A5082633163- Antifungal resistance and susceptibility
- Fungal Infections and Studies
- Bacterial Identification and Susceptibility Testing
- Identification and Quantification in Food
- Metabolomics and Mass Spectrometry Studies
- Enzyme Structure and Function
- Fungal and yeast genetics research
- DNA Repair Mechanisms
- Advanced Proteomics Techniques and Applications
- Collagen: Extraction and Characterization
- Cancer, Hypoxia, and Metabolism
- Carbohydrate Chemistry and Synthesis
- Fungal Biology and Applications
- Viral gastroenteritis research and epidemiology
- Listeria monocytogenes in Food Safety
- Metabolism, Diabetes, and Cancer
- Diphtheria, Corynebacterium, and Tetanus
- Gene expression and cancer classification
- Microbial Metabolites in Food Biotechnology
- Salmonella and Campylobacter epidemiology
- Peptidase Inhibition and Analysis
- Invertebrate Immune Response Mechanisms
- RNA and protein synthesis mechanisms
- Entomopathogenic Microorganisms in Pest Control
- Bacterial biofilms and quorum sensing
Chemisches und Veterinäruntersuchungsamt Stuttgart
2020-2024
Fraunhofer Institute for Interfacial Engineering and Biotechnology
2008-2017
Fraunhofer Society
2012
University of Stuttgart
2007-2011
University of Konstanz
2003-2004
Rosalind Franklin University of Medicine and Science
1988
The opportunistic fungal pathogen Candida glabrata is a frequent cause of candidiasis, causing infections ranging from superficial to life-threatening disseminated disease. inherent tolerance C. azole drugs makes this serious clinical threat. To identify novel genes implicated in antifungal drug tolerance, we have constructed large-scale deletion library consisting 619 unique, individually bar-coded mutant strains, each lacking one specific gene, all together representing almost 12% the...
The cell wall of the human-pathogenic fungus Candida albicans is a robust but also dynamic structure which mediates adaptation to changing environmental conditions during infection. Sap9 and Sap10 are surface-associated proteases function in C. integrity interaction with human epithelial cells neutrophils. In this study, we have analyzed enzymatic properties investigated whether these cleave proteins on fungal surface. We show that Sap10, contrast other aspartic proteases, exhibit...
The SUN gene family has been defined in Saccharomyces cerevisiae and comprises a fungus-specific of proteins which show high similarity their C-terminal domains. Genes this are involved different cellular processes, like DNA replication, aging, mitochondrial biogenesis, cytokinesis. In Candida albicans the two genes, SUN41 SIM1. We demonstrate that C. mutants lacking similar defects as found for S. cerevisiae, including addition, mutant showed higher sensitivity towards cell wall-disturbing...
The human fungal pathogen Candida glabrata is related to Saccharomyces cerevisiae but has developed high resistance against reactive oxygen species. We find that induction of conserved genes encoding antioxidant functions dependent on the transcription factors CgYap1 and CgSkn7 which cooperate for promoter recognition. Superoxide stress C. provided by superoxide dismutase CgSod1, not CgYap1/Skn7. Only double mutants lacking both CgSod1 were efficiently killed primary mouse macrophages. Our...
Inactivated vaccines are commonly produced by incubating pathogens with chemicals such as formaldehyde or β-propiolactone. This is a time-consuming process, the inactivation efficiency displays high variability and extensive downstream procedures often required. Moreover, application of alters antigenic components viruses bacteria, resulting in reduced antibody specificity therefore stimulation less effective immune response. An alternative method for ionizing radiation. It acts very fast...
We have determined the nucleotide sequence of gene for fructose-1,6-bisphosphatase from both Saccharomyces cerevisiae and Schizosaccharomyces pombe. The predicted protein S. contains 347 amino acids has a molecular weight 38,100; that pombe, 346 38,380. Comparison these acid sequences with each other pig kidney shows several regions strong homology separated by divergence. These homologous are likely candidates functional domains. A cassette was constructed expressed regulated PHO5 GAL1...
The majority of microorganisms persist in nature as surface-attached communities often surrounded by an extracellular matrix, called biofilms. Most natural biofilms are not formed a single species but multiple species. Microorganisms only cooperate some multispecies also compete for available nutrients. Gram-negative bacterium Pseudomonas aeruginosa and the polymorphic fungus Candida albicans two opportunistic pathogens that found coexisting human host. Several models mixed have been...
