A5074589598- Veterinary medicine and infectious diseases
- Humic Substances and Bio-Organic Studies
- Bioactive Compounds and Antitumor Agents
- Streptococcal Infections and Treatments
- Plant Pathogenic Bacteria Studies
- Salmonella and Campylobacter epidemiology
- Metal Alloys Wear and Properties
- Coccidia and coccidiosis research
- Neonatal and Maternal Infections
- Clostridium difficile and Clostridium perfringens research
- Viral gastroenteritis research and epidemiology
- Animal Nutrition and Physiology
- Microscopic Colitis
- Parasitic Infections and Diagnostics
- Pharmacological Effects of Natural Compounds
- Infections and bacterial resistance
- Mycobacterium research and diagnosis
- Advanced ceramic materials synthesis
- Animal Virus Infections Studies
- Microbial infections and disease research
- Gastrointestinal disorders and treatments
- Toxin Mechanisms and Immunotoxins
- Infective Endocarditis Diagnosis and Management
- Inflammatory Bowel Disease
- Leptospirosis research and findings
University of Minnesota
2014-2023
University of Minnesota System
2013-2022
Twin Cities Orthopedics
2008-2019
University of Nebraska Medical Center
2018
University of California, Davis
2011
Hagyard Equine Medical Institute
2011
University of Kentucky
2011
Pfizer (United States)
2002
University of Minnesota Medical Center
1995-1996
Collins College
1993
A novel obligately intracellular bacterium, ileal symbiont intracellularis, which was obtained from the intestines of pigs with proliferative enteropathy disease, grown in pure cocultures tissue cultures rat cells. An examination 16S ribosomal DNA gene sequence revealed that isolates we are members delta subdivision Proteobacteria and sequences these organisms exhibit a level similarity 91% Desulfovibrio desulfuricans ATCC 27774. These were homogeneous differed cellular morphology, acid...
A sensitive assay based on amplification of a 319-bp DNA fragment the intracellular bacterium swine proliferative enteritis was developed for detection organism in feces swine. vernacular name, ileal symbiont intracellularis (IS-intracellularis), has recently been published bacterium, which formerly known as Campylobacter-like (C.J. Gebhart, S.M. Barnes, S. McOrist, G.F. Lin, and G.H.K. Larson, Int. J. Syst. Bacteriol. 43:533-538, 1993). As few 10(1) IS-intracellularis organisms purified...
Streptococcus suis is a swine pathogen and zoonotic agent afflicting people in close contact with infected pigs or pork meat. Sporadic cases of human infections have been reported worldwide. In addition, S. outbreaks emerged Asia, making this bacterium primary health concern part the globe. pigs, disease results decreased performance increased mortality, which significant economic impact on production Facing new regulations preventive use antimicrobials livestock lack effective vaccines,...
During the course of studies to reproduce proliferative enteritis in hamsters, Campylobacter cinaedi was recovered from feces majority healthy hamsters obtained two commercial sources. The organisms were cultured by using filtration, a nonselective medium, and microaerophilic atmosphere containing hydrogen. Isolation hindered fastidious nature C. presence other species hamster intestine. All isolates phenotypically similar ATCC 35683. Comparison whole-cell protein profiles one isolate with...
The name "Campylobacter hyointestinalis" sp. nov. is proposed for a Campylobacter species that was isolated from the intestines of pigs with proliferative enteritis. "C. also found in feces cattle and has been intestine hamster. distinguished previously described catalase-positive by colony morphology, ability to produce H2S triple sugar iron agar, grow anaerobically 0.1% trimethylamine N-oxide hydrochloride, resistance nalidixic acid, susceptibility cephalothin metronidazole, hydrogenase...
SUMMARY Intestines from 48 swine with enteric disease were examined by bacteriologic cultural technique for the presence of various Campylobacter species. Histopathologic techniques used to determine whether submitted specimens had lesions either proliferative ileitis or other diseases. Three species identified as jejuni/coli , sputorum ss mucosalis and hyointestinalis (proposed new species) on basis biochemical characteristics response inhibitory substances. The C was isolated 18 27 (67%)...
Since 2007, outbreaks of severe bloody diarrhea and hemorrhagic colitis have been reported in the United States Canada. Though primary causative agent swine dysentery is Brachyspira hyodysenteriae, which strongly hemolytic, current report describes isolation a novel hemolytic sp. This was identified from clinical submissions at Minnesota Veterinary Diagnostic Laboratory, 40 such isolates were obtained 22 representing 5 states. Isolates confirmed to be different any known on basis...
