A5046547393- Mosquito-borne diseases and control
- Viral Infections and Vectors
- Malaria Research and Control
- Vector-borne infectious diseases
- Virology and Viral Diseases
- Trypanosoma species research and implications
- Viral gastroenteritis research and epidemiology
- Insect and Pesticide Research
- SARS-CoV-2 and COVID-19 Research
- Respiratory viral infections research
- Radiation Effects and Dosimetry
- Vector-Borne Animal Diseases
- Animal Virus Infections Studies
- Microbial Metabolic Engineering and Bioproduction
- Listeria monocytogenes in Food Safety
- Research on Leishmaniasis Studies
- Immunotherapy and Immune Responses
- RNA Interference and Gene Delivery
- COVID-19 Clinical Research Studies
- Rabies epidemiology and control
- Insect symbiosis and bacterial influences
- Enterobacteriaceae and Cronobacter Research
- Microbial Inactivation Methods
- Biopolymer Synthesis and Applications
- Nuclear Structure and Function
Fraunhofer Institute for Cell Therapy and Immunology
2016-2025
Sechenov University
2019-2021
Universidade Federal da Bahia
2021
Charité - Universitätsmedizin Berlin
2019-2021
Humboldt-Universität zu Berlin
2019
Freie Universität Berlin
2019
Fundação Oswaldo Cruz
2019
German Center for Infection Research
2019
D’Or Institute for Research and Education
2019
Fraunhofer Society
2008-2012
During 2015 to 2016, Brazil reported more Zika virus (ZIKV) cases than any other country, yet population exposure remains unknown. Serological studies of ZIKV are hampered by cross-reactive immune responses against heterologous viruses. We conducted serosurveys for ZIKV, dengue (DENV), and Chikungunya (CHIKV) in 633 individuals prospectively sampled during including microcephaly non-microcephaly pregnancies, HIV-infected patients, tuberculosis university staff Salvador northeastern using...
To combat emerging infectious diseases like Zika virus (ZIKV), synthetic messenger RNAs (mRNAs) encoding viral antigens are very attractive as they allow a rapid, generic, and flexible production of vaccines. In this work, we engineered self-replicating mRNA (sr-mRNA) vaccine the pre-membrane envelope (prM-E) glycoproteins ZIKV. Intradermal electroporation few 1 µg mRNA-based ZIKV induced potent humoral cellular immune responses in BALB/c especially IFNAR1-/- C57BL/6 mice, resulting complete...
Abstract Background The bacterium Coxiella burnetii is the etiological agent of Q fever and mainly transmitted via inhalation infectious aerosols. DNA C. frequently detected in ticks, but role ticks as vectors epidemiology this still controversial. In study, Ixodes ricinus Dermacentor marginatus adults well I. nymphs were fed on blood spiked with order to study fate within putative tick vectors. Methods Blood-feeding experiments performed vitro silicone-membrane based feeding units. uptake,...
Monitoring the humoral protective immune response and its durability after SARS-CoV-2 infections is essential for risk assessment of reinfections, improvement diagnostic methods evaluation vaccine trials. We have analyzed neutralizing antibodies IgG responses specific to different antigens, including inactivated whole-virion SARS-CoV-2, spike subunit 1 protein receptor binding domain, as well nucleocapsid protein. show dynamic developments from early convalescent stages up 9 months post...
Among the earliest events in postmitotic nuclear envelope (NE) assembly are interactions between chromatin and membranes that will fuse to form NE. It has been proposed integral NE proteins mediate initial membrane recruitment chromatin. We show several transmembrane bind DNA directly as a class enriched long, basic domains potentially DNA. Membrane fractions essential for formation shown protein-free DNA, our data suggest these critical early steps assembly.
Inactivated vaccines are commonly produced by incubating pathogens with chemicals such as formaldehyde or β-propiolactone. This is a time-consuming process, the inactivation efficiency displays high variability and extensive downstream procedures often required. Moreover, application of alters antigenic components viruses bacteria, resulting in reduced antibody specificity therefore stimulation less effective immune response. An alternative method for ionizing radiation. It acts very fast...
A central aspect of current virology is to define the function cellular proteins (host factors) that support viral multiplication process. This study aimed at characterizing assist RNA replication process prevalent human pathogen West Nile virus (WNV). Using in vitro and cell-based approaches, we defined p45 isoform AU-rich element RNA-binding protein 1 (AUF1) as a host factor enables efficient WNV replication. It was demonstrated AUF1 has an chaperone activity, which aids structural...
ABSTRACT This study demonstrated that West Nile virus (WNV) excreted in the urine of patients with acute infection can be isolated cell cultures. In addition, protocols for WNV isolation from samples were standardized, and factors may affect efficiency identified.
Detection of antibodies is widely used for the diagnosis infections with arthropod-borne flaviviruses including dengue (DENV) and Zika virus (ZIKV). Due to emergence ZIKV in areas endemic DENV, massive co-circulation observed methods specifically diagnose these differentiate them from each other are mandatory. However, serological assays general, DENV particular, compromised by high degree similarities their proteins which can lead cross-reacting false-positive test results. Cross-reacting...
The currently used SARS-CoV-2 mRNA vaccines have proven to induce a strong and protective immune response. However, functional relevance of vaccine-generated antibodies their temporal progression are still poorly understood. Thus, the central aim this study is gain better understanding systemic mucosal humoral response after vaccination with BNT162b2.We compared antibody production against S1 subunit RBD spike protein in sera BNT162b2 vaccinees, heterologous ChAdOx1-S/BNT162b2 vaccinees...
In 2019, the novel highly infectious severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) outbreak rapidly led to a global pandemic with more than 346 million confirmed cases worldwide, resulting in 5.5 associated deaths (January 2022). Entry of all SARS-CoV-2 variants is mediated by cellular angisin-converting enzyme (ACE2). The virus abundantly replicates epithelia upper tract. Beyond vaccines for immunization, there an imminent need treatment options COVID-19 patients. So far,...
The respiratory syncytial virus (RSV) is a leading cause of acute lower tract infections associated with numerous hospitalizations. Recently, intramuscular (i.m.) vaccines against RSV have been approved for elderly and pregnant women. Noninvasive mucosal vaccination, e.g., by inhalation, offers an alternative pathogens like RSV. Effective induce local immune responses, potentially resulting in the efficient fast elimination viruses after natural infection. To investigate this response to...
The inner nuclear membrane (INM) of eukaryotic cells is characterized by a unique set transmembrane proteins which interact with chromatin and/or the lamina. number identified INM steadily increasing, mainly as result proteomic and computational approaches. However, despite link between mutation several these disease, function most remains unknown depletion many from variety systems did not produce an obvious phenotype in affected cells. Here, we report that conserved protein Lem2 human cell...
In seroconversion panels obtained from patients Brazil, diagnostic testing for Zika virus infection was improved by combining multiple antibody isotypes, techniques, and antigens, but sensitivity remained suboptimal. contrast, chikungunya unambiguous. Recurrent recent arbovirus infections suggested serologic data unspecific symptoms highlight the need exhaustive virologic testing.