ABSTRACT Fungal infections are a leading cause of morbidity and death for hospitalized patients, mainly because they remain difficult to diagnose treat. Diseases range from widespread superficial such as vulvovaginal life-threatening systemic candidiasis. For mycoses, only restricted arsenal antifungal agents is available. Commonly used classes compounds include azoles, polyenes, echinocandins. Due emerging resistance standard therapies, significant side effects, high costs several...
ABSTRACT Studying infectious diseases requires suitable hosts for experimental in vivo infections. Recent years have seen the advent of many alternatives to murine infection models. However, use non-mammalian models is still controversial because it often unclear how well findings from these systems predict virulence potential humans or other mammals. Here, we compare commonly used models, fruit fly and mouse (representing invertebrate mammalian hosts), their similarities degree correlation...
Analysing culture supernatants of yeast and hyphal cells Candida albicans, we found two close homologues pathogenesis-related (PR-) 1 proteins, Rbe1p Rbt4p, in the secretome. Due to sequence homology, three additional, yet not characterized open reading frames, ORF19.6200, ORF19.2787 ORF19.2336, together with RBE1 RBT4 were assigned a novel family CaPRY proteins. In Δrbe1/Δrbt4 deletion strain, genome-wide transcriptional analysis revealed differential transcription only limited set genes...
The properties of dephospho- and phosphofructose-1,6-bisphosphatase from the yeast Saccharomyces cerevisiae two mutant enzymes in which phosphorylatable Ser11 had been changed by site-directed mutagenesis (Ser—-Ala Ser—-Asp) were studied to clarify role cyclic AMP-dependent phosphorylation fructose-1,6-bisphosphatase. wild type fructose-1,6-bisphosphatase overexpressed purified homogeneity. Phosphofructose-1,6-bisphosphatase was prepared vitro phosphorylation. comparison above demonstrated...
Corynebacterium pseudotuberculosis (CPS) is an important bacterial animal pathogen. CPS causes chronic, debilitating and currently incurable infectious diseases affecting a wide range of livestock wild herbivores including camelids worldwide. Belonging to the diphtheria e complex, this pathogen can also infect humans. The classical characterization typically based on testing nitrate reductase activity, separating two biovars Equi Ovis. However, more refined resolutions are required unravel...
Micro-organisms sense the availability of nutrients in their environment to control cellular behaviour and expression transporters enzymes that are required for utilization these nutrients. In pathogenic yeast Candida albicans, preferred nitrogen source ammonium suppresses switch from filamentous growth response certain stimuli, it also represses secretion proteases, which proteins as an alternative source. To investigate whether C. albicans senses extracellular or if uptake into cell is...
Abstract Microbial transglutaminase (TG) is an enzyme isolated on industrial scale from Streptomyces mobaraensis . Technical TG, a formulated powder, primarily used to restructure meat in the meat-processing industry, typically at 1% concentration and often referred as “meat glue.” In European Union, restructured with TG requires indication “formed meat” label according Regulation (EU) No 1169/2011. order detect food fraud like undeclared usage products, qualitative mass spectrometric method...
For successful elucidation of a food-borne infection chain, the availability high-quality sequencing data from suspected microbial contaminants is prerequisite. Commonly, those investigations are joint effort undertaken by different laboratories and institutes. To analyze extent variability introduced differing wet-lab procedures on quality sequence we conducted an interlaboratory study, involving four bacterial pathogens, which account for majority food-related infections: Campylobacter...
We describe the animal species identification of meat using MALDI-TOF mass spectroscopy including development and validation a reliable method, qualified for use in accredited official food-control laboratory. Previous publications had shown potential MS differentiation several kind food, meat. Our aim was to establish rapid method by means simplified sample preparation without prior tryptic digest, an existing popular MALDI system, independent extensive reference database, adequate concept....
<p>We describe the animal species identification of meat using MALDI-TOF mass spectroscopy including development and validation a reliable method, qualified for use in accredited official food-control laboratory.</p> <p>Previous publications had shown potential MS differentiation several kind food, meat. Our aim was to establish rapid method by means simplified sample preparation without prior tryptic digest, an existing popular MALDI system, independent extensive reference...
Since its development, microarray technology has evolved to a standard method in the biotechnological and medical field with broad range of applications. Nevertheless, underlying mechanism hybridization process PCR-products capture probes is still not completely understood, several observed phenomena cannot be explained current models. We investigated influence parameters on reaction identified ssDNA play major role process. An increase content strongly enhanced resulting signal intensities....