A5074190377- Microbial infections and disease research
- Bacteriophages and microbial interactions
- Aquaculture disease management and microbiota
- Genomics and Phylogenetic Studies
- Salmonella and Campylobacter epidemiology
- Animal Behavior and Welfare Studies
- Viral gastroenteritis research and epidemiology
- Plant and Fungal Interactions Research
- Bacterial Identification and Susceptibility Testing
- Streptococcal Infections and Treatments
- Animal Disease Management and Epidemiology
- Animal Virus Infections Studies
- Rabies epidemiology and control
- Plant Disease Resistance and Genetics
- Escherichia coli research studies
- Insect and Pesticide Research
- Bacterial Genetics and Biotechnology
- Plant Pathogenic Bacteria Studies
- Antimicrobial Resistance in Staphylococcus
- Enterobacteriaceae and Cronobacter Research
- Herpesvirus Infections and Treatments
- Veterinary medicine and infectious diseases
- Listeria monocytogenes in Food Safety
- Mycobacterium research and diagnosis
- Milk Quality and Mastitis in Dairy Cows
University of Bern
2016-2025
Federal Food Safety and Veterinary Office
2017
University of Veterinary Medicine Hannover, Foundation
2011
University Hospital of Geneva
2007
University of Zurich
1999
Weizmann Institute of Science
1994
ABSTRACT A disposable microarray was developed for detection of up to 90 antibiotic resistance genes in gram-positive bacteria by hybridization. Each gene is represented two specific oligonucleotides chosen from consensus sequences families, except nine which only one oligonucleotide could be developed. total 137 (26 33 nucleotides length with similar physicochemical parameters) were spotted onto the microarray. The microarrays (ArrayTubes) hybridized 36 strains carrying that allowed testing...
A fourth type of RTX determinant was identified in Actinobacillus pleuropneumoniae and designated apxlVA. When expressed Escherichia coli, recombinant ApxlVA showed a weak haemolytic activity cohaemolytic synergy with the sphingomyelinase (beta-toxin) Staphylococcus aureus. These activities required presence an additional gene, ORF1, that is located immediately upstream The apxlVA gene product could not be detected A. cultures grown under various conditions vitro; however, pigs...
Sequences of the gene encoding β -subunit RNA polymerase ( rpoB ) were used to delineate phylogeny family Pasteurellaceae . A total 72 strains, including type strains major described species as well selected field isolates, included in study. Selection universal -derived primers for allowed straightforward amplification and sequencing a 560 bp fragment gene. In parallel, 16S rDNA was sequenced from all strains. The phylogenetic tree obtained with sequences reflected branches rDNA, especially...
Bacterial symbionts are crucial for the infectivity and success of entomopathogenic nematodes as biological control agents. The current understanding symbiotic relationships is limited by taxonomic uncertainties. Here, we used whole-genome sequencing traditional techniques to reconstruct phylogenetic between all described Photorhabdus species subspecies well 11 newly isolated bacteria Heterorhabditis nematodes, including unreported bacterial partner H. beicherriana. In silico DNA-DNA...
Campylobacteriosis is the most frequent zoonosis in developed countries and various domestic animals can function as reservoir for main pathogens Campylobacter jejuni coli. In present study we compared population structures of 730 C. coli from human cases, 610 chicken, 159 dog, 360 pig 23 cattle isolates collected between 2001 2012 Switzerland. All had been typed with multi locus sequence typing (MLST) flaB-typing their genotypic resistance to quinolones was determined. We used complementary...
Genetic relationships among bacterial strains belonging to the genus Aeromonas were inferred from 16S rRNA, gyrB and rpoB gene sequences. Twenty-eight type or collection of recognized species subspecies 33 isolated human animal specimens as well environmental samples included in study. As reported previously, rRNA sequence is highly conserved within Aeromonas, having only limited resolution for this very tight group species. Analysis a 1.1 kb confirmed that has high resolving power, with...
The sequences of the 16S rRNA genes (rrs genes) Clostridium chauvoei, causative agent blackleg in cattle, and phenotypically related organism septicum were determined. After amplification 1,507-bp PCR fragments from corresponding rrs genes, determined a single round sequencing by using conserved region primers. A sequence similarity analysis revealed close phylogenetic relationship C. chauvoei cluster I (M. D. Collins, P. A. Lawson, Willems, J. Cordoba, Fernandez-Garayzabal, Garcia, Cai, H....
Earlier investigations have shown that 'Haemophilus somnus', agni' and 'Histophilus ovis' represent the same species. In present investigation, taxonomic position of this species is explored further by sequencing 16S rRNA rpoB genes strains were investigated previously DNA–DNA hybridization. These results clearly support allocation to a novel genus within family Pasteurellaceae. The phenotypic separation Histophilus somni gen. nov., sp. nov. from other members can, for most strains, be based...
Principles and guidelines are presented to ensure a solid scientific standard of papers dealing with the taxonomy taxa Pasteurellaceae Pohl 1981 . The classification is in principle based on polyphasic approach. DNA sequencing certain genes very important for defining borders taxon. However, characteristics that common all members taxon which might be helpful separating it from related must also identified. Descriptions have as many strains possible (inclusion at least five highly...
Gram-negative, coccoid, non-motile bacteria that are catalase-, urease- and indole-negative, facultatively anaerobic oxidase-positive were isolated from the bovine rumen using an improved selective medium for members of Pasteurellaceae. All strains produced significant amounts succinic acid under conditions with glucose as substrate. Phenotypic characterization multilocus sequence analysis (MLSA) 16S rRNA, rpoB, infB recN genes performed on seven independent isolates. four showed high...
This investigation was based on 23 isolates from several European countries collected over the past 30 years, and included characterization of all isolates. Published data amplified fragment length polymorphism typing representing biovars as well protein profiles were used to select strains that then further characterized by polyamine profiling sequencing 16S rRNA, infB, rpoB recN genes. Comparison rRNA gene sequences revealed a monophyletic group within avian Pasteurellaceae, which...
Rodentibacter gen. nov. is proposed based on isolation and phenotypic characterization of strains, predominantly from rodents. The strains showed 86 % or higher rpoB gene sequence similarity indicated a genus-level relationship within Pasteurellaceae. compared at 16S rRNA level 93.8 similarity, their Pasteurellaceae was confirmed by analysis. type species pneumotropicus comb. reclassified [Pasteurella] pneumotropica with strain NCTC 8141T (=CCUG 12398T). Whole genomic comparison allowed the...
Carbohydrate-deficient glycoprotein syndrome (CDGS) represents a class of genetic diseases characterized by abnormal N-linked glycosylation. CDGS patients show large number abnormalities resulting in dysmorphy, encephalopathy, and other organ disorders. The majority CDGSs described to date are related an impaired biosynthesis dolichyl pyrophosphate-linked Glc 3 Man 9 GlcNAc 2 the endoplasmic reticulum. Recently, we identified four novel type accumulation . Elaborating on analogy this finding...
Phylogenies of housekeeping gene and 16S rRNA sequences were compared to improve the classification bacterial family Pasteurellaceae knowledge evolutionary relationships its members. Deduced partial protein genes atpD , infB rpoB in 28, 36 28 representative taxa respectively. The monophyly representatives genus Gallibacterium was recognized by analysis all genes, while members Mannheimia Actinobacillus sensu stricto core group Pasteurella formed monophyletic groups with two out three genes....