A5021115647- Herpesvirus Infections and Treatments
- Virology and Viral Diseases
- Mosquito-borne diseases and control
- Cytomegalovirus and herpesvirus research
- Immunotherapy and Immune Responses
- Viral Infections and Vectors
- T-cell and B-cell Immunology
- Toxoplasma gondii Research Studies
- Vector-borne infectious diseases
- interferon and immune responses
- Vector-Borne Animal Diseases
- Connexins and lens biology
Ghent University
2017-2025
Gap junctions (GJs) play a pivotal role in intercellular communication between eukaryotic cells, including transfer of biomolecules that contribute to the innate and adaptive immune response. However, if how viruses affect gap junction (GJIC) remains largely unexplored. Here, we describe alphaherpesvirus pseudorabies virus (PRV) triggers ERK1/2-mediated phosphorylation main component connexin 43 (Cx43) closure GJIC, which depends on viral protein pUL46. Consequently, UL46null PRV mutant is...
Plasmacytoid dendritic cells (pDC) play a central role in the antiviral immune response, both innate response and shaping adaptive mainly because of their ability to produce massive amounts type I interferon (TI-IFN). Here, we report that infected with live attenuated Bartha vaccine strain porcine alphaherpesvirus pseudorabies virus (PRV) trigger dramatically increased TI-IFN by primary pDC compared wild-type PRV strains (Becker Kaplan). Since is one relatively few examples highly successful...
Pseudorabies virus (PRV) is a porcine alphaherpesvirus and the causative agent of Aujeszky's disease. Successful eradication campaigns against PRV have largely relied on use potent vaccines. The live attenuated Bartha strain, which was produced by serial passaging in cell culture, represents one hallmark Despite robust protection elicited vaccination, very little known about immunogenicity strain. Previously, we showed that Bartha-infected epithelial cells trigger plasmacytoid dendritic...
Pseudorabies virus (PRV) is a member of the alphaherpesvirus subfamily and causative agent Aujeszky's disease in pigs. Driven by large economic losses associated with PRV infection, several vaccines vaccine programs have been developed. To this day, attenuated Bartha strain, generated serial passaging, represents golden standard for vaccination. However, proteomic comparison virion to wild-type (WT) virions lacking. Here, we present comprehensive mass spectrometry-based proteome strain three...
Plasmacytoid dendritic cells (pDC) are important innate immune during the onset of viral infections as they specialized in production massive amounts antiviral type I interferon (IFN). Alphaherpesviruses such herpes simplex virus (HSV) or pseudorabies (PRV) double stranded DNA viruses and potent stimulators pDC. Detailed information on how PRV activates porcine pDC is lacking. Using primary pDC, we report here that virions, so-called heavy (H-)particles, trigger IFNα by whereas light (L-)...
Herpes simplex virus (HSV) in humans and pseudorabies (PRV) pigs are both alphaherpesviruses. Plasmacytoid dendritic cells (pDCs) make part of the peripheral blood mononuclear (PBMCs) specialized producing large amounts antiviral type I interferon (IFN-I). IFN-I production by PBMCs response to HSV-1 PRV can be virtually exclusively attributed pDCs. Recently, we discovered that infected with gEnull trigger increased IFNalpha porcine PBMCs/pDCs compared wild-type (WT) PRV. This correlates...