Salmonella enterica is a leading cause of food borne illness. Recent studies have shown that S. pathogen capable causing alterations to the composition intestinal microbiome. A recent prospective study French pork production farms found statistically significant association between Lawsonia intracellularis and carriage enterica. In current gut microbiome was determined in pigs challenged with serovar Typhimurium or L. compared non-challenged control pigs. Principal coordinate analysis...
Streptococcus suis is a significant cause of mortality in piglets and growing pigs worldwide. The species contains pathogenic commensal strains, with strains causing meningitis, arthritis, endocarditis, polyserositis, septicemia. Serotyping multilocus sequence typing (MLST) are primary methods to differentiate but the information limited for found United States. objective this study was characterize diversity 208 S. isolates collected between 2014 2017 across North America (mainly States) by...
Proliferative enteropathy (PE) is a transmissible enteric disease caused by Lawsonia intracellularis. An outbreak of equine PE was diagnosed in foals from 3 breeding farms. Most had been weaned prior to the appearance clinical signs, which included depression, rapid and marked weight loss, subcutaneous oedema, diarrhoea colic. Poor body condition with rough haircoat potbellied were common findings affected foals. Respiratory tract infection, dermatitis intestinal parasitism also found some...
A new genus and species of obligate intracellular bacteria found in porcine intestines are described. Growth on any bacteriological medium deprived living cells has not been demonstrated. The organism grown intracellularly cell culture. 16S rRNA gene sequence data, DNA probe results, microscopic observations provide evidence that these differ from those other described genera they belong to the delta subdivision class Proteobacteria. We have amplified sequenced ribosomal four preparations...
The sensitivity and specificity of an immunoperoxidase monolayer assay (IPMA) was evaluated in a blind serologic study group disease-free pigs experimentally infected with intestinal homogenate containing Lawsonia intracellulars organisms. Sixty from the control were kept source farm, another 60 animals transferred to isolation unit challenged intragastrically. All bled before 21 days after challenge. Fecal samples collected on same dates. IPMA results tested for 2 × table using...
Proliferative enteropathy (proliferative enteritis, proliferative ileitis, intestinal adenomatosis) has been reported in several animal species including the pig,1 dog, foal, blue fox, guinea pig, ferret, hamster, and rabbit. The disease is characterized by adenomatous hyperplasia of crypt epithelial cells ileum colon with intracytoplasmic curved bacteria resembling Campylobacter species. In single case previously a Campylobacter-like organisms were demonstrated within cytoplasm enterocytes...
The objective of this study was to determine whether Lawsonia intracellularis present in the feces free-living animals collected on two equine premises with documented occurrence proliferative enteropathy (EPE). Fresh from black-tailed jackrabbits (Lepus californicus, n=100), striped skunks (Mephitis mephitis, n=22), feral cats (Felis catus, n=14), Brewer's Blackbirds (Euphagus cyanocephalus, n=10), Virginian opossums (Didelphis virginiana, n=9), raccoons (Procyon lotor, n=4), California...
Lawsonia intracellularis is an obligate intracellular bacterium and the etiologic agent of proliferative enteropathy. The disease endemic in pigs, emerging horses has been described various other species including nonhuman primates. Cell proliferation associated with bacterial replication enterocyte cytoplasm, but molecular basis host-pathogen interaction unknown. We used laser capture microdissection coupled RNA-seq technology to characterize transcriptional responses infected enterocytes...
ABSTRACT Swine dysentery (SD) is a mucohemorrhagic colitis of swine classically caused by infection with the intestinal spirochete Brachyspira hyodysenteriae . Since around 2007, cases SD have occurred in North America associated different strongly beta-hemolytic that has been molecularly and phenotypically characterized provisionally named “ hampsonii. ” Despite increasing international interest, B. hampsonii currently not recognized as valid species. To support its recognition, we...
P roliferative enteritis (PE) or enteropathy is an enteric disease that develops in a number of animals.Much the literature focuses on swine I and hamsters.2 Indeed, substantial problem estimated to cost industry $20/sow annually Australia 3 $20 million United States' Between 15 30% herds have been be affected endemic areas, with 5 20% infection rate within herd.'•6Proliferative was reported as important laboratory-reared hamsters (commonly referred wet-tail hamsters) from 1960s 1980s.2...
Equine proliferative enteropathy (EPE) is an emerging disease of weanling foals.Describe clinical, hematologic, biochemical, serologic, molecular, and ultrasonographic findings in foals experimentally infected with Lawsonia intracellularis.Eight foals.Recently weaned were assigned to either the challenge (n = 3), sentinel or control 2) group. Foals challenged via intragastric inoculation 3 x 10(10)L. intracellularis organisms grown culture. Each foal was housed a order assess feco-oral...