A5048040571- Burkholderia infections and melioidosis
- Entomopathogenic Microorganisms in Pest Control
- Insect Resistance and Genetics
- Nematode management and characterization studies
- Microbial infections and disease research
- Amoebic Infections and Treatments
- Chemical Looping and Thermochemical Processes
- Immune Cell Function and Interaction
- Animal Disease Management and Epidemiology
- Brucella: diagnosis, epidemiology, treatment
- Insect symbiosis and bacterial influences
- Moringa oleifera research and applications
- Pancreatitis Pathology and Treatment
- Insect Pest Control Strategies
- Mollusks and Parasites Studies
- Insect and Arachnid Ecology and Behavior
- Extracellular vesicles in disease
- Viral Infections and Vectors
- Aquaculture disease management and microbiota
- Invertebrate Taxonomy and Ecology
- Insect and Pesticide Research
- Virology and Viral Diseases
- Bacteriophages and microbial interactions
- Aquatic Invertebrate Ecology and Behavior
- Plant and Fungal Interactions Research
Mahidol University
2017-2025
Mahidol Oxford Tropical Medicine Research Unit
2017-2025
University of Bern
2023-2024
Naresuan University
2017-2021
Naresuan University Hospital
2021
Melioidosis is a neglected tropical infection caused by the environmental saprophyte Burkholderia pseudomallei.We conducted prospective, observational study at nine hospitals in northeastern Thailand, hyperendemic melioidosis zone, to define current characteristics of patients and quantify outcomes over one year.2574 individuals hospitalised with culture-confirmed were screened 1352 analysed. The median age was 55 years, 975 (72%) male, 951 (70%) had diabetes. 565 (42%) presented lung...
Aedes aegypti is a potential vector of West Nile, Japanese encephalitis, chikungunya, dengue and Zika viruses. Alternative control measurements the are needed to overcome problems environmental contamination chemical resistance. Xenorhabdus Photorhabdus symbionts in intestine entomopathogenic nematodes (EPNs) Steinernema spp. Heterorhabditis These bacteria able produce broad range bioactive compounds including antimicrobial, antiparasitic, cytotoxic insecticidal compounds. The objectives...
Abstract The Mycoplasma Immunoglobulin Binding/Protease (MIB-MIP) system is a candidate ‘virulence factor present in multiple pathogenic species of the Mollicutes , including fast-growing feriruminatoris . MIB-MIP cleaves heavy chain host immunoglobulins, hence affecting antigen-antibody interactions and potentially facilitating immune evasion. In this work, using -omics technologies 5’RACE, we show that four copies M. have different expression levels are transcribed as operons controlled by...
Hemolysin co-regulated protein 1 (Hcp1) is a component of the cluster Type VI secretion system (T6SS1) that plays key role during intracellular lifecycle Burkholderia pseudomallei . Hcp1 recognized as promising target antigen for developing melioidosis diagnostics and vaccines. While gene encoding retained across B strains, variants hcp1 have recently been identified. This study aimed to examine prevalence in clinical isolates , assess antigenicity variants, ability strains expressing these...
<title>Abstract</title> Extracellular vesicles (EVs) are central components of bacterial secretomes, including the small, cell wall-less <italic>Mollicutes</italic>. Although EV release in <italic>Mollicutes </italic>has been reported, proteomic composition and function have not explored yet. We developed a protocol for isolating EVs pathogens <italic>Mycoplasma mycoides</italic>subsp. <italic>capri</italic> (<italic>Mmc</italic>)...
Photorhabdus and Xenorhabdus are symbiotic with entomopathogenic nematodes (EPNs) of the genera Heterorhabditis Steinernema, respectively. These bacteria produce several secondary metabolites including antimicrobial compounds. The objectives this study were to isolate identify EPNs their from Mae Wong National Park, Thailand evaluate antibacterial activities symbiont extracts against drug resistant bacteria. A total 550 soil samples 110 sites collected between August 2014 July 2015. EPN...
Objective: To evaluate the efficacy of symbiotic bacteria, Xenorhabdus indica, stockiae, Photorhabdus luminescens subsp. akhurstii and hainanensis as a larvicide against Aedes aegypti albopictus. Methods: Larvae (L3-L4) albopictus were given 2 mL suspension 107-108 CFU/mL each bacterium. Distilled water Escherichia coli ATCC® 25922 used control. The mortality rate larval mosquitoes was observed at 24, 48, 72 96 h. experiment performed in triplicates. Results: larvae both species started to...
Abstract Burkholderia pseudomallei is a flagellated, gram-negative environmental bacterium that causes melioidosis, severe infectious disease of humans and animals in tropical areas. We hypothesised B . may undergo phenotypic adaptation response to an increase growth temperature. analysed the curves strain 153 cultured Luria–Bertani broth at five different temperatures (25 °C–42 °C) compared proteomes bacteria 37 °C 42 °C. exhibited highest rate with modest reductions 30 °C, 40 but more...
Xenorhabdus and Photorhabdus, symbiotically associated with entomopathogenic nematodes (EPNs), produce a range of antimicrobial compounds. The objective this study is to identify Photorhabdus their EPNs hosts, which were isolated from soil samples Saraburi province, antibacterial activity against 15 strains drug-resistant bacteria. Fourteen isolates (6.1%), consisting six eight isolates, obtained 230 samples. Based on the BLASTN search incorporating phylogenetic analysis partial recA gene,...
