A5006838699- Metal Extraction and Bioleaching
- Minerals Flotation and Separation Techniques
- Extraction and Separation Processes
- Mine drainage and remediation techniques
- Microbial Community Ecology and Physiology
- Protist diversity and phylogeny
- Methane Hydrates and Related Phenomena
- Radiation Effects and Dosimetry
- Listeria monocytogenes in Food Safety
- Photosynthetic Processes and Mechanisms
- Respiratory viral infections research
- Neonatal Respiratory Health Research
- Legume Nitrogen Fixing Symbiosis
- Innovative Microfluidic and Catalytic Techniques Innovation
- Enzyme Production and Characterization
- Insect and Pesticide Research
- Molecular Biology Techniques and Applications
- Radiation Shielding Materials Analysis
- Parasitic Infections and Diagnostics
- Diatoms and Algae Research
- Toxoplasma gondii Research Studies
- Nuclear Physics and Applications
- Anaerobic Digestion and Biogas Production
- Carcinogens and Genotoxicity Assessment
- Mosquito-borne diseases and control
Fraunhofer Institute for Organic Electronics, Electron Beam and Plasma Technology
2022-2024
GWT-TUD (Germany)
2024
TU Bergakademie Freiberg
2014-2019
University of Regensburg
2008-2014
The respiratory syncytial virus (RSV) is a leading cause of acute lower tract infections associated with numerous hospitalizations. Recently, intramuscular (i.m.) vaccines against RSV have been approved for elderly and pregnant women. Noninvasive mucosal vaccination, e.g., by inhalation, offers an alternative pathogens like RSV. Effective induce local immune responses, potentially resulting in the efficient fast elimination viruses after natural infection. To investigate this response to...
The aim of this study was to investigate the potential bioleaching for treatment an environmentally hazardous waste, a blast‐furnace flue dust designated Theisen sludge. Bioleaching sludge investigated at acidic conditions with Acidithiobacillus ferrooxidans in pure and mixed‐species culture Acidiphilium. In shaking‐flask experiments, parameters (pH, redox potential, zinc extraction from ZnS, ferrous‐ ferric‐iron concentration) were controlled regularly. analysis dissolved metals showed that...
Radiation-attenuated intracellular parasites are promising immunization strategies. The irradiated able to invade host cells but fail fully replicate, which allows for the generation of an efficient immune response. Available radiation technologies such as gamma rays require complex shielding constructions and difficult be integrated into pharmaceutical production processes. In this study, we evaluated first time low-energy electron irradiation (LEEI) a method generate replication-deficient...
The surfaces of 8 bacterial and 23 archaeal species, including many hyperthermophilic Archaea, could be stained using succinimidyl esters fluorescent dyes. This allowed us for the first time to analyze mode cell wall growth in Archaea by subculturing cells. data obtained show that incorporation new material follows pattern observed Bacteria: coccoid species Pyrococcus furiosus was region septum formation while rod-shaped Methanopyrus kandleri Methanothermus sociabilis, a diffuse over length...
We have described previously that the flagella of Euryarchaeon Pyrococcus furiosus are multifunctional cell appendages used for swimming, adhesion to surfaces and formation cell-cell connections. Here, we characterize these organelles with respect their biochemistry transcription. Flagella were purified by shearing from cells followed CsCl-gradient centrifugation found consist mainly a ca. 30 kDa glycoprotein. Polymerization studies denatured resulted in an ATP-independent flagella-like...
Respiratory syncytial virus (RSV) is a leading cause of acute lower respiratory tract infections in the elderly and children, associated with pediatric hospitalizations. Recently, first vaccines have been approved for people over 60 years age applied by intramuscular injection. However, vaccination route via mucosal application holds great potential protection against pathogens like RSV. Mucosal induce local immune responses, resulting fast efficient elimination viruses after natural...
Low-energy electron beam irradiation, which refers to the treatment of matter with accelerated electrons at energies below 300 keV, is gaining attention for many applications in life sciences. In scope vaccine production, low-energy irradiation an efficient and chemical free technology inactivate viruses. has also been proposed be a powerful technique its use biotechnological processes. As operate aqueous environment, there urgent need accurate dose quantification liquids, especially low (<...
