A5037223609- Mosquito-borne diseases and control
- Herpesvirus Infections and Treatments
- SARS-CoV-2 and COVID-19 Research
- Viral Infections and Vectors
- Extracellular vesicles in disease
- Bartonella species infections research
- Coccidia and coccidiosis research
- Virology and Viral Diseases
- Atrial Fibrillation Management and Outcomes
- Cardiac Arrhythmias and Treatments
- Vibrio bacteria research studies
- COVID-19 Clinical Research Studies
- Veterinary medicine and infectious diseases
- MicroRNA in disease regulation
- Microbial infections and disease research
- Invertebrate Immune Response Mechanisms
- Advanced Sensor and Energy Harvesting Materials
- Congenital heart defects research
- Plant and fungal interactions
- Veterinary Equine Medical Research
- Aquaculture disease management and microbiota
- Mycotoxins in Agriculture and Food
- Angiogenesis and VEGF in Cancer
- Toxoplasma gondii Research Studies
- SARS-CoV-2 detection and testing
Ghent University
2017-2024
Ghent University Hospital
2021-2024
Salisbury University
2021
Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection is not always confined to the system, as it impacts people on a broad clinical spectrum from asymptomatic severe systemic manifestations resulting in death. Further, accumulation of intra-host single nucleotide variants during prolonged SARS-CoV-2 may lead emergence concern (VOCs). Still, information virus infectivity and evolution across organs sparse. We report detailed virological analysis thirteen postmortem disease...
Extracellular vesicles (EVs) play a possible role in cell⁻cell communication and are found various body fluids cell conditioned culture media. The aim of this study was to isolate characterize EVs medium by bovine embryos group verify if these functionally active. Initially, ultracentrifuged serum albumin (BSA) containing selected as suitable EV-free embryo medium. Next, were isolated from OptiPrepTM density gradient ultracentrifugation. Isolated characterized nanoparticle tracking analysis,...
To combat emerging infectious diseases like Zika virus (ZIKV), synthetic messenger RNAs (mRNAs) encoding viral antigens are very attractive as they allow a rapid, generic, and flexible production of vaccines. In this work, we engineered self-replicating mRNA (sr-mRNA) vaccine the pre-membrane envelope (prM-E) glycoproteins ZIKV. Intradermal electroporation few 1 µg mRNA-based ZIKV induced potent humoral cellular immune responses in BALB/c especially IFNAR1-/- C57BL/6 mice, resulting complete...
ABSTRACT Tunneling nanotubes (TNTs) are long bridge-like structures that connect eukaryotic cells and mediate intercellular communication. We found earlier the conserved alphaherpesvirus US3 protein kinase induces cell projections contact distant promote virus spread. In this report, we show US3-induced constitute TNTs. addition, report TNTs transport of information (e.g., green fluorescent [GFP]) in absence other viral proteins. remarkably stable compared to most described literature. line...
Acute hepatopancreatic necrosis disease (AHPND), a newly emergent farmed penaeid shrimp bacterial originally known as early mortality syndrome (EMS), is causing havoc in the industry. The causative agent of AHPND was found to be specific strain bacteria, e.g., Vibrio and Shewanella sps., that contains pVA1 plasmid (63–70 kb) encoding binary PirAVP PirBVP toxins. PirABVP toxins are primary virulence factors AHPND-causing bacteria mediates shrimp. Hence, this study using germ-free brine model...
Equine herpesvirus 1 (EHV1) replicates in the respiratory epithelium and disseminates through body via a cell-associated viremia leukocytes, despite presence of neutralizing antibodies. "Hijacked" previously identified as monocytic cells T lymphocytes, transmit EHV1 to endothelial endometrium or central nervous system, causing reproductive (abortigenic variants) neurological (neurological disorders. In present study, we questioned potential route infection lymphocytes how misuses vehicle...
The myocardial sleeve of the porcine and equine pulmonary veins were histologically investigated reconstructed three dimensionally. Moreover, localization neuron cell bodies at veno-atrial junction alongside was light microscopically visualized to depict organization nerve, fat tissue. Finally, presence telocytes inside demonstrated by use transmission electron microscopy. These structures are thought play a role in induction atrial fibrillation, which is frequently seen horses, while pigs...
