and Gene Expression in Insects
- Mosquito-borne diseases and control
- Advanced biosensing and bioanalysis techniques
- Environmental DNA in Biodiversity Studies
- T-cell and Retrovirus Studies
- Cytomegalovirus and herpesvirus research
- Animal Genetics and Reproduction
- Viral Infections and Immunology Research
International Livestock Research Institute
2021-2023
Masinde Muliro University of Science and Technology
2018-2022
The Rapid Visual CRISPR (RAVI-CRISPR) assay employs Cas12a and Cas13a enzymes for precise gene detection in a sample. However, RAVI-CRISPR is limited single-tube multiplex applications due to the lack of specific single-strand (ss) DNA-fluorescently quenched (ssDNA-FQ) RNA-fluorescently (ssRNA-FQ) reporter cleavage mechanisms. We report development sensitive dual-gene diagnostic system. To optimize application field testing, we designed portable fluorescence imaging that could distinguish...
Infection of pigs with the African swine fever virus (ASFV) leads to a devastating hemorrhagic disease high mortality up 100%. In this study, CD2v gene deletion was introduced genotype IX from East Africa, ASFV-Kenya-IX-1033 (ASFV-Kenya-IX-1033-∆CD2v), investigate whether led reduced virulence in domestic and see if inoculation LA-ASFV could induce protective immunity against parental challenge. All inoculated ASFV-Kenya-IX-1033-ΔCD2v survived but presented fever, appetite lethargy. ASFV...
The growing demand for and supply of meat products has led to a proportional increase in cases adulteration. Adulterated poses serious economic health consequences globally. Current laboratory methods species identification require specialized equipment with limited field applications. This study developed an inexpensive, point-of-care Loop-Mediated Isothermal Amplification (LAMP)-CRISPR/Cas12a colorimetric assay detect using Texas Red-labelled single-strand (ssDNA) reporter. As low as 1.0...
African swine fever virus (ASFV) is the causative agent of (ASF), resulting in up to 100% mortality pigs. Although endemic most sub-Saharan countries, where all known ASFV genotypes have been reported, disease has caused pandemics significant economic impact Eurasia, and no vaccines or therapeutics are available date. In endeavors develop live-attenuated against ASF, deletions several ~170 genes shown contrasting results depending on genotype investigated ASFV. Here, we report vivo outcome a...
We describe the characterization of an African swine fever genotype IX virus (ASFV-Kenya-IX-1033), which was isolated from a domestic pig in western Kenya during reported outbreak. This includes efficiency replication and vivo virulence, together with genome stability following passage blood macrophages wild boar lung cell line (WSL). The ASFV-Kenya-IX-1033 stock retained its ability to replicate primary virulence vivo, more than 20 passages WSL. At whole level, few single-nucleotide...
Illicit substance use and HIV infection cause haematological derangements. Anaemia characterized by a reduction in the quality quantity erythrocytes is most common disorder both HIV-positive persons illicit users.To describe anaemia burden, types, its association with injectable users Mombasa, Kenya.This descriptive case-control study evaluated red cell indices morphology 494 adults. The primary outcome was anaemia. of injection determined using chi-square test.The participants included 275...
Abstract In this study, we describe an African swine fever genotype IX virus (ASFV-Kenya-1033-IX), which was isolated from a domestic pig in Western Kenya during reported outbreak, including efficiency of replication, vivo virulence, and genome stability pulmonary alveolar macrophages (PAM) wild boar cell line (WSL). The ASFV-Kenya-1033-IX stock, underwent multiple passages WSL (more than 20), retained its ability to replicate primary it also the virulence . At genomic level, only few single...
Abstract The impact of injection and non-injection substance use in HIV infections is an area great public importance especially with respect to hematologic immune profiles. Evaluations status are critical for better disease classification clinical management positive users. However, not much information known about the derangements infected This study, therefore, aimed at determining laboratory markers Hematologic profiles were evaluated on venous blood specimens obtained from users, ISU...