A5084656029- Microbial infections and disease research
- Aquaculture disease management and microbiota
- Bacteriophages and microbial interactions
- Animal Virus Infections Studies
- Herpesvirus Infections and Treatments
- Antibiotic Resistance in Bacteria
- SARS-CoV-2 and COVID-19 Research
- Animal Disease Management and Epidemiology
- Genomics and Phylogenetic Studies
- Antimicrobial Resistance in Staphylococcus
- Viral gastroenteritis research and epidemiology
- Cytomegalovirus and herpesvirus research
- Clostridium difficile and Clostridium perfringens research
- Animal health and immunology
- Trypanosoma species research and implications
- Probiotics and Fermented Foods
- Vector-borne infectious diseases
- Antibiotics Pharmacokinetics and Efficacy
- Virus-based gene therapy research
- Gut microbiota and health
- SARS-CoV-2 detection and testing
- Respiratory viral infections research
- Mycobacterium research and diagnosis
- COVID-19 Clinical Research Studies
- Veterinary medicine and infectious diseases
Ghent University
2020-2025
Ghent University Hospital
2021-2024
Instituut voor Landbouw en Visserijonderzoek
2022
Ghent University Global Campus
2018-2020
Vrije Universiteit Brussel
2018-2020
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is not always confined to the system, as it impacts people on a broad clinical spectrum from asymptomatic severe systemic manifestations resulting in death. Further, accumulation of intra-host single nucleotide variants during prolonged SARS-CoV-2 may lead emergence concern (VOCs). Still, information virus infectivity and evolution across organs sparse. We report detailed virological analysis thirteen postmortem disease...
Abstract Varicellovirus equidalpha 1, formerly known as Equid alphaherpesvirus 1 (EHV-1), is highly prevalent and can lead to various problems, such respiratory abortion, neonatal foal death, neurological disorders. The latter equine herpes myeloencephalopathy (EHM). Cases of EHM have significantly increased since the beginning twenty-first century. genomic sequences five isolates associated with fatal outbreak in Valencia, Spain, 2021 were analyzed documented. genome replication kinetics...
Abstract Background Acute Hepatopancreatic Necrosis Disease (AHPND) causes significant mortality in shrimp aquaculture. The infection is primarily instigated by Vibrio parahaemolyticus ( Vp ) strains carrying a plasmid encoding the binary toxin PirAB. Yet, comprehension of supplementary virulence factors associated with this relatively recent disease remains limited. Furthermore, same holds for gastroenteritis humans caused other genotypes. Additionally, given prevalent use antibiotics to...
Implementation of Third-Generation Sequencing approaches for Whole Genome (WGS) all-in-one diagnostics in human and veterinary medicine, requires the rapid accurate generation consensus genomes. Over last years, Oxford Nanopore Technologies (ONT) released various new devices (e.g. Flongle R9.4.1 flow cell) bioinformatics tools 2019-released Bonito basecaller), allowing cheap user-friendly cost-efficient introduction NGS workflows. While single read, overall accuracies, completeness genome...
Mycoplasma bovis causes many health and welfare problems in cattle. Due to the absence of clear insights regarding transmission dynamics lack a registered vaccine Europe, control an outbreak depends mainly on antimicrobial therapy. Unfortunately, susceptibility testing (AST) is usually not performed, because it time-consuming no standard protocol or clinical breakpoints are available. Fast identification genetic markers associated with acquired resistance may at least partly resolve former...
Abstract Background Respiratory infections are the main indication for antimicrobial use in calves. Optimal treatment duration currently is unknown, but shorter would likely decrease selection resistance. Hypothesis/Objectives Determine differences cure rate and healing time between animals treated with florfenicol oxytetracycline a natural outbreak of respiratory disease using reaeration observed on thoracic ultrasound examination as criterion. Animals Commercial farm housing 130, 3 to 9...
Porcine Parvovirus Type 1 (PPV1) contributes to important losses in the swine industry worldwide. During a PPV1 infection, embryos and fetuses are targeted, resulting stillbirth, mummification, embryonic death, infertility (SMEDI syndrome). Even though vaccination is common gilts sows, strains mainly belonging 27a-like group have been spreading Europe since early 2000s, SMEDI problems requiring in-depth studies into molecular epidemiology efficacy of commercial vaccines. Here, we show that...
To date, no comprehensive diagnostics for the study of polymicrobial infections that are associated with porcine respiratory disease have been offered. This precludes proper understanding entire landscape, thereby hampering effective preventive and therapeutic actions.
Enterotoxigenic
Introduction The intensification of aquaculture to meet the growing demand for aquatic animal protein by a global population approaching 10 billion 2050 has raised concerns about increased risk disease outbreaks in farmed species. These diseases account over 50% economic losses commercial aquaculture, largely due reliance on ineffective and harmful therapeutic options like antibiotics, which contribute multidrug resistance pose serious health concerns. However, non-antibiotic alternatives...