Abstract Although cattle are the mammalian species with most global biomass associated a huge impact on our planet, their immune system remains poorly understood. Notably, bovine has peculiarities such as an overrepresentation of γδ T cells that requires particular attention, specifically in infectious context. In line 3R principles, we developed ex vivo platform to dissect host–pathogen interactions. The experimental design was based two independent complementary readouts: firstly, novel...
Xenorhabdus and Photorhabdus are gram negative bacteria that can produce several secondary metabolites, including antimicrobial compounds. They have a symbiotic association with entomopathogenic nematodes (EPNs). The aim of this study was to isolate identify species their associated nematode symbionts from Northeastern region Thailand. We also evaluated the antibacterial activity these bacteria. recovery rate EPNs 7.82% (113/1445). A total 62 51 strains were isolated EPNs. Based on recA...
Burkholderia pseudomallei is an environmental bacterium that causes melioidosis, a major community-acquired infection in tropical regions. Melioidosis presents with range of clinical symptoms, often characterized by robust inflammatory response, may relapse after treatment, and results high mortality rates. Lipopolysaccharide (LPS) B. potent immunostimulatory molecule comprised lipid A, core, O-polysaccharide (OPS) components. Four LPS types have been described based on SDS-PAGE patterns...
Melioidosis is an often lethal tropical disease caused by the Gram-negative bacillus, Burkholderia pseudomallei. The study objective was to characterize transcriptomes in melioidosis patients and identify genes associated with outcome. Whole blood RNA-seq performed a discovery set of 29 3 healthy controls. Transcriptomic profiles who did not survive 28 days were compared survived controls, showing 65 significantly up-regulated 218 down-regulated non-survivors survivors. Up-regulated involved...
Abstract Angiostrongylus cantonensis is primarily considered an emerging infectious agent of eosinophilic meningitis or meningoencephalitis with a worldwide distribution. Rodents and snails are important invasive hosts for transmission expansion A. . The objective this study was to investigate infection levels in snails, the most natural intermediate host. Our location Mueang Kamphaeng Phet district, Province, undertaken between October December 2012. A total 2228 freshwater terrestrial were...
Abstract Aedes aegypti is an insect vector that transmits several viruses affecting humans worldwide. Entomopathogenic nematodes (EPNs) and their symbiotic bacteria are organisms with the potential to control many insects. In this study, we did a survey aimed identify EPNs evaluate larvicidal activity of against Ae. . We collected 540 soil samples from 108 sites in Phitsanulok Province, lower northern Thailand. Baiting techniques White traps were used isolate samples. By sequencing 28S rDNA...
Introduction Melioidosis is an often-fatal tropical infectious disease caused by the Gram-negative bacillus Burkholderia pseudomallei , but few studies have identified promising biomarker candidates to predict outcome. Methods In 78 prospectively enrolled patients hospitalized with melioidosis, six candidate protein biomarkers, from literature, were measured in plasma at enrollment. A multi-biomarker model was developed using least absolute shrinkage and selection operator (LASSO)...
Melioidosis, a neglected tropical infection caused by Burkholderia pseudomallei, commonly presents as pneumonia or sepsis with mortality rates up to 50% despite appropriate treatment. A better understanding of the early host immune response melioidosis may lead new therapeutic interventions and prognostication strategies reduce disease burden. Whole blood transcriptomic signatures in 164 patients 70 other infections hospitalized northeastern Thailand enrolled within 24 hours following...
<title>Abstract</title> The Mycoplasma Immunoglobulin Binding/Protease (MIB-MIP) system is a candidate virulence factor present in multiple pathogenic species of the <italic>Mollicutes</italic>, including fast-growing <italic>Mycoplasma feriruminatoris</italic>. MIB-MIP cleaves heavy chain host immunoglobulins, hence affecting antigen-antibody interactions and potentially facilitating immune evasion. In this work we analyzed distribution genetic relatedness between systems different...
ABSTRACT. Melioidosis, infection caused by Burkholderia pseudomallei , is characterized robust innate immune responses. We have previously reported associations of TLR1 single nucleotide missense variant rs76600635 with mortality and TLR5 nonsense rs5744168 both bacteremia in single-center studies patients melioidosis northeastern Thailand. The objective this study was to externally validate the a large multicenter cohort patients. genotyped 1,338 enrolled prospective parent conducted at...
Sepsis is a complex heterogeneous condition, and the current lack of effective risk outcome predictors hinders improvement its management. Using reductionist approach leveraging publicly available transcriptomic data, we describe knowledge gap for role ACVR1B (activin A receptor type 1B) in sepsis. ACVR1B, member transforming growth factor-beta (TGF-beta) superfamily, was selected based on following: 1) induction upon vitro exposure neutrophils from healthy subjects with serum septic...
ABSTRACT Although cattle are the mammalian species with most global biomass associated a huge impact on our planet, their immune system remains poorly understood. Notably, bovine has peculiarities such as an overrepresentation of γδ T cells that requires particular attention, specifically in infectious context. In line 3R principles, we developed ex vivo platform to dissect host-pathogen interactions. The experimental design was based two independent complementary readouts: firstly, novel...