Low-energy electron irradiation is an emerging alternative technology for attenuated or complete pathogen inactivation with respect to medical, biotechnological, and pharmaceutical applications. Pathogen by ionizing radiation depends mainly on the absorbed dose. In low-energy processes, determination of dose challenging due limited, material-dependent penetration depth accelerated electrons into matter. general, there are established dosimetry systems evaluate under dry conditions. However,...
The model organism Pyrococcus furiosus has recently been reported to interact with Methanopyrus kandleri in coculture, suggesting a H 2 symbiosis. In the current study we further investigated this hypothesis by growing P. four other hyperthermophilic methanogens providing evidence that organisms did not only exert positive effects ( furiosus/ Methanocaldococcus villosus and infernus) on each other, but also neutral jannaschii) even inhibitory interactions Methanotorris igneus) were detected...
Tick-borne encephalitis virus (TBEV) is a zoonotic flavivirus which endemic in many European and Asian countries. Humans can get infected with TBEV usually via ticks, possible symptoms of the infection range from fever to severe neurological complications such as encephalitis. Vaccines protect against TBEV-induced disease are widely used most them consist whole viruses, inactivated by formaldehyde. Although this production process well established, it has several drawbacks, including usage...
During the process of industrialization, world-wide demand for resources steadily increases. Significant amounts crucial metals and metalloids remain in low grade mineral deposits, however using conventional metal extraction methods on these minerals is not environmentally feasible. To overcome problems, biohydrometallurgy has become focus recent research. Biohydrometallurgy utilizes activity iron-oxidizing bacteria to catalyze dissolution sulfide minerals, our case local sphalerite (ZnS),...
Theisen sludge, a fine-grained by-product from copper smelting is secondary resource for the winning of strategic elements by metal-oxidizing acidophilic bacteria. Screening cultures, that grew in presence toxic heavy metals containing fly ash was successfully performed. Small scale experiments shaking flasks with subsequent analysis pregnant leaching solution (PLS) revealed biological extraction major (zinc and copper) minor (cobalt rhenium). Results were confirmed XRF analyses residue. The...
Here, we communicate the draft genome of "Acidibacillus ferrooxidans" Huett2, a novel strain an acidophilic, heterotrophic, iron-oxidizing bacterium belonging to phylum Firmicutes It was isolated from water drainage system former minefield in Freiberg, Germany.
Abstract Accelerating electrons to achieve chemical and biological effects is a well-established competence of Fraunhofer FEP. Today, there large variety low-energy electron beam applications with broad range absorbed doses, e.g. modification plastics, plasma-chemical syntheses, pollutant removal in wastewaters exhaust gases, sterilization medical products, disinfection seeds, biocompatible functionalization implants stimulation biotechnological processes. This calls for reliable, sensitive,...
Abstract In recent years, the use of low-energy electrons in various ecological and biotechnological applications has become increasingly relevant. One important application is treatment wastewater, wherein highly reactive species produced by water irradiation are used to oxidize pollutants. Low-energy electron several advantages, such as minimal demands on radiation protection beam (EB) source dimensions. However, play into main advantages this technology keep it economically viable,...
In this study, a novel bead-based dosimetry system was developed. This approach uses suspended polymethyl methacrylate beads, which experience dose-dependent change in fluorescence intensities. The beads were diluted deionized water and exposed to low-energy electron irradiation. Afterward, the analyzed by flow cytometry. mean of these increased with radiation dose range 10–50 kGy. Thus, cytometric measurements allow mapping: signal each bead could be assigned corresponding dose. correlation...
Abstract An expanding part of electron beam technology developed at Fraunhofer FEP tackles ecological and biotechnological challenges highest relevance. Antimicrobial fungicidal effects are exploited in the chemical-free phytosanitary treatment seeds. Chemical pollutants but also pathogens gaseous effluents waste water can be efficiently neutralized. Medical products such as tools, packaging implants sterilized, disinfected, or even biologically advantageously functionalized. Viruses...
Recently, a novel acidophilic heterotrophic iron oxidizing bacterium belonging to the newly described genus Acidibacillus (formerly Alicyclobacillus ) was isolated from water drainage ditch in Freiberg, Germany. Bioleaching tests showed that ferrooxidans Huett2 contributes dissolution of minerals. As microbe-mineral interactions play crucial role nature and enhance reaction kinetics mineral dissolution, attachment Ab. on sulfide minerals pyrite (FeS 2 ), chalcopyrite (CuFeS chalcocite (Cu S)...