Quick thoracic ultrasonography (qTUS) is increasingly used as an on-farm method to diagnose clinical and subclinical pneumonia in dairy calves. The primary objective of this prospective cohort study was describe dynamics lung consolidation a purchase-dependent production system for male calves relation antimicrobial therapy respiratory diagnostics. In addition, we studied the association cured uncured with average daily gain (ADG) cold carcass weight (CCW). third determine effects arriving...
Rapid identification of Mycoplasma bovis infections in cattle is a key factor to guide antimicrobial therapy and biosecurity measures. Recently, Nanopore sequencing became an affordable diagnostic tool for both clinically relevant viruses bacteria, but the accuracy M. undocumented.
Dromedary camels are an important source of food and income in many countries. However, it has been largely overlooked that they can also transmit antibiotic-resistant bacteria. The aim this study was to identify the Staphylococcaceae bacteria composition nasal flora dromedary evaluate presence methicillin-resistant Mammaliicoccus (MRM) Staphylococcus (MRS) Algeria. Nasal swabs were collected from 46 seven farms located two different regions Algeria (M'sila Ouargla). We used non-selective...
Five equine herpesvirus 1 (EHV-1) genome sequences with links to an EHV-1 outbreak neurological disorders after a horse gathering in Valencia, Spain, February 2021, were determined. All strains showed the closest relationships from Belgium and United Kingdom, indicating common source of infection.
To face the continuous emergence of SARS-CoV-2 variants, broadly protective therapeutic antibodies are highly needed. We here focused on fusion peptide (FP) region viral spike antigen since it is conserved among alpha- and betacoronaviruses. First, we found that coronavirus cross-reactive commonly formed during infection, being omnipresent in sera from COVID-19 patients, ~50% pre-pandemic human (rich against endemic coronaviruses), even feline coronavirus-infected cats. Pepscan analyses...
Abstract M. bovis is one of the leading causes respiratory disease and antimicrobial use in cattle. The pathogen widespread different cattle industries worldwide, but highest prevalence found veal industry. Knowledge on strain distribution over dairy, beef crucial for design effective control prevention programs, currently undocumented. Therefore, present study evaluated molecular epidemiology genetic relatedness isolates obtained from Belgian beef, dairy farms, how these relate to strains...
Infections with Brachyspira hyodysenteriae, the etiological agent of swine dysentery, result in major economic losses pig industry worldwide. Even though microbial differentiation various species can be obtained via PCR, no quick diagnostics for antimicrobial susceptibility testing are place, which is mainly due to time-consuming (4 7 days) anaerobic growth requirements these organisms. Veterinarians often rely on a clinical diagnosis initiating treatment. These treatments not always...
Abstract Although the role of iron in bacterial infections has been well described for Staphylococcus ( S .) aureus , acquisition (bovine-associated) non- staphylococci and mammaliicocci (NASM) remains insufficiently mapped. This study aimed at elucidating differences between four diverse bovine NASM field strains from two species, namely S. chromogenes equorum regards to uptake (with ferritin lactoferrin as an source) siderophore production (staphyloferrin A staphyloferrin B) by...
Influenza A virus (IAV) is a single-stranded, negative-sense RNA and common cause of seasonal flu in humans. Its genome comprises eight segments that facilitate reassortment, resulting great variety IAV strains. To study these processes, the genetic code each segment should be unraveled. Fortunately, new third-generation sequencing approaches allow for cost-efficient segments. Sequencing success depends on various factors, including proper sample storage processing. Hence, this work focused...
Broad-spectrum beta-lactamase (BSBL)-producing Enterobacteriaceae impose public health threats. With increased popularity of zoos, exotic animals are brought in close proximity humans, making them important BSBL reservoirs. However, not much is known on the presence BSBLs zoos Western Europe. Fecal carriage BSBL-producing was investigated 38 zoo mammals from two Belgian zoos. Presence bla-genes using PCR, followed by whole-genome sequencing and Fourier-transform infrared spectroscopy to...
Hemorrhagic bowel syndrome (HBS) is a sporadic and fatal disease of predominantly lactating dairy cattle, characterized by segmental hemorrhage luminal clot formation in the small intestine. Although, Clostridium perfringens Aspergillus fumigatus have been associated with HBS, pathogenesis cause are currently unknown. In this study, 18 naturally occurring cases HBS (7 necropsied immediately following euthanasia, 11 12-48 hour postmortem intervals) were investigated to characterize pathology...
Abstract Actinobacillus equuli is mostly associated with disease in horses and most widely known as the causative agent of sleepy foal disease. Even though existing phenotypic tools such biochemical tests, 16S rRNA gene sequencing, Matrix Assisted Laser Desorption Ionization Time Flight Mass Spectrometry (MALDI-TOF MS) can be used to identify members genus, these methods struggle differentiate between certain species do not allow strain, virulence, antimicrobial susceptibility typing. Hence,...