Abstract Background Control of brucellosis in livestock, wildlife and humans depends on the reliability methods used for detection identification bacteria. In present study, we describe evaluation recently established real-time PCR assay based Brucella -specific insertion sequence IS 711 with blood samples from 199 wild boars (first group animals) tissue 53 (second collected Switzerland. Results were compared to those obtained by bacterial isolation, Rose Bengal Test (RBT), competitive ELISA...

ABSTRACT The zoonotic pathogen Capnocytophaga canimorsus is part of the oral microbiome dogs and cats. We report genome sequence strain KM3195_24, isolated from a red fox. It has circular 2,718,701 bp with G + C content 36%, 2,419 CDS, 3 rRNA operons, 45 tRNAs.

We here describe a novel, Gram-stain positive, catalase positive and oxidase negative bacterium isolated from pyothorax samples of cats. The four described strains 14KM1171, 17KM0085, 22MD1345 22MD1512 T have genome size 2.35–2.40 Mb DNA G+C content 68.3–68.4 mol%. They are genetically most closely related to Buchananella hordeovulneris with 16S rRNA similarity 96.9%, digital DNA–DNA hybridization value 15% an average nucleotide identity 73.5%, all clearly below the species threshold. major...

New tetracycline and streptomycin resistance genes, tet(44) ant(6)-Ib, were identified in Campylobacter fetus subsp. within a transferable pathogenicity island that is typically unique to venerealis. The 640-amino-acid determinant, Tet 44, belongs class of proteins confers minocycline by ribosomal protection. 286-amino-acid ANT(6)-Ib, family aminoglycoside nucleotidyltransferases. phenotypes demonstrated gene inactivation expression.

The risk of transmission pathogens from free-ranging wild boars (Sus scrofa scrofa) to outdoor domestic pigs (S. domesticus) is increasing concern in many European countries. We assess this risk, using Switzerland as an example. estimated 1) the prevalence important and 2) interactions between pigs. First, we tested 252 selected areas 2008 2010 for infection with Brucella spp. Bacterial was 28.8% (confidence interval [CI] 23.0-34.0) when bacterial culture (B. suis Biovar real-time polymerase...

Gram-stain-positive cocci were isolated from miscellaneous sites of the skin healthy dogs as well infection in dogs. The closest relative by sequencing 16S rRNA gene was Macrococcus caseolyticus with 99.7 % sequence identity, but compared M. caseolyticus, novel strains shared only 90.8 to 93.5 DNA identity cpn60, dnaJ, rpoB and sodA partial genes, respectively. also exhibited differential phenotypic characteristics majority displayed a visible haemolysis on sheep blood agar, while did not...

Seven bacterial strains isolated from bovine endocarditis in six animals different geographic regions were investigated a polyphasic taxonomic approach. Phylogenetic analysis based on 16S rRNA gene sequences placed all seven isolates distinct, monophyletic cluster the family Neisseriaceae with closest similarity to type of Alysiella filiformis (97.06 %) and Kingella kingae (96.34 %). Whole genome sequence confirmed their species status, an average nucleotide identity >96 % between <80 other...

ABSTRACT Listeria monocytogenes is among the most important food-borne pathogens and well adapted to persist in environment. To gain insight into genetic relatedness potential virulence of L. strains causing central nervous system (CNS) infections, we used multilocus variable-number tandem-repeat analysis (MLVA) subtype 183 isolates, from ruminant rhombencephalitis some human patients, food, Allelic-profile-based comparisons grouped mainly three clonal complexes linked single-locus variants...

MRSA is an important human pathogen; thus, its increasing prevalence in livestock over the last decade has a potentially large impact on public health. Farmers and veterinarians are especially at risk due to their close contact with animals. Our work demonstrates dramatic increase Swiss pigs, from 2% 2009 44% 2017. Whole-genome sequencing allowed us show association between farmer pig strains as well veterinarian horse strains, indicating that respective animals likely source of...

'Treponema phagedenis' was originally described in 1912 by Noguchi but the name not validly published and no type strain designated. The taxon included Approved Lists of Bacterial Names hence has standing nomenclature. Six Treponema strains positive a 'T. phylogroup-specific PCR test were isolated from digital dermatitis (DD) lesions cattle further characterized compared with human ATCC 27087. Results phenotypic genotypic analyses including API ZYM, VITEK2, MALDI-TOF electron microscopy, as...

