A5056035279- Veterinary medicine and infectious diseases
- Animal Virus Infections Studies
- Viral gastroenteritis research and epidemiology
- Clostridium difficile and Clostridium perfringens research
- Humic Substances and Bio-Organic Studies
- Salmonella and Campylobacter epidemiology
- Microbial infections and disease research
- Genetic and phenotypic traits in livestock
- Animal Behavior and Welfare Studies
- Animal Nutrition and Physiology
- Bird parasitology and diseases
- Mycobacterium research and diagnosis
- Coccidia and coccidiosis research
- Bioactive Compounds and Antitumor Agents
- Animal Disease Management and Epidemiology
- Virus-based gene therapy research
- Microscopic Colitis
- Helminth infection and control
- Bacteriophages and microbial interactions
- Metal Alloys Wear and Properties
- Parasitic Infections and Diagnostics
- Livestock and Poultry Management
- Vector-borne infectious diseases
- Herpesvirus Infections and Treatments
- Effects of Environmental Stressors on Livestock
Bendigo Health
2021
Tufts University
2000-2015
Universidad de Guanajuato
2014
University of Nottingham
2007-2012
University of Nottingham Ningbo China
2011
Iowa State University
1998-2005
Boehringer Ingelheim (Australia)
1998-2005
Konkuk University
2005
Meat & Livestock Australia
2004
Boehringer Ingelheim (United States)
1998-2004
A novel obligately intracellular bacterium, ileal symbiont intracellularis, which was obtained from the intestines of pigs with proliferative enteropathy disease, grown in pure cocultures tissue cultures rat cells. An examination 16S ribosomal DNA gene sequence revealed that isolates we are members delta subdivision Proteobacteria and sequences these organisms exhibit a level similarity 91% Desulfovibrio desulfuricans ATCC 27774. These were homogeneous differed cellular morphology, acid...
An obligate intracellular bacterium was isolated from the intestines of all 10 cases porcine proliferative enteropathy four different pig farms. The organism grew in a rat enterocyte cell line (IEC-18) and maintained over 20 passages. growth bacteria assessed by immunostaining cells exposed to infection. Infection not associated with morphological change, confined infected at time each transfer infection progeny these cells. is microaerophilic, dependent, curved or rod-shaped, gram-negative...
Porcine proliferative enteropathy is consistently associated with the presence of intracellular curved bacteria in epithelial cells affected portions intestine. Two strains these were cultured a cell culture system rat enterocytes (IEC-18) and passaged several times used as oral inocula for 14 gnotobiotic 8 conventional pigs. DNA immunological studies had identified belonging to new taxon, Ileal symbiont (IS) intracellularis. Conventional pigs dosed approximately 3.7 x 10(6) organisms six...
To evaluate polymerase chain reaction (PCR) for detection of Lawsonia intracellularis DNA in feces and an indirect fluorescent antibody test (IFAT) detecting serum IgG antibodies pigs exposed to L intracellularis.15 seven-week-old 42 three-week-old pigs.During 3 experiments, 23 were inoculated with a pure culture intracellularis, 31 served as noninoculated controls, used sentinels. Fecal shedding was monitored by use PCR analysis at 7-day intervals. At euthanasia, the ileum obtained...
A new genus and species of obligate intracellular bacteria found in porcine intestines are described. Growth on any bacteriological medium deprived living cells has not been demonstrated. The organism grown intracellularly cell culture. 16S rRNA gene sequence data, DNA probe results, microscopic observations provide evidence that these differ from those other described genera they belong to the delta subdivision class Proteobacteria. We have amplified sequenced ribosomal four preparations...
Abstract Objective —To evaluate the efficacy of an orally administered avirulent live vaccine to protect pigs against challenge exposure with virulent Lawsonia intracellularis . Animals —108 weaned 3-week-old (35 in experiment 1 and 73 2). Procedure —2 experiments were conducted. On day 0, vaccinates via drench or drinking water, whereas challenge-control cultured medium. 21, exposed a heterologous isolate L Clinical observations, weights, seroconversion, fecal excretion measured until 42....
Three flocks of wild sulphur-crested cockatoos in southeastern Australia had a 10-20% prevalence feather and beak deformities. In affected birds, the crest, tail, contour down feathers were lost or reduced length owing to "pinching off" 0.5-1 cm above skin. The underlying skin was discolored brown. Flight appeared normal could sustain flight. mildly only crest involved. Histological examination showed necrosis developing cells their epidermal collars. epidermis moderately hyperplastic...
Cyclical oversupply and non-profitability situations have led to pig industry consolidations in the People's Republic of China, with many smaller farmers leaving industry. In 2007, pork supply worsened due outbreaks 'high fever blue-ear disease', a complex disease issue that includes highly virulent porcine reproductive respiratory syndrome, circovirus classical swine fever. Best estimates suggest 50 million pigs were affected. More recent natural disasters (earthquakes/freezing winters)...
Proliferative enteropathy is caused by the microaerophilic obligate intracellular bacterium ileal symbiont (IS) intracellularis. Treatment of this disease problematic because lack in vivo or vitro data on activities antimicrobial agents. A new procedure for determining susceptibility IS intracellularis was developed using a tissue culture system which promotes multiplication organism. Nineteen agents were evaluated triplicate cultures their and extracellular against up to three strains...
Immunocastration is a considerable alternative to surgical castration method especially in male animal species for alleviating unwanted behaviors and characteristics. Induction of high titer antibody specific gonadotropin-releasing hormone (GnRH) correlates with the regression testes. Fusion proteins composed canine GnRH T helper (Th) cell epitope p35 originated from distemper virus (CDV) F protein goat rotavirus VP6 were produced E. coli. When these fusion injected dogs which previously...
Whole-cell and outer membrane preparations of Campylobacter mucosalis, C. hyointestinalis, jejuni, coli isolated from porcine intestines were compared with intracellular Campylobacter-like organisms extracted directly the lesions pigs proliferative enteropathy. By gradient polyacrylamide gel electrophoresis, total protein profiles significantly different each other those organisms. Immunoblotting these rabbit antisera or monoclonal antibodies prepared against showed strong reactions only a...
Campylobacter mucosalis and C hyointestinalis have been associated with the proliferative enteropathies of pigs. An examination antibody response to these organisms intracellular campylobacter-like organism was undertaken. Antibody predominantly IgM, short lived, could be detected by an immunofluorescence test using bacteria released from lesions as antigen. The majority (75 per cent) pigs enteropathy at necropsy were positive a small number (4 in which not observed found antibody. appeared...
Abstract Objective To evaluate the efficacy of orally administered tylosin phosphate for prevention and treatment proliferative enteropathy (PE) in pigs. Animals Crossbred pigs weaned at 24 days age. Procedure Pigs were challenge exposed with an inoculum Lawsonia intracellularis strain LR189/5/83- Seven control received buffer solution. Of 33 challenge-exposed pigs, 8 untreated. Two groups dosed via a 2% stabilized premix, starting 100 or 40 ppm 4 before exposure continuing 16 days, when...
The ileum, colon, and mesenteric lymph nodes of pigs naturally affected by either the two major forms proliferative enteropathy, namely, intestinal adenomatosis or hemorrhagic were examined for immunocytological responses to infection immunocytochemistry, using antibodies directed against elements porcine immune system. In both forms, there was mucosal proliferation immature enterocytes which lacked substantial histocompatibility complex class II expression a marked accumulation...