Extracellular vesicles (EVs) have been identified as one of the communication mechanisms amongst embryos. They are secreted into embryo culture medium and, such, represent a source novel biomarkers for identifying quality cells and However, only small amounts embryo-conditioned available, which represents challenge EV enrichment. Our aim is to assess suitability different separation methods retrieve EVs with high specificity sufficient efficiency. Bovine was subjected differential...
Citrinin (CIT) is a polyketide mycotoxin occurring in variety of food and feedstuff, among which cereal grains are the most important contaminated source. Pigs poultry livestock animals frequently exposed to mycotoxins, including CIT. Concerns rising related toxic, especially potential nephrotoxic, properties The purpose this study was clarify histopathological effects on kidneys, liver, jejunum duodenum pigs, broiler chickens laying hens receiving CIT feed. During 3 weeks, pigs (n = 16)...
Ganglia and telocytes were identified inside the porcine pulmonary veins wall near myocardial sleeve tissue at atriopulmonary junction. These structures are reported to play a role in initiation of pulses from outside heart, which potentially can cause cardiac conduction disorders such as atrial fibrillation. In-depth knowledge on fine structure vein is pre-requisite better understand underlying pathophysiology fibrillation origin ectopic pulses. The importance sleeves triggering foci for...
Pseudorabies virus (PRV) is a porcine alphaherpesvirus and the causative agent of Aujeszky's disease. Successful eradication campaigns against PRV have largely relied on use potent vaccines. The live attenuated Bartha strain, which was produced by serial passaging in cell culture, represents one hallmark Despite robust protection elicited vaccination, very little known about immunogenicity strain. Previously, we showed that Bartha-infected epithelial cells trigger plasmacytoid dendritic...
Intestinal infection of many host species with Lawsonia intracellularis are widely reported. Analyses infections among carnivorous falcons have not previously been Fifty juvenile captive (Falco spp.) or without were investigated in the United Arab Emirates, including clinical laboratory methods. Fresh intestinal biopsy samples analysed by microbiological techniques for and other bacteria standard parasitological pathological was diagnosed examination qPCR 10 50 at case examination. Seven...
Abstract Background: Only seven cases of ocular Spiroplasma infection have been reported to date, all presenting as congenital cataracts with concomitant intraocular inflammation. We describe the first case initially a corneal infiltrate. Case presentation: A 1-month-old girl was referred for infiltrate in left eye. She presented our hospital unilateral keratouveitis. Examination showed stromal and dense white keratic precipitates Herpetic keratouveitis suspected intravenous acyclovir...
Abstract Background Only seven cases of ocular Spiroplasma infection have been reported to date, all presenting as congenital cataracts with concomitant intraocular inflammation. We describe the first case initially a corneal infiltrate. Case presentation A 1-month-old girl was referred for infiltrate in left eye. She presented our hospital unilateral keratouveitis. Examination showed stromal and dense white keratic precipitates Herpetic keratouveitis suspected intravenous acyclovir therapy...
Abstract The cardiac telocyte (TC) is a novel interstitial cell type with unique ultrastructure and great potential in therapy. present study examined its presence the heart of chicken embryos ageing 7–15 days old (Hamburger‐Hamilton [HH] stages 31–41) using transmission electron microscopy. TCs were identified across all atrial ventricular myocardium, close to maturing cardiomyocytes, blood vessels lymphatics. Early‐stage have immature features resembling mesenchymal cells. Late‐stage...
Abstract The coronavirus disease 2019 (COVID-19) pandemic, caused by severe acute respiratory syndrome 2 (SARS-CoV-2), has gripped the world for over a year 1,2 . SARS-CoV-2 impacts people on broad clinical spectrum from asymptomatic to and systemic manifestations resulting in death 3 In addition, intra-host genomic plasticity periodically leads emergence of new virus variants with higher transmission capacities 4–6 Autopsy series have revealed several pathways COVID-19 patients, including...