Pasteurellaceae are bacteria with an important role as primary or opportunistic, mainly respiratory, pathogens in domestic and wild animals. Some species of cause severe diseases high economic losses commercial animal husbandry great diagnostic concern. Because new data on the phylogeny Pasteurellaceae, their taxonomy has recently been revised profoundly, thus requiring improved phenotypic differentiation procedure to identify individual this family. A simplified Actinobacillus,...

We screened a total of 340 veterinarians (including general practitioners, small animal large working in different veterinary services or industry), and 29 assistants for nasal carriage methicillin-resistant Staphylococcus aureus (MRSA) pseudintermedius (MRSP) at the 2012 Swiss annual meeting. MRSA isolates (n = 14) were detected 3.8 % (95 CI 2.1 - 6.3 %) participants whereas MRSP was not detected. Large practitioners carriers livestock-associated (LA-MRSA) ST398-t011-V 2), ST398-t011-IV 4),...

Abortion in small ruminants presents a clinical and economic problem with legal implications regarding animal health zoonotic risk by some of the abortive pathogens. Several bacteria, fungi parasites can cause abortion, but cost-orientated routine diagnostics only cover most relevant epizootic agents. To broad-range common as well underdiagnosed abortifacients, we studied 41 ovine 36 caprine abortions Stamp's modification Ziehl-Neelsen stain, culture for classical opportunistic agents,...

Abstract Background Corynebacterium (C.) sp. 22KM0430 related to C. oculi and isolated from a dog exhibited resistance tetracycline, its WGS analysis revealed putative gene on 35 562-bp plasmid also harbouring the MLSB erm(X). Objectives To characterize novel tetracycline tet(65) demonstrate functionality by expression in glutamicum Escherichia coli curing of host strain. Methods was cloned with without repressor tetR(65) expressed DSM20300 E. DH5α. Plasmid cured non-selective passages....

The aim of this study was to determine the prevalence three Treponema species as well D. nodosus in Digital dermatitis (DD) and slurry Swiss cattle using PCR. A total 86 specimens from 24 farms were enrolled study. Slurry samples 21 DD-affected one unaffected farm collected assess potential environmental transmission. Nested real-time PCR performed detect nodosus, respectively. DD-stages positive for at least or more DD-associated 50 61 cases (82.0%) 9 25 (36.0%) animals. Infected animals...

A newly-discovered foot disease of unknown origin in captive European Bison ( bonasus ) was recently detected at Berne Animal Park. Dermatitis the interdigital cleft varying degrees severity diagnosed all animals n = 10). The aim this study to describe gross and histological lesions found 10 bison identify involved potential pathogens affected feet using molecular-based methods for Treponema spp., Dichelobacter nodosus Fusobacterium necrophorum . Lesions were scored according degree...

Several strains belonging to the genus Corynebacterium, but not any described species of were isolated from bovine mastitic milk samples over past five years in diagnostic unit University Bern. Six these (18M0132T, 17M2518, 18M0913, 19M0083, 20M1046 and 20M1090) that phenotypically similar further characterized genotypically. Gram-positive coryneform rods catalase positive, facultative anaerobe CAMP-test negative. Whole genome sequencing subsequent phylogenetic analysis revealed their size...

The cloning and molecular genetic analysis of a locus mapping within the flagellar gene ( fli ) complex Salmonella typhimurium is reported. A copy insertion element IS 200 was located in noncoding stretch DNA upstream fliA gene. Comparative nucleotide sequence showed that this 711 bp long its flanking regions contained no features common to other characterized sites element. 37 downstream an ORF whose product shown by interspecific transfer amino acid carry out N -methylation selected lysine...

Campylobacter fetus subspecies (CFF) is an important pathogen for both cattle and humans. We performed a systematic epidemiological clinical study of patients evaluated the genetic relatedness 17 human bovine CFF isolates by using different genotyping methods. In addition, serotype, dissemination genomic island containing type IV secretion system (T4SS) resistance determinants tetracycline streptomycin were also evaluated. The from diagnosed with infection as well those faecal samples...