A5014460683- Agriculture, Plant Science, Crop Management
- Animal Disease Management and Epidemiology
- Herpesvirus Infections and Treatments
- Vector-Borne Animal Diseases
- Garlic and Onion Studies
- Yeasts and Rust Fungi Studies
- Plant Pathogens and Resistance
- CRISPR and Genetic Engineering
- Plant Pathogens and Fungal Diseases
- Viral Infectious Diseases and Gene Expression in Insects
- Plant pathogens and resistance mechanisms
- Plant Disease Resistance and Genetics
- Plant nutrient uptake and metabolism
- Wheat and Barley Genetics and Pathology
- Horticultural and Viticultural Research
- Environmental Science and Technology
- Cytomegalovirus and herpesvirus research
- Viral Infections and Immunology Research
- Virus-based gene therapy research
- Fungal Biology and Applications
- Fermentation and Sensory Analysis
- Allelopathy and phytotoxic interactions
- Plant Virus Research Studies
- Plant Physiology and Cultivation Studies
- Insect Pest Control Strategies
Friedrich-Loeffler-Institut
2009-2025
University of Zurich
2016
Universitätsklinik für Kinder und Jugendpsychiatrie
2016
Innsbruck Medical University
2016
Institute for the Sociology of Law and Criminology
2012
Institute of Molecular Biology
2000-2009
Institute of Molecular Biology
2000
RWTH Aachen University
1982-1984
Friedrich-Alexander-Universität Erlangen-Nürnberg
1975
Institut für Pflanzenkultur (Germany)
1935-1973
The complete genome of Marek's disease virus serotype 1 (MDV-1) strain 584Ap80C was cloned in Escherichia coli as a bacterial artificial chromosome (BAC). BAC vector sequences were introduced into the U(S)2 locus MDV-1 by homologous recombination. Viral DNA containing used to transform DH10B, and several colonies harboring an F plasmid (MDV-1 BACs) identified. from various BACs transfected chicken embryo fibroblasts, 3 days after transfection, infectious obtained. Growth recovered...
Abstract African swine fever is a devastating viral disease of domestic and wild pigs against which no vaccine or therapy available. Therefore, we applied the CRISPR (clustered regularly interspaced short palindromic repeats) – Cas9 nuclease system to target double-stranded DNA genome virus (ASFV). To this end, permissive boar lung (WSL) cell line was modified by stable transfection with plasmid encoding guide RNA targeting codons 71 78 phosphoprotein p30 gene (CP204L) ASFV. Due targeted...
Understanding African swine fever virus (ASFV) transmission is essential for strategies to minimize spread during an outbreak. ASFV can survive extended time periods in animal products, carcasses, and the environment. While genome was found environments around infected farms, data on survival soil are scarce. We investigated different matrices spiked with ASFV-positive blood from wild boar see if remain infectious beneath carcasses. As expected, detection possible over entire sampling...
Gap junctions (GJs) play a pivotal role in intercellular communication between eukaryotic cells, including transfer of biomolecules that contribute to the innate and adaptive immune response. However, if how viruses affect gap junction (GJIC) remains largely unexplored. Here, we describe alphaherpesvirus pseudorabies virus (PRV) triggers ERK1/2-mediated phosphorylation main component connexin 43 (Cx43) closure GJIC, which depends on viral protein pUL46. Consequently, UL46null PRV mutant is...
We have cloned and sequenced the Kpnl L M restriction fragments of infectious laryngotracheitis virus (ILTV, gallid herpesvirus 1) DNA, which are localized adjacently at right end unique long region genome. Within 6930 bp DNA sequence six complete open reading frames (ORFs) were identified. The predicted amino acid sequences four them exhibit significant homologies to UL5 (helicase), UL4, UL3 UL2 (uracil-DNA glycosylase) genes, conserved in similar arrangement all alpha-herpesvirus genomes...
African swine fever virus (ASFV) is the causative agent of (ASF), resulting in up to 100% mortality pigs. Although endemic most sub-Saharan countries, where all known ASFV genotypes have been reported, disease has caused pandemics significant economic impact Eurasia, and no vaccines or therapeutics are available date. In endeavors develop live-attenuated against ASF, deletions several ~170 genes shown contrasting results depending on genotype investigated ASFV. Here, we report vivo outcome a...
The DNA sequence of the infectious laryngotracheitis virus (ILTV) UL50, UL51 and UL52 gene homologues was determined. Although deduced UL50 protein lacks first five conserved domains corresponding proteins mammalian alphaherpesviruses, ILTV product also shown to possess dUTPase activity. generation UL50-negative mutants facilitated by recombination plasmids encoding green fluorescent (GFP), expression constructs predicted transactivator (αTIF, ICP4) were successfully used increase...
Infectious laryngotracheitis is an important respiratory disease of chickens that caused by alphaherpesvirus [infectious virus (ILTV); Gallid herpesvirus 1]. As envelope glycoproteins are main targets the humoral host immune response, they particular interest for development vaccines, as well diagnostic tools. The conserved, N-glycosylated protein gC has been identified a major surface antigen ILTV. To study function gC, we now isolated gC-deleted ILTV recombinant rescuant after...
African swine fever virus (ASFV) is the etiological agent of a contagious and fatal disease domestic pigs that has significant economic consequences for global industry. Due to lack effective treatment vaccines against fever, there an urgent need leverage cutting-edge technologies cost-effective approaches generating purifying recombinant fast-track development live-attenuated ASFV vaccines. Here, we describe use CRISPR/Cas9 gene editing cloning system produce ASFVs. Combining these...
We describe the characterization of an African swine fever genotype IX virus (ASFV-Kenya-IX-1033), which was isolated from a domestic pig in western Kenya during reported outbreak. This includes efficiency replication and vivo virulence, together with genome stability following passage blood macrophages wild boar lung cell line (WSL). The ASFV-Kenya-IX-1033 stock retained its ability to replicate primary virulence vivo, more than 20 passages WSL. At whole level, few single-nucleotide...
The membrane protein encoded by the US9 gene of alphaherpesviruses plays an important role during virion assembly and transport in neurons. Here, we demonstrate that herpes simplex virus type 1 (HSV-1) strain KOS, due to base substitutions, predicted TATA-box is mutated, a premature stop present at codon 58 US9, which contains 91 codons other HSV-1 strains. mutation also removes native adjacent US8A gene, leading extension coding region from 160 191 codons. Northern blot analyses revealed...
African swine fever (ASF) is a lethal disease of domestic pigs and wild boar, against which no vaccines are available to date. The large dsDNA genome virus (ASFV) contains up 167 ORFs predicted encode proteins. functions antigenic properties many these proteins still unknown, impedes vaccine development. Based on the results mass spectrometry-based proteome analyses ASFV-infected cells, two highly abundant but previously uncharacterized viral proteins, p285L pK145R, were investigated in...