A5000195460- Hepatitis C virus research
- Liver Disease Diagnosis and Treatment
- Hepatitis B Virus Studies
- interferon and immune responses
- SARS-CoV-2 and COVID-19 Research
- COVID-19 Clinical Research Studies
- Liver physiology and pathology
- Animal Virus Infections Studies
- HIV, Drug Use, Sexual Risk
- HIV Research and Treatment
- Cytokine Signaling Pathways and Interactions
- SARS-CoV-2 detection and testing
- Virology and Viral Diseases
- Inflammatory mediators and NSAID effects
- Glycosylation and Glycoproteins Research
- Cancer Mechanisms and Therapy
- Mechanisms of cancer metastasis
- COVID-19 epidemiological studies
- COVID-19 Impact on Reproduction
- Bird parasitology and diseases
- Veterinary medicine and infectious diseases
- Escherichia coli research studies
- Long-Term Effects of COVID-19
- Cytomegalovirus and herpesvirus research
- Chromatin Remodeling and Cancer
Royal Adelaide Hospital
2019-2024
Central Adelaide Local Health Network
2021-2024
Rocky Vista University
2022
Parkview Medical Center
2022
Eastern Virginia Medical School
2018
South Australia Pathology
2008-2015
Centre for Cancer Biology
2010-2015
The University of Adelaide
2008-2015
Clinique Claude-Bernard
2015
Oregon Health & Science University
2009
The interferon-stimulated gene, viperin, has been shown to have antiviral activity against hepatitis C virus (HCV) in the context of HCV replicon, although molecular mechanisms responsible are not well understood. Here, we demonstrate that viperin plays an integral part ability interferon limit replication cell-culture-derived (JFH-1) accurately reflects complete viral life cycle. Using confocal microscopy and fluorescence resonance energy transfer (FRET) analysis, localizes interacts with...
The host protein viperin is an interferon stimulated gene (ISG) that up-regulated during a number of viral infections. In this study we have shown dengue virus type-2 (DENV-2) infection significantly induced viperin, co-incident with production RNA and via mechanism requiring retinoic acid-inducible I (RIG-I). Viperin did not inhibit DENV-2 entry but infectious release was inhibited in expressing cells. Conversely, replicated to higher tires earlier shRNA anti-DENV effect mediated by...
The interferon-induced transmembrane (IFITM) family of proteins have recently been identified as important host effector molecules the type I interferon response against viruses. IFITM1 has a potent antiviral hepatitis C virus (HCV), whereas related members IFITM2 and IFITM3 described to effects broad range RNA Here, we demonstrate that play an integral role in HCV act at level late entry stages infection. We established hepatocytes, localize early endosomes, respectively, well lysosome....
Coronavirus disease 2019 (COVID-19) convalescents living in regions with low vaccination rates rely on post-infection immunity for protection against re-infection severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2). We evaluate humoral and T cell five variants of concern (VOCs) mild-COVID-19 at 12 months after infection ancestral virus. In this cohort, ancestral, receptor-binding domain (RBD)-specific antibody circulating memory B levels are conserved most individuals, yet serum...
Host factors play an important role in all facets of the hepatitis C virus (HCV) life cycle and one such host factor is signal transducer activator transcription 3 (STAT3). The HCV core protein has been shown to directly interact with activate STAT3, while oxidative stress generated during replication a replicon-based model also induced STAT3 activation. However, despite these findings precise remains unknown. We have established that actively phosphorylated presence replicating HCV....
Hepatitis C virus (HCV) NS5A is essential for viral genome replication within cytoplasmic complexes and assembly at the lipid droplet (LD) surface, although its definitive functions are poorly understood. We developed approaches to investigate dynamics during a productive infection. report here that motility efficient HCV RNA require microtubule network motor dynein demonstrate both motile relatively static NS5A-positive foci enriched with host factors VAP-A Rab5A. Pulse-chase imaging...
The interactions between hepatitis C virus (HCV) and alcohol metabolism are not well understood. To determine the effect that has on HCV replication antiviral action of interferon (IFN), Huh-7 cells harbor metabolize ethanol via introduced expression cytochrome P450 2E1 (Cyp2e1) were treated with IFN-alpha. Treatment these (0-100 mmol/L) significantly increased replication. This was dependent Cyp2e1 alcohol-metabolized oxidative stress (OS), because antioxidant N-acetylcysteine blocked this...
A barrier to hepatitis C virus (HCV) cure is conventional testing. The aim of this study was evaluate the effect HCV antibody and RNA point-of-care testing (POCT) on rates, linkage care, treatment, acceptability in 3 priority settings Australia.
We have previously reported induction of transcriptional gene silencing (TGS) HIV-1 by short hairpin RNA (shRNA) expressed in MOLT-4 cells. The shRNA (termed shPromA) targets the highly conserved tandem NF-kB binding sequences promoter. Recent articles that TGS mediated promoter-targeted siRNAs was exclusively result sequence non-specific off-target effects. Specifically, several mismatched to target promoter were also induce significant TGS, suggesting a consequence Here, following...
Campylobacter mucosalis and C hyointestinalis have been associated with the proliferative enteropathies of pigs. An examination antibody response to these organisms intracellular campylobacter-like organism was undertaken. Antibody predominantly IgM, short lived, could be detected by an immunofluorescence test using bacteria released from lesions as antigen. The majority (75 per cent) pigs enteropathy at necropsy were positive a small number (4 in which not observed found antibody. appeared...
The pathogenesis of nonalcoholic steatohepatitis is primarily an immune-driven disease and a known factor associated with treatment failure chronic hepatitis C interferon (IFN) ribavirin. We studied the hepatocyte response in model steatosis at transcriptome level antiviral action IFN against virus (HCV) this setting. In study, we have shown that lipid loading (oleic acid palmitic acid, OA:PA) Huh-7 cells leads to increased expression classical interferon-stimulated genes (ISGs)...
Shigella flexneri targets colonic cells in humans to initiate invasive infection processes that lead dysentery, and direct interactions between their lipopolysaccharide O antigens blood group A related glycans are involved the cell adherence interactions. Here, we show treatment with Tn sialyl-Tn glycans, monoclonal antibodies lectins reactive Tn/sialyl-Tn, luteolin (a antigen synthesis inhibitor) all significantly inhibited S. invasion of vitro. Surface plasmon resonance analysis showed had...
COVID-19 vaccination represents a key preventative part of the Australian public health approach to severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) pandemic. Hospital inpatients are frequently high risk for and death. Anecdotes high-risk being unvaccinated lack electronic medical record (EMR) visibility status prompted present study as these patients could represent themselves, staff, other service provision.To determine uptake vaccine among at an adult tertiary hospital...
Background: Point-of-care hepatitis C virus (HCV) testing streamlines and treatment pathways. In this study, we established an HCV model of care in a homelessness service by offering antibody RNA point-of-care testing. Methods: A nurse peer-led with peer support were implemented between November 2021 April 2022 at Adelaide, Australia. All clients the eligible to participate. Clients offered initial test, positive immediately who tested linked viral for treatment. Results: total 230 received...
The intrahepatic cholangiocyte organoids (ICOs) model was evaluated for host differences in hepatitis B virus (HBV) infection, cellular responses, antiviral and immunomodulator responses. Twelve ICOs generated from liver resections biopsies were assessed metabolic markers functional HBV entry receptor expression throughout differentiation. Structural changes relevant to infection characterized using histology, confocal, electron microscopy examinations. Optimal ICO culture conditions HepAD38...
Background: Duration and quality of immunity to SARS-CoV-2 have significant implications for the management COVID-19 pandemic. Here, we present a comprehensive set immunological data from cohort individuals (n=43), 12 months after mild disease in absence virus re-exposure.Methods: Serum PBMC were collected mild-COVID-19 convalescents positive PCR (n=43) healthy SARS-CoV-2-seronegative controls (n=15). titers SARS-CoV-2-specific immunoglobulins quantified by ELISA neutralisation activity was...
Abstract Background Newer antiviral agents for chronic hepatitis B (CHB) are highly effective, with minimal risks of complications and development resistance. Aim To identify the proportion patients CHB on treatment who will not require alteration management clinical factors those closer monitoring. Methods Patients were entecavir and/or tenofovir between January 2011 December 2016 retrospectively studied. According to initial plan provided by managing physician, any deviation in interval...
Proximal epithelioid sarcomas are rare soft tissue that have been documented in a diverse range of presentations. However, there few cases describing adrenal These neoplasms thought to be driven by loss SWItch/sucrose non-fermentable (SWI/SNF)-related matrix-associated actin-dependent regulator chromatin subfamily B member 1 (SMARCB1), also known as integrase interactor (INI1). SMARCB1/INI1 is tumor suppressor gene play role multiple malignancies with varying degrees expression